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Cadka Animal Direct kit PCR kit-UNG
Qalabkani wuxuu isticmaalaa nidaam kayd ah oo loo yaqaan 'lysis buffer system' si dhakhso leh looga sii daayo hidde-sideha DNA-da shaybaarka unugyada xoolaha ee falcelinta PCR, markaa waxay si gaar ah ugu habboon tahay baaritaanka hidda-socodka ee ballaaran.
Nidaamka lagu sii daayo hidde-sideha hidde-wadaha ee dheecaanka lysis ayaa lagu dhammeeyaa 10-30 daqiiqo gudahood 65°C. Majiro waxqabadyo kale sida borotiinka iyo saarista RNA, iyo raadadka la sii daayay ee DNA ayaa loo isticmaali karaa tusaale ahaan falcelinta PCR.
2×PCR fududTM Isku-darka (UNG) wuxuu adeegsadaa dUTP halkii uu ka isticmaali lahaa dTTP asalka 2×PCR fududTM Isku qas, oo ku dar enzyme UNG (Uracil-N-glycosylase) oo hoos u dhigi kara shaybaarka ay ku jirto dUTP isla waqtigaas. Kahor falcelinta PCR, enzyme-ka UNG waxaa loo isticmaalaa in lagu duleeyo badeecada PCR ee uracil ku jiro. UNG enzyme wax saameyn ah kuma yeelan doono shaxanka aan ku jirin uracil, taas oo hubineysa qeexnaanta iyo saxsanaanta kordhinta iyo ka hortagga suurtagalnimada in alaabada PCR ay sumowdo inta lagu jiro baaritaanka hidaha ee ballaaran.
D-Taq DNA polymerase waa DNA polymerase si gaar ah loogu sameeyay Foregene falcelinta tooska ah ee PCR. D-Taq DNA polymerase wuxuu leeyahay dulqaad xoog leh noocyada kala duwan ee ka hortagayaasha falcelinta PCR, wuxuuna si wax ku ool ah u kordhin karaa tirada raadraaca DNA ee nidaamyada kala duwan ee falcelinta isku dhafan, iyo xawaaraha ballaadhintu wuxuu gaari karaa 2Kb / min, kaas oo si gaar ah ugu habboon falcelinta tooska ah ee PCR.
