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Cadka Xayawaanka Xirmada Qalabka PCR
Qalabkani wuxuu isticmaalaa nidaam kayd ah oo loo yaqaan 'lysis buffer system' si dhakhso leh looga sii daayo hidde-sideha DNA-da shaybaarka unugyada xoolaha ee falcelinta PCR, markaa waxay si gaar ah ugu habboon tahay baaritaanka hidda-socodka ee ballaaran.
Nidaamka lagu sii daayo hidde-sideha hidde-wadaha ee dheecaanka lysis ayaa lagu dhammeeyaa 10-30 daqiiqo gudahood 65°C. Majiro waxqabadyo kale sida borotiinka iyo saarista RNA, iyo raadadka la sii daayay ee DNA ayaa loo isticmaali karaa tusaale ahaan falcelinta PCR.
2× PCR fududTM Isku-darka wuxuu leeyahay dulqaad xoog leh oo ku saabsan ka-hortagayaasha fal-celinta PCR, waxayna u isticmaali kartaa lakabka muunadda in lagu tijaabiyo qaab-dhismeedka wax-ku-oolnimada iyo ballaarinta gaarka ah. Reagent-kan wuxuu ka kooban yahay Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, keydka falcelinta, hagaajiyaha PCR iyo xasiliyaha.
D-Taq DNA polymerase waa DNA polymerase si gaar ah loogu sameeyay Foregene falcelinta tooska ah ee PCR. D-Taq DNA polymerase wuxuu leeyahay dulqaad xoog leh noocyada kala duwan ee ka hortagayaasha falcelinta PCR, wuxuuna si wax ku ool ah u kordhin karaa tirada raadraaca DNA ee nidaamyada kala duwan ee falcelinta isku dhafan, iyo xawaaraha ballaadhintu wuxuu gaari karaa 2Kb / min, kaas oo si gaar ah ugu habboon falcelinta tooska ah ee PCR.
