Xirmada Go'doominta DNA ee Unugyada Xayawaanka ee Shiinaha Xirmooyinka Soo-saarista Unugyada Hilibka ee Muunadaha Unugyada Xoolaha Soo Saaraha iyo Iibiyaha |Foregene

Xirmooyinka Go'doominta DNA Nudaha Xayawaanka Xirmooyinka Soo-saarista DNA ee Muunadaha Unugyada Xoolaha

Xirmada Go'doominta DNA ee Nudaha Xayawaanka Xirmooyinka Soo saarista DNA ee Tusaalooyinka Nudaha Xayawaanka

Sharaxaada Qalabka:

Soo saarista degdega ah iyo nadiifinta DNA-da genomic ee ilo badan, sida unugyada xayawaanka, unugyada, iwm.

◮Xawaaraha dheereeya:Dhaqdhaqaaq ka sarreeya borotiinka la midka ah oo si dhakhso ah u dheefshiido shaybaarada unugyada;

◮Fudud:waxa lagu dhamayn karaa 50 daqiiqo.

◮Ku habboon:Lagu sameeyay heerkulka qolka.

◮Badbaadada:looma isticmaalo reagent organic.

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Faahfaahinta Alaabta

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Sharaxaada Kit

Xirmadani waxay isticmaashaa tiir DNA-kaliya oo si gaar ah u xidhi kara DNA-da, Protease Foregene iyo nidaam kayd gaar ah.DNA-ga genomic-ga tayada sare leh waxaa laga soo saari karaa unugyo dhaqan oo kala duwan iyo unugyo xayawaan ah 30 ilaa 50 daqiiqo gudahood.

Xuubabka silica-ga DNA-ga ah ee loo isticmaalo tiirka lafdhabarta ayaa ah walaxda cusub ee Foregene, kaas oo si wax ku ool ah oo gaar ah ugu xidhi kara DNA-da, oo kor u qaadi karta ka saarida RNA, borotiinnada wasakhaysan, ions iyo xeryahooda kale ee organic ee unugyada.5-80μg DNA genomic oo tayo sare leh ayaa laga nadiifin karaa unug dhan 10-50mg.

Tilmaamaha

Qalabka Go'doominta Unugyada Xoolaha
Waxa ku jira Kit	DE-05011	DE-05012	DE-05013
Waxa ku jira Kit	50T	100T	250T
Buffer L1	20ml	40ml	100ml
Buffer L2*	20ml	40ml	100ml
Buffer PW*	25ml	50ml	125ml
Buffer WB	25ml	50ml	125ml
Buffer EB	10ml	20ml	50ml
Kahortagga Protease	1.25ml	2.5ml	6.5ml
Tiirka-Kaliya DNA	50	100	250
Buugga	1	1	1

*: Buffer L2 iyo Buffer PW waxa ku jira cusbo macmala ah oo xanaaqsan.Fadlan xidho galoofyada oo qaado talaabooyinka ilaalineed ee khuseeya markaad shaqaynayso.

Tilmaamaha & faa'iidooyinka

■ Wax wasakhaysan RNase ma jiro: Tiirka DNA-kaliya ee lagu bixiyo xirmada ayaa suurtogal ka dhigaysa in RNA laga saaro DNA-da genomic iyada oo aan la helin RNase dheeraad ah inta lagu jiro tijaabada, si looga hortago shaybaarka in uu wasakheeyo RNase dibadda ah.

■ Xawaaraha dheereeya: Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah wuxuuna si dhakhso ah u dheefshiidaa shaybaarka nudaha.

■ Fudud: hawlgalka soo saarista DNA-ga genomic waxa lagu dhamayn karaa 50 daqiiqo gudahood.

■ Ku habboon: Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, looma baahna 4℃ sentrifugation heerkulka hooseeya ama roobab ethanol ah oo loo baahan yahay.

■ Badbaadada: looma baahna reageent organic.

■ Tayada sare: DNA-da genomic-ga la soo saaray ayaa leh jajabyo waaweyn, malaha RNA, malaha RNase, iyo maadada ion aad u hooseysa, waxay buuxin kartaa shuruudaha tijaabooyinka kala duwan.