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Qalabka Tooska ah ee loo yaqaan 'RT qPCR Kit'
Qalabkani wuxuu adeegsanayaa nidaam keyd u ah leyse u gaar ah oo si dhakhso leh uga sii deyn kara RNA shaybaarka unugyada dhaqanka leh ee fal-celinta RT-qPCR, taas oo meesha ka saaraysa hawsha nadiifinta RNA ee waqtiga qaadata iyo hawlkarnimada badan. Template-ka RNA waxaa lagu heli karaa kaliya 7 daqiiqo. 5 × Direct RT Mix iyo 2 × Tooska qPCR Mix-SYBR reagent ee ay bixiso xirmada ayaa si dhakhso leh oo wax ku ool ah u heli kara natiijooyinka waqtiga dhabta ah ee PCR.
5 × Direct RT Mix iyo 2 × Direct qPCR Mix-SYBR waxay leeyihiin dulqaad xakameyn xoog leh, lysate-yada shaybaarka waxaa si toos ah loogu adeegsan karaa tusaale ahaan RT-qPCR. Qalabkani wuxuu ka kooban yahay isku-xidhka sare ee 'RNA' ee 'Foregene' transcriptase transcriptase, iyo Hot D-Taq DNA polymerase, dNTPs, MgCl2, falcelinta keydiyaha, hagaajinta PCR iyo xasiliyaha.
