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Lnc-RT Heroᵀᴹ I (oo leh gDNase) (Super Premix oo loogu talagalay isku-darka cDNA ee koowaad-silsiladda laga bilaabo lncRNA)
Halyeey Lnc-RTTM I (Iyadoo leh gDNase) waa nidaam soo-celin celin ah oo si gaar ah loogu sameeyay lncRNA si dhakhso leh looga saaro wasakheynta hiddo-sidaha DNA. 5 × gDNase Mix wuxuu si dhakhso leh uga saari karaa genome-ka ku haray RNA 42 ° C 2 daqiiqo, si wax ku ool ah ayuu uga fogaan karaa faragalinta genome ee natiijooyinka qPCR.
5 Hero Halyeeyga L-RTTM Isku-darka waxaa ku jira Foregene LncRNA Transverse Transcriptase oo si gaar ah loogu sameeyay Foregene, taas oo ah nooc cusub oo transcriptase gaar ah oo loogu talagalay lncRNA iyo qaab-dhismeedyo kale oo dhaadheer oo RNA ah, oo leh xiriir RNA oo xoog leh iyo wax-ku-oolnimada duubista sare. Nidaamka la habeeyay ayaa ka dhigaya sicirka transcript-ka mid dhakhso badan wuxuuna si fudud u qori karaa qaab-dhismeedka RNA oo leh maaddo sare oo GC ah iyo qaab dhismeedka labaad ee adag. Qalabka ugu horreeya ee cDNA waxaa lagu dhammaystiri karaa 42 ° C 15 daqiiqo.
