China Mouse Tail Direct PCR Kit Direct PCR Lysis Reagent (Mouse Tail) (oo loogu talagalay Genotyping) Soo saaraha iyo Iibiyaha |Foregene

Mouse Tail Direct PCR Kit Tooska PCR Lysis Reagent(Mouse Dabada)

Mouse Tail Direct PCR Kit Tooska PCR Lysis Reagent(Mouse Tail)(oo loogu talagalay Genotyping) Sawirka sifaysan

Sharaxaada Qalabka:

Si toos ah u isticmaal jiirka dabada lysate qaab tusaale ah oo loogu talagalay kordhinta PCR, iyada oo aan la soo saarin DNA gaar ah, degdeg iyo dareen sare.

◮Looma baahna nadiifin waqti-qaadasho iyo qaali ah.

◮Dalabka muunadku wuu yar yahay, kaliya qaado hal abuur.

◮Looma baahna daaweyn gaar ah sida shiidi iyo burburin, qalliinkuna waa sahlan yahay.

◮Nidaamka PCR ee la hagaajiyay wuxuu awood u siinayaa PCR inuu yeesho gaar gaar ah iyo dulqaad adag oo loogu talagalay horjoogayaasha falcelinta PCR.

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Faahfaahinta Alaabta

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Sharaxaada

Xirmadani waxay isticmaashaa nidaam u gaar ah lysis-ka si ay si dhakhso ah uga sii daayaan DNA-da genomic ee dabada, dhegaha, murqaha iyo muunadaha kale ee unugyada ee falcelinta PCR, sidaas darteed waxay si gaar ah ugu habboon tahay baaritaanka hidde-sidaha ballaaran.

Habka looga sii daayo DNA-da genomic ee kaydiyaha lysis waxa lagu dhammeeyaa 10-30 daqiiqo gudahood 65°C.Looma baahna habab kale sida borotiinka iyo ka saarista RNA, DNA-da raadraaca ee la sii daayayna waxaa loo isticmaali karaa qaab-dhismeedka falcelinta PCR.

2× M1-PCR Fudud^TMIsku-darku waxa uu dulqaad xooggan u leeyahay hor-joogayaasha falcelinta PCR, waxayna isticmaali kartaa lysate-ka muunadda si loogu tijaabiyo qaab-dhismeedka si hufan oo gaar ah.Dib-u-kicinta waxay ka kooban tahay Foregene D-Taq DNAPolymerase, dNTPs, MgCl₂, celiyaha falcelinta, PCR optimizer iyo stabilizer.

D-Taq DNA polymerase waa DNA polymerase si gaar ah u soo saaray Foregene ee falcelinta PCR tooska ah.D-Taq DNA polymerase waxay leedahay dulqaad xoog leh oo loogu talagalay xakameynta falcelinta PCR ee kala duwan, waxayna si wax ku ool ah u kordhin kartaa tirada DNA-da ee hababka falcelinta kakan ee kala duwan, iyo xawaaruhu wuxuu gaari karaa 2Kb/min, kaas oo si gaar ah ugu habboon falcelinta PCR tooska ah.

Tilmaamaha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Qaybaha kitka

Qaybta I	Xildhiban Buffer
	Foregene Protease Plus
	6× DNA Loading Buffer
Qaybta II	2× M1 PCR Fudud^TMIsku qas
Tilmaamaha

Tilmaamaha & faa'iidooyinka

■Looma baahna nadiifin waqti-qaadasho iyo qaali ah.

■ Baahida muunada waa yar tahay, in yar oo ah 5mg mouse tissue ama 2-5mm dabada jiirka ayaa la tijaabin karaa.

■ Looma baahna daawayn gaar ah sida shiididda iyo burburinta, qalliinkuna waa sahlan yahay.

■ Nidaamka PCR ee la hagaajiyay wuxuu ka dhigayaa PCR inay yeeshaan gaar gaar ah oo adag iyo dulqaad adag oo loogu talagalay horjoogayaasha falcelinta PCR.

Xuduudaha Kit

Codsiga: dabada jiirka, dhegaha jiirka, muruqa iyo unugyo kale.

DNA-da laga sii daayay muunada lysis: kaliya waxaa loo adeegsaday qaab PCR.

Qalabka waxaa loo isticmaali karaa ujeedooyinka soo socda: aqoonsiga transgenosis, genotyping xayawaanka, iwm.

Socodka shaqada

Kaydinta iyo nolosha shelf

Qaybta I ee xirmadan waa in lagu kaydiyaa 2-8℃.

Reagent Buffer AL waxa lagu kaydin karaa 12 bilood xaaladaha qalalan;waxa lagu kaydin karaa 2-8℃kaydinta dheer.

Reagent Foregene Protease wuxuu leeyahay qaacido gaar ah.Si loo hubiyo dhaqdhaqaaqeeda iyo xasiloonideeda, fadlan ku kaydi 4℃muddo 12 bilood ah.

Reagent 6×Kaydinta DNA-da waxa lagu kaydin karaa 4℃ama -20℃muddo dheer.

Qaybta II ee xirmadan waa in lagu kaydiyaa -20℃.

Reagent 2×M1 PCR Fudud^TMIsku qas, haddii si joogto ah loo isticmaalo, waxa kale oo lagu kaydin karaa 4℃kaydinta muddada gaaban (ku isticmaal ilaa 10 maalmood gudahood).

Hore: Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Mouse Tail)(loogu talagalay Genotyping)

Xiga: Xirmada PCR-da Tooska ah ee Unugyada Xayawaanka (la'aanteed Qalabka Muunalada) Hab-maamuuska

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