China Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Mouse Tail)(loogu talagalay Genotyping) Soo saaraha iyo Iibiyaha |Foregene

Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Mouse Tail)(loogu talagalay Genotyping)

Mouse Tail Direct PCR Kit-UNG Direct PCR Lysis Reagent(Mouse Tail)(oo loogu talagalay Genotyping) Sawirka

Sharaxaada Qalabka:

Dabada jiirka ee lysate waxaa si toos ah loo isticmaalaa sidii qaab tusaale ah oo loogu talagalay kordhinta PCR iyada oo aan la soo saarin DNA gaar ah, taas oo ah mid degdeg ah oo aad xasaasi u ah.Waxaa lagu daray UNG nidaamka ka-hortagga wasakheynta si meesha looga saaro waxyaabaha beenta ah.

◮Looma baahna nadiifin waqti-qaadasho iyo qaali ah.

◮Dalabka muunadku wuu yar yahay, kaliya qaado hal abuur.

◮Looma baahna daaweyn gaar ah sida shiidi iyo burburin, qalliinkuna waa sahlan yahay.

◮Nidaamka PCR ee la hagaajiyay wuxuu awood u siinayaa PCR inuu yeesho gaar gaar ah iyo dulqaad adag oo loogu talagalay horjoogayaasha falcelinta PCR.

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Sharaxaada

Xirmadani waxay isticmaashaa nidaam u gaar ah lysis-ka si ay si dhakhso ah uga sii daayaan DNA-da genomic ee dabada, dhegaha, murqaha iyo muunadaha kale ee unugyada ee falcelinta PCR, sidaas darteed waxay si gaar ah ugu habboon tahay baaritaanka hidde-sidaha ballaaran.

Habka looga sii daayo DNA-da genomic ee kaydiyaha lysis waxa lagu dhammeeyaa 10-30 daqiiqo gudahood 65°C.Looma baahna habab kale sida borotiinka iyo ka saarista RNA, DNA-da raadraaca ee la sii daayayna waxaa loo isticmaali karaa qaab-dhismeedka falcelinta PCR.

2× M1-PCR Fudud^TMMix (UNG) waxay isticmaashaa dUTP halkii ay ka isticmaali lahayd dTTP iyadoo ku saleysan 2× M1-PCR Easy^TMIsku qas (UNG), oo ku dara UNG enzyme (Uracil-N-glycosylase) kaas oo hoos u dhigi kara template ka kooban dUTP isku mar.Kahor falcelinta PCR-ga, ensaymka UNG waxaa loo isticmaalaa hoos u dhigista alaabta PCR ee ay ku jirto uracil.Enzyme UNG wax saameyn ah kuma yeelan doono qaab-dhismeedka aan ku jirin uracil, si loo hubiyo gaarnimada iyo saxnaanta xoojinta iyo ka hortagga suurtagalnimada baaritaanka hidda-socodka ballaaran.Wasakhaynta alaabta PCR ayaa dhacday.

D-Taq DNA polymerase waa DNA polymerase si gaar ah u soo saaray Foregene ee falcelinta PCR tooska ah.D-Taq DNA polymerase waxay leedahay dulqaad xoog leh oo loogu talagalay xakameynta falcelinta PCR ee kala duwan, waxayna si wax ku ool ah u kordhin kartaa tirada DNA-da ee hababka falcelinta kakan ee kala duwan, iyo xawaaruhu wuxuu gaari karaa 2Kb/min, kaas oo si gaar ah ugu habboon falcelinta PCR tooska ah.

Tilmaamaha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Qaybaha kitka

Qaybta I	Xildhiban Buffer
	Foregene Protease Plus
	6× DNA Loading Buffer
Qaybta II	2× M1 PCR Fudud^TMIsku qas(UNG)
Tilmaamaha

Tilmaamaha & faa'iidooyinka

■Looma baahna nadiifin waqti-qaadasho iyo qaali ah.

■ Baahida muunada waa yar tahay, in yar oo ah 5mg mouse tissue ama 2-5mm dabada jiirka ayaa la tijaabin karaa.

■ Looma baahna daawayn gaar ah sida shiidi iyo burburin, qaliinkuna waa sahlan yahay.

■ Nidaamka PCR ee la hagaajiyay wuxuu ka dhigayaa PCR inay yeeshaan gaar gaar ah oo adag iyo dulqaad adag oo loogu talagalay horjoogayaasha falcelinta PCR.

■ Nidaamka PCR-ka-hortagga wasakheynta 2×M1-PCR Fudud^TMIsku qas (UNG), kaas oo si wax ku ool ah u baabi'iya wasakhowga ay keenaan alaabada PCR oo hubisa gaar ahaan iyo saxnaanta kordhinta.

Xuduudaha Kit

Baaxadda codsiga: dabada jiirka, dhegta jiirka, muruqa iyo unugyo kale.

DNA-da laga sii daayay muunada lysis: kaliya waxaa loo adeegsaday qaab PCR.

Qalabka waxaa loo isticmaali karaa ujeedooyinka soo socda: aqoonsiga transgenosis, genotyping xayawaanka, iwm.

Socodka shaqada

Kaydinta iyo nolosha shelf

Qaybta I ee xirmadan waa in lagu kaydiyaa 2-8℃.

Reagent Buffer AL waxa lagu kaydin karaa 12 bilood xaaladaha qalalan;waxa lagu kaydin karaa 2-8℃ kaydinta dheer

Reagent Foregene Protease wuxuu leeyahay qaacido gaar ah.Si loo hubiyo dhaqdhaqaaqeeda iyo xasiloonideeda, fadlan ku kaydi at 4℃ ilaa 12 bilood.

Reagent 6×DNA Loading Buffer waxa lagu kaydin karaa 4℃ ama -20℃ wakhti dheer.

Qaybta II ee xirmadan waa in lagu kaydiyaa -20℃.

Reagent 2×M1 PCR EasyTM Mix(UNG), haddii si joogta ah loo isticmaalo, waxa kale oo lagu kaydin karaa 4℃ kaydinta muddada gaaban (isticmaal 10 maalmood gudahood).

Hore: Mouse Tail SuperDirect PCR Kit Direct PCR Lysis Reagent(Mouse Tail)(loogu talagalay Genotyping)

Xiga: Mouse Tail Direct PCR Kit Tooska PCR Lysis Reagent(Mouse Dabada)

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