Halyeyga PCR (oo leh Dye)
Tilmaamaha
5ml, 10ml, 40ml
Xirmada soo saarista Halyeyga PCR (With Dye) waxa ku jira jiilka cusub ee u gaarka ah ee Foregene Taq-Foregene Taq Hero, dNTP-yada la hagaajiyay, MgCl2, falcelinta falcelinta, kor u qaadka falcelinta PCR, hagaajinta iyo xasiliyaha.Nidaamka 2× PCR HeroTM Mix (dheeyuhu) wuxuu leeyahay dulqaad sare oo loogu talagalay horjoogayaasha PCR marka loo eego nidaamka isku-dhafka PCR ee caadiga ah, wuxuuna si fudud ula qabsan karaa PCR-weyneynta qaabab kala duwan oo adag.Nidaamka falcelinta gaarka ah iyo waxtarka sare ee Taq Hero ayaa ka dhigaya falcelinta PCR inay yeelato hufnaan sare u qaadis, gaar ahaan iyo dareen.
Qaybaha kitka
0.1U Taq Hero/μl |
400μM dNTPs |
20mM Tris-HCl (pH8.8) |
100mM KCl |
5mM mgCl2 |
Xasijiyeyaasha kale iyo kor u qaadayaasha |
Dejinta DNA-da |
Tilmaamaha & faa'iidooyinka
■Kalsoonida sare: 6 jeer insaymamka Taq caadiga ah;
■ Xawaaraha cod-weyneyntu waa 5-10sec/kb, kaas oo 6-12 jeer ka badan kuwa caadiga ah ee Taq;
■ Laqabsiga template oo badan: Waxay si hufan u kordhin kartaa qaabab kala duwan oo isku dhafan DNA-ga oo leh heer sarenuxurka GC;
■ Hufnaanta sare-u-qaadista: wareegyada cod-weyneysiinta oo ka hooseeya kuwa caadiga ah ee Taq;
■ Dulqaadka deegaanku wuu ka xoog badan yahay: waxaa lagu meeleeyaa 37°C muddo toddobaad ah, ilaalinta in ka badan 90% dhaqdhaqaaqa;
■ Waxay leedahay 5'→3'Dhaqdhaqaaqa DNA polymerase iyo 5'→3'dhaqdhaqaaqa exonuclease, oo aan lahayn 3'→5'dhaqdhaqaaqa exonuclease.
■ Qalabka waxaa horay loogu daray Electrophoresis Loading Buffer, ka dib marka PCR la dhammeeyo, muunada waxaa si toos ah loogu shubi karaa koronto-fayras;Qalliinku waa mid fudud oo fudud.
Codsiga qalabka
Kordhinta PCR ee jajabyada DNA-da, calaamadinta DNA-da, taxanaha DNA, PCR iyo dabada A
Kaydinta iyo nolosha shelf
Waxaa lagu kaydin karaa waqti dheer at -20°C;Isticmaalka joogtada ah, waxa lagu kaydin karaa 4°C muddo gaaban.