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Qalabka Nadiifinta PCR Nidaamka Nadiifinta PCR ee DNA-da saafiga ah
Si dhakhso leh u nadiifi oo u hel jajabyada DNA-da tayada sare leh ee nidaamka PCR;Haddii aad rabto inaad kala tagto oo aad hesho jajab DNA oo gaar ah, fadlan dooro xirmada soo kabashada jel.
◮ Soo kabashada DNA-da oo ballaadhan: Qaybaha DNA-da oo gaaban sida 60bp iyo inta 10kb ah waa la soo ceshan karaa.
◮ Waxtarka soo kabashada sareWaxtarka soo kabashada guud ahaan waa in ka badan 80%, kan ugu sarreeyana wuxuu gaari karaa in ka badan 95%.
Xawaaraha dheereeya:Fudud in la shaqeeyo, soo kabashada jajabka DNA-da waxaa lagu dhameyn karaa 15 daqiiqo gudahood iyo dimer-ka si toos ah ayaa loo saari karaa iyada oo aan dib u soo kabashada jel.
◮ SafeefSoo saarista reagent organic looma baahna.
◮ Tayo sare: Qaybaha DNA-da ee la helay waa nadiif sare, kuwaas oo la kulmi kara tijaabooyin kala duwan oo xiga.
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