Caleenta caleenta tooska ah ee PCR kit-UNG (la'aanteed Aalado Muunad) Hab-maamuuska tooska ah PCR ee agabka dhirta
Sharaxaada Qalabka:
Si toos ah u isticmaal caleen ama lysate caleen qaab template ah kor u qaadida PCR, iyada oo aan la soo saarin DNA gaar ah, degdeg iyo dareen sare.Nidaamka ka hortagga wasakheynta ee UNG ayaa lagu daray si looga hortago waxyaabo been abuur ah.
◮Ma jiro nadiifinta DNA-da waqti-qaadashada iyo qaaliga ah (Waqtiga, shaqada iyo kharashka la badbaadiyay!)
◮Wax yar
◮Fudud - muunada waxaa lagu soo rogi karaa falcelinta PCR ama falcelinta lysis iyada oo aan la jarin ama la shiidi
◮2 X Isku darka — yaraynta khaladaadka rarka muunada iyo wakhtiga diyaarinta nidaamka falcelinta
◮Diyaarinta qaab-dhismeed degdeg ah ayaa lagu dhammeyn karaa 10 daqiiqo, falcelinta PCR waxaa lagu dhameyn karaa 50 daqiiqo
◮Wax soo saarka sare — falcelinta lysis waxaa lagu dhammayn karaa saxan PCR 96-wanaagsan
Sharaxaada
Iyada oo ku saleysan xirmada caleenta tooska ah ee PCR, dUTP waxaa loo isticmaalaa halkii dTTP, iyo UNG ensaymes oo hoos u dhigi kara template ka kooban dUTP ayaa lagu daray.Sidan, gaar ahaan iyo saxnaanta xoojinta ayaa la hubiyaa, iyo dhibaatada wasakheynta alaabta PCR ee dhici karta inta lagu jiro baaritaanka hidda-socodka ballaaran waa laga hortagayaa.
Tilmaamaha
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Qaybaha kitka
| Qaybta I | Buffer P1 |
| Buffer P2 | |
| 6×Dejinta DNA-da | |
| Qaybta II | 2× Caleen PCR FududTMIsku qas(UNG) |
Tilmaamaha & faa'iidooyinka
■ Ma jiro waqti-qaadasho iyo nadiifin DNA-da qaali ah
■ Waxyaabo yar
■ Fudud-muunada waxaa lagu soo rogi karaa falcelinta PCR ama falcelinta lysis iyada oo aan la jarin ama la shiidi
■ 2 × Isku qas, yaraynta khaladaadka muunada rarida iyo wakhtiga diyaarinta nidaamka falcelinta
■ Diyaarinta qaab-dhaqso leh ayaa lagu dhammeyn karaa 10 daqiiqo, falcelinta PCR waxay ku dhameyn kartaa ugu yaraan 50 daqiiqo
■ Dareen-celinta-lysis-ka sare ee fal-celinta waxaa lagu dhammayn karaa saxan PCR 96-wanaagsan
Xuduudaha Kit
Codsiga: Aqoonsiga hidde ahaan la beddelay, genotyping, iwm.
Tusaale: caleen geed
Qiyaasta: Dhexroorka 2-3mm (hab toos ah), dhexroor 5-7mm (qaabka lysis)
Qiyaasta ogaanshaha: jajabka bartilmaameedka ≤1kb
Socodka shaqada
Electroferogram
Laba toddobaad oo ah natiijooyinka ogaanshaha isku dhafka PCR ee hoos yimaada xaaladda wasakhaynta alaabta PCR.
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