Qalabka PCR ee wakhtiga dhabta ah Easyᵀᴹ-SYBR Cagaaran I xirmada
Sharaxaada Qalabka:
Fudud-2X PCR Isku-dhafan si loo yareeyo khaladka tijaabada iyo wakhtiga hawlgalka
Bakhaar gaar ah oo la hagaajiyay iyo bilowga kulul ee Taq enzyme waxay ka hortagi kartaa kordhinta aan gaarka ahayn iyo samaynta dimer ka
Dareenka sare-wuxuu ogaan karaa nuqullo hoose oo template ah
Kala duwanaansho wanaagsan - oo ku habboon inta badan qalabka PCR ee tirada dhabta ah
Sharaxaada Kit
2× Real PCR FududTMMix-SYBR oo ay bixiso PCR-da-waqtiga dhabta ahTM-SYBR Cagaaran I xirmada waa nidaam horudhac ah oo cusub oo isticmaala SYBR Cagaaran I ee waqtiga-dhabta ah falcelinta PCR-ga, kaas oo si weyn u wanaajin kara gaarnimada badeecada iyo dareenka falcelinta.ROX waxa loo bixiyaa sidii dheeha xakamaynta gudaha.Xoogga fluorescence ee xirmadani waa 3-5 jeer ka alaabta la midka ah, taas oo noqon karta mid aad xasaasi u ah oo si dareen leh ka tarjumaysa diirada DNA template bartilmaameedka.
2× Real PCR FududTMMix-SYBR waxa ku jira bilowga kulul ee Foregene Taq DNA Polymerase.Marka la barbar dhigo enzymes-ka caadiga ah ee Taq, waxay leedahay faa'iidooyinka hufnaanta sare u qaadida, awood xoog leh oo gaar ah oo xoog leh iyo heerar is-gaarsiineed oo hooseeya.Waxay yarayn kartaa kordhinta aan gaarka ahayn waxayna hagaajin kartaa saxnaanta PCR.
Tilmaamaha
| Waqtiga dhabta ah PCR Easyᵀᴹ-SYBR Cagaarka I Kit | ||||
| Halabuurka Kit (Nidaamka 20 μl) | QP-01011 | QP-01012 | QP-01013 | QP-01014 |
| 200T | 500T | 1000T | 2000T | |
| 2× Real PCR EasyTMIsku-dhafka-SYBR | 1 ml × 2 | 1.7 ml × 3 | 1.7 ml × 6 | 1.7 ml × 12 |
| 50× ROX Tixraaca Midabka | 400 μl | 1 ml | 1 ml × 2 | 1 ml × 4 |
| DNA-free ddH2O | 1.7 ml | 1.7 ml × 2 | 10 ml | 20 ml |
| Tilmaanta | 1 | 1 | 1 | 1 |
Tilmaamaha & faa'iidooyinka
■ Fudud-2X PCR Isku-dhafan si loo yareeyo khaladka tijaabada iyo wakhtiga hawlgalka
■ Bakhaar gaar ah oo la hagaajiyay iyo kuleelka bilawga ah ee Taq enzyme waxay ka hortagi kartaa kordhinta aan gaarka ahayn iyo samaynta dimer-ka
■ Dareenka sare-wuxuu ogaan karaa nuqullo hoose oo template ah
■ Kala duwanaansho wanaagsan - oo ku habboon inta badan qalabka PCR ee tirada dhabta ah
Codsiga qalabka
falanqaynta qPCR
Socodka shaqada
Garaaf
Kaydinta iyo nolosha shelf
Xirmadan waa in lagu kaydiyaa meel ka fog iftiinka waana in lagu kaydiyaa heerkulka -20℃.Haddii si joogto ah loo isticmaalo, waxa kale oo lagu kaydin karaa 4℃ wakhti gaaban (10 maalmood).
