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  • Lnc-RT Heroᵀᴹ I (oo leh gDNase)

    Lnc-RT Heroᵀᴹ I (oo leh gDNase)

    Super Premix-ka-koowaad ee cDNA-ku-samaynta lncRNA

    Cat.No.RTL-09098/09099

    Degdeg ah oo xasaasi u ah hab-ku-noqosho gadaal ah oo ka soo saarida cDNA-da koowaad ee lncRNA.

    Ka saar gDNA 2 daqiiqo gudahood.

    15 daqiiqo si aad u dhamaystirto isku xidhka cDNA-gii ugu horeeyay

    Nidaamka qoraalka dib u celinta dareenka sare

    Deganaanshaha kulaylka sare, heerkulka falcelinta ugu fiicani waa 42 ℃, walina waxay leedahay wax qabad wanaagsan oo qoraal ah 50 ℃.

    weydiintafatiran
  • RT Easyᵀᴹ I Master Premix-ka ugu horreeya ee cDNA-yada

    RT Easyᵀᴹ I Master Premix-ka ugu horreeya ee cDNA-yada

    -Nidaamka dib-u-dejinta oo hufan, kaliya waxay qaadataa 25 daqiiqo in la dhammaystiro isku-darka cDNA-ga ugu horreeya.

    - Nidaamka qoraalka dib u celinta dareenka sare, jaantusyada heerka pg sidoo kale waxay heli karaan cDNA tayo sare leh.

    - Nidaamka qoraalka dib-u-celinta wuxuu leeyahay xasillooni heerkul sare ah, heerkulka falcelinta nidaamku wuxuu noqon karaa mid sarreeya 50 ℃,waxayna leedahay wax qabad wanaagsan oo qoraal ah.

    - Xataa muunadaha RNA ee aan nadiifka ahayn (khamriga ilaa 45%, cusbada guanidine ilaa 750mM) ayaa lagu soo rogi karaa falcelin qoraal ah.

    xoog hor leh

    weydiintafatiran
  • RT Easy II (oo leh gDNase) Master Premix oo loogu talagalay isku-dhafka cDNA-ga ugu horreeya ee PCR ee leh gDNase

    RT Easy II (oo leh gDNase) Master Premix oo loogu talagalay isku-dhafka cDNA-ga ugu horreeya ee PCR ee leh gDNase

    -Awood waxtar leh oo looga saarayo gDNA, taas oo ka saari karta gDNA qaab-dhismeedka 2 daqiiqo gudahood.

    -Nidaamka dib-u-dejinta oo hufan, waxay qaadataa 15 daqiiqo oo keliya in la dhammaystiro isku-darka cDNA-ga ugu horreeya.

    Hababka isku dhafan: qaab-dhismeedka ka kooban GC-ga sare iyo qaab-dhismeedka sare ee adag ayaa sidoo kale lagu beddeli karaa hufnaan sare.

    -Nidaamka qoraalka dib u celinta dareenka sare, jaantusyada heerka pg sidoo kale waxay heli karaan cDNA tayo sare leh.

    -Nidaamka qoraalka dib u celinta ayaa leh xasilooni kulayl sare leh, heerkulka falcelinta ugu fiican waa 42℃, oo wali waxay leedahay wax qabad wanaagsan oo qoraal ah oo 50 ah℃.

    xoog hor leh

    weydiintafatiran
  • RT Easyᵀᴹ II Master Premix oo loogu talagalay isku-darka cDNA-ga ugu horreeya ee PCR-da

    RT Easyᵀᴹ II Master Premix oo loogu talagalay isku-darka cDNA-ga ugu horreeya ee PCR-da

    -Nidaamka dib-u-dejinta oo hufan, waxay qaadataa 20 daqiiqo oo keliya in la dhammaystiro isku-darka cDNA-ga ugu horreeya.

    - Nidaamka qoraalka dib u celinta dareenka sare, jaantusyada heerka pg sidoo kale waxay heli karaan cDNA tayo sare leh.

    - Nidaamka qoraalka dib u celinta wuxuu leeyahay xasillooni kuleyl sare leh, heerkulka falcelinta nidaamku wuxuu noqon karaa mid aad u sarreeya 50 ℃, wuxuuna leeyahay waxqabad qoraal ah oo wanaagsan.

    - Xataa muunadaha RNA ee aan nadiifka ahayn (khamriga ilaa 60%, cusbada guanidine ilaa 750mM) ayaa lagu soo rogi karaa falcelin qoraal ah.

    - 2× RT AMA-fududTM Isku-darku wuxuu ku daray qormooyin-qoraal oo la hagaajiyay (Random Primer, Oligo(dT)18 Hordhac).

    xoog hor leh

    weydiintafatiran
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