Waa intee 7 daqiiqo?Waa 420 ilbiriqsi.
Maxaan ku qaban karaa 7 daqiiqo gudahood?Waa koob shaah ah, oo hees la dhegaysanayo;waa farxadda dhex marta fasalka, laakiin welwelka ka hor inta aan daaha ka bixin.
Hadda wixii ka dambeeya, Foregene waxay siisay muhiimad cusub:
7 daqiiqo = Buuxi saxan dhan RNA diyaarinta saxan 96-ceel ah!
Looma baahna in la ururiyo unugyada, godka saxanka ee goobta kala goynta, kuleejka oo dhan heerkulka qolka!
lysis unug & saareDNA-da genomic
Jooji falcelinta
Fudud oo degdeg ah, hufnaan✖96!Waxay kaa caawinaysaa inaad si dhakhso leh oo hufan u dhammaystirto shuruudaha ogaanshaha heerka muujinta RNA ee unugyada dhaqameed, sida baadhista daroogada iyo habaynta muujinta, si weyn u dhiirrigeliso horumarka mashruuca, oo si hufan u dhammaystir hawsha!
Alaabta laxidhiidha
Unugyada si toos ah ayaa loo nadiifiyaa si ay u sii daayaan RNA ee RT-qPCR;nidaamka dulqaadka sare wuxuu ka dhigayaa mid aan loo baahnayn in la nadiifiyo RNA oo si toos ah loo isticmaalo lysates-ka unugga sida qaababka RNA ee falcelinta RT.Degdeg ah oo ku habboon;dareen sare, gaar gaar ah oo xoog leh, iyo xasilooni wanaagsan.
Degdega fududTM Qalabka tooska ah ee RT QPCR Kit-Taqmaan
Unugyada Tooska ah ee Lysis Unug u diyaarsan Hal-Tallaabo Qalabka QRT-PCR Baadhista
Degdega fududTM Qalabka Tooska ah ee Unugga RT QPCR-SYBR GREEN I
Unugyada Tooska ah ee Lysis unug u diyaarsan Hal-Tallaabo QRT-PCR Xirmooyinka
Waqtiga post: Dec-06-2022
