Qaybaha iyo Warshada Taxane ee Go'doominta DNA |Soosaarayaasha taxanaha go'doominta DNA ee Shiinaha

Qalabka Go'doominta DNA-ga Dhirta Xirmooyinka Nadaafadda DNA-da ee Genomic Plant Protocol

Cat.No.DE-06111/06112/06113

Sifeynta DNA-da genomic ee unugyada dhirta kala duwan.

Si dhakhso leh u nadiifi oo hel DNA-ga tayada sare leh ee shaybaarka dhirta (oo ay ku jiraan polysaccharides iyo muunadaha dhirta polyphenol).

◮Wax wasakhaysan RNase ma jiro

◮Xawaaraha dheereeya

◮Fudud: Hawlgalka sifaynta waxa lagu dhamayn karaa 30 daqiiqo.

◮Ku habboonHeerkulka qolka, 4℃ centrifugation iyo ethanol roobaadka DNA looma baahna.

◮Badbaadada: lama isticmaalo reagent organic.

weydiin tafatiran
Xirmooyinka Go'doominta DNA Nudaha Xayawaanka Xirmooyinka Soo-saarista DNA ee Muunadaha Unugyada Xoolaha

Soo saarista degdega ah iyo nadiifinta DNA-da genomic ee ilo badan, sida unugyada xayawaanka, unugyada, iwm.

◮Xawaaraha dheereeya:Dhaqdhaqaaq ka sarreeya borotiinka la midka ah oo si dhakhso ah u dheefshiido shaybaarada unugyada;

◮Fudud:waxa lagu dhamayn karaa 50 daqiiqo.

◮Ku habboon:Lagu sameeyay heerkulka qolka.

◮Badbaadada:looma isticmaalo reagent organic.

weydiin tafatiran
Qalabka yar ee DNA-ga

Si dhakhso leh uga nadiifi DNA-ga genomic ee tayada sare leh dhiiga xinjirowga lidka ku ah (<1ml).

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

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Xirmooyinka Yaryar ee Plasmid-ka Guud ee Plasmid-ka Soo saaridda Miniprep Mini Kits

Si dhakhso leh uga nadiifi DNA-da plasmid ee tayada sare leh bakteeriyada la beddelay ee tijaabooyinka bayoolojiga joogtada ah sida isbeddelka iyo dheefshiidka ensaymka.

◮Ka saar RNA adigoon ku darin RNase

◮Ku habboon-centrifugation-ka waxaa lagu sameeyaa heerkulka qolka

◮Degdeg ah-qaliinka waxa lagu dhamayn karaa 20 daqiiqo

◮Ammaan- lama isticmaalo reagent organic

◮daahirnimo sare-OD260/280≈1.7-1.9

weydiin tafatiran
Qalabka Midi-ga ee DNA-da Dhiiga

Si dhakhso leh uga nadiifi DNA-ga genomic ee tayada sare leh dhiiga xinjirowga lidka ku ah (1-5ml).

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

weydiin tafatiran
Qalabka Go'doominta ee Kaarka FTA ee Kaarka DNA-ga Soosaarida ama Xirmada Sifaynta

Si dhakhso leh uga nadiifi DNA-ga genomiga tayada sare leh ee swab buccal/Samballada Kaarka FTA

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

weydiin tafatiran
Genomic DNA Micro Kit Micro Genomic DNA Mini Kit

Si dhakhso leh u nadiifi muunado yaryar si aad u hesho DNA-ga genomic oo tayo sare leh.

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

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Xirmada Go'doominta DNA-da ee aan unugga lahayn

Ka soocida cfDNA ee shaybaarada balaasmaha

Lambarka buugga TQ01BT0050, TQ01BT0100

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Xirmada Go'doominta DNA ee Biyaha Xirmada Soosaarida iyo Sifaynta DNA ee Biyaha

Si deg deg ah uga nadiifi DNA-ga genomic-ga tayada sare leh ee ka yimaadda microorganisms ee muunado biyo oo kala duwan.

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

weydiin tafatiran
Xirmada Go'doominta DNA ee saxarada Xirmooyinka Ka-soo-saarka DNA ee saxarada

Si dhakhso leh u nadiifi oo hel DNA-ga hidde-ahaaneed oo tayo sare leh oo laga qaado muunado saxaro kala duwan.

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

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Qalabka Nadiifinta PCR Nidaamka Nadiifinta PCR ee DNA-da saafiga ah

Si dhakhso leh u nadiifi oo u hel jajabyada DNA-da tayada sare leh ee nidaamka PCR;Haddii aad rabto inaad kala tagto oo aad hesho jajab DNA oo gaar ah, fadlan dooro xirmada soo kabashada jel.

◮ Soo kabashada DNA-da oo ballaadhan: Qaybaha DNA-da oo gaaban sida 60bp iyo inta 10kb ah waa la soo ceshan karaa.

◮ Waxtarka soo kabashada sareWaxtarka soo kabashada guud ahaan waa in ka badan 80%, kan ugu sarreeyana wuxuu gaari karaa in ka badan 95%.

Xawaaraha dheereeya:Fudud in la shaqeeyo, soo kabashada jajabka DNA-da waxaa lagu dhameyn karaa 15 daqiiqo gudahood iyo dimer-ka si toos ah ayaa loo saari karaa iyada oo aan dib u soo kabashada jel.

◮ SafeefSoo saarista reagent organic looma baahna.

◮ Tayo sare: Qaybaha DNA-da ee la helay waa nadiif sare, kuwaas oo la kulmi kara tijaabooyin kala duwan oo xiga.

weydiin tafatiran
Xirmada Go'doominta DNA ee FFPE Qalabka Sifaynta DNA ee Ka Soo Bixinta Tusaalooyinka FFPE

Si dhakhso leh uga nadiifi DNA-ga genomic-ga tayada sare leh unugyo ku-xidhan paraffin-ka sida qaybaha paraffin iyo blocks paraffin.

◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.

◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.

◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.

◮Badbaadada:Looma baahna soo saarista reagent organic.

◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.

◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.

weydiin tafatiran