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RT Easyᵀᴹ I Master Premix-ka ugu horreeya ee cDNA-yada
-Nidaamka dib-u-dejinta oo hufan, kaliya waxay qaadataa 25 daqiiqo in la dhammaystiro isku-darka cDNA-ga ugu horreeya.
- Nidaamka qoraalka dib u celinta dareenka sare, jaantusyada heerka pg sidoo kale waxay heli karaan cDNA tayo sare leh.
- Nidaamka qoraalka dib-u-celinta wuxuu leeyahay xasillooni heerkul sare ah, heerkulka falcelinta nidaamku wuxuu noqon karaa mid sarreeya 50 ℃,waxayna leedahay wax qabad wanaagsan oo qoraal ah.
- Xataa muunadaha RNA ee aan nadiifka ahayn (khamriga ilaa 45%, cusbada guanidine ilaa 750mM) ayaa lagu soo rogi karaa falcelin qoraal ah.
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