Qorayaasha: Wang Xiaoyan, Zhao Eryu

Unug: Isbitaalka Jiaozhou, Isbitaalka Dongfang ee ku xiran Jaamacadda Tongji

Waqtigan xaadirka ah, nooca ugu weyn ee muunadda ugu weyn ee ogaanshaha bakteeriyada nucleic acid waa cunaha suufka.Waxa jira noocyo badan oo ah xalalka ilaalinta muunada ee sida caadiga ah loo isticmaalo, kuwaas oo dhamaantood u baahan in la qaboojiyo ama lagu qaboojiyo gaadiidka iyo kaydinta;Way adag tahay in la xakameeyo dhammaan habka heerkulka hooseeya inta lagu jiro habka ururinta iyo gaadiidka, wayna adagtahay in la hubiyo ilaalinta tayada ka hor inta aan la tijaabin saamiga.^[1-2].

RNase (RNase) waa endonuclease kaas oo hydrolyzes RNA, inta badan gooya bonds fosfodiester u dhexeeya nucleotide.Unugyada RNase waa mid aad u deggan, waxaa ku jira isku-xidhka disulfide ee qaab-dhismeedka, hawshiisana uma baahna joogitaanka cations divalent, sidaas darteed RNase si fudud uma furfurna, wayna fududahay in dib loo soo celiyo xitaa ka dib heerkulka sare ama isticmaalka denaturants.RNases waxay u qaybsan yihiin kuwo endogenous iyo kuwo dibadda ka baxsan.RNases-ka endogenous waxa la sii dayn karaa isla marka unugyadu dilaacaan.Sidaa darteed, baabi'inta doorka RNases endogenous waa tallaabo aad muhiim u ah habka soo saarista RNA.RNases dibadda ah ayaa si ballaaran loo qaybiyaa.RNases waxay ku jiraan hawada, maqaarka bini'aadamka, timaha iyo candhuufta, kuwaas oo ah sababaha muhiimka ah ee hoos u dhaca fudud ee RNA^[3].

Weydiinta xogta

CANS-CL02-A009 "Codsiga Tayada Shaybaarka Caafimaadka iyo Tilmaamaha Aqoonsiga Kartida ee Goobta Cilmi-baarista Molecular" ee shuruudaha farsamada waxay soo jeedinaysaa in heerarka tayada biyaha ee habboon loo qaabeeyey si waafaqsan codsiga;Weelka hawo-mareenka ah ee la tuuri karo:

RNase/ Kala saarid

(1) RNase A

Ribonuclease A (RNase A), oo ka soo jeeda beeryarada bovine, waa endoribonuclease kaas oo si gaar ah u weerari kara 3′ dhamaadka hadhaaga pyrimidine ee RNA, gooynta cytosine ama uracil oo ay sameeyeen nucleotide ku xiga.Bondka Fosfodiester, sheyga ugu dambeeya ee falcelinta waa 3′ pyrimidine nucleotide iyo oligonucleotide oo leh 3′ pyrimidine nucleotide dhamaadka.

(2) RNase T1

Ribonuclease T1 (RNase T1) waxaa laga soo qaatay Aspergillus orjzae, waxay si gaar ah u shaqeysaa 3′-terminal phosphate of guanine, meesha kala goysa waxay u dhexeysaa 3′ phosphate of guanine iyo 5′ hydroxyl ee nucleotide ku xiga.Shayga ugu dambeeya ee falcelinta waa 3′guanylic acid iyo jajabyada oligonucleotide oo leh 3′guanylic acid dhamaadka.

(3) RNase H

Ribonuclease H (RNase H) ayaa markii ugu horreysay laga helay unugyada kubka thymus, iyo hidde-sidaha codeynta ayaa lagu xiray Escherichia coli.Waxay si gaar ah hoos ugu dhigi kartaa DNA-da: xargaha RNA ee duplexes-ka isku-dhafka ah ee RNA, taasoo keentay oligonucleotides iyo mononucleotides oo leh 3'-OH iyo 5'-monophosphate, ma hoos u dhigi karto hal-ama laba-jibbaaran DNA ama RNA.

RNase

Shaqada iyo isticmaalka

Ribonuclease wuxuu kicin karaa nabaad-guurka ribonucleic acid (RNA) waxaana lagu soo saari karaa si macmal ah.Boomaatada daawada waxaa loo isticmaalaa si kor loogu qaado dhaawacyada iyo kalagoysyada xanuunka.Sida laga soo xigtay warbixinnada, ribonuclease waxay bedeli kartaa dheef-shiid kiimikaadka unugyada martida loo yahay, waxay joojin kartaa isku-darka fayraska, waxay joojinaysaa faafinta fayraska hargabka ee vitro, waxayna joojinaysaa samaynta tallaalka iyo fayraska herpes ee embriyaha digaagga.Isticmaalka kiliinikada ee ribonuclease maalin kasta cirbadeynta muruqa ee 180 mg, faa'iido u leh daaweynta encephalitis faafa, ribonuclease waxay kicin kartaa hoos u dhaca ribonucleic acid (RNA), oo hadda waa la farsameyn karaa.Boomaatada daawada waxaa loo isticmaalaa si kor loogu qaado dhaawacyada iyo kalagoysyada xanuunka.Sida laga soo xigtay warbixinnada, ribonuclease waxay bedeli kartaa dheef-shiid kiimikaadka unugyada martida loo yahay, waxay joojin kartaa isku-darka fayraska, waxay joojinaysaa faafinta fayraska hargabka ee vitro, waxayna joojinaysaa samaynta tallaalka iyo fayraska herpes ee embriyaha digaagga.Isticmaalka kiliinikada ee ribonuclease maalin kasta cirbadeynta muruqa ee 180 mg ayaa faa'iido u leh daaweynta encephalitis faafa.

RNase

Qeexitaan ka hortag

Qeexida unugga inhibitor RNase: Qadarka enzyme loo baahan yahay si loo joojiyo 50% ee dhaqdhaqaaqa 5ng RNase A waa hal unug.

Sida Horjoogayaasha RNase u shaqeeyaan

Guanidine isothiocyanate:

Guanidine isothiocyanate waa iskudhis organic ah oo leh caanaha kelli C2H6N4S.Inta badan loo isticmaalo dawooyinka noolaha, reagents kiimikaad, iwm. Guanidine isothiocyanate waa wakiilka lysing borotiinka, waxaana inta badan loo isticmaalaa sida qaybta ugu muhiimsan ee xalka lysis ee reagents ogaanshaha molecular.Waxay lyse kartaa nudaha, burburin kartaa qaab-dhismeedka unugyada iyo kala soocida nucleic acid ee nucleoprotein, oo waxay leedahay denaturation xoog leh RNase.Waa xakameynta RNase ee ugu waxtarka badan hadda.

TRIzol waa sheeko cusub wadarta guud ee soo saarista RNA oo si toos ah uga soo saari karta wadarta guud ee RNA unugyo ama unugyo.Waxa ku jira maaddooyin ay ka mid yihiin phenol iyo guanidine isothiocyanate, kuwaas oo si degdeg ah u carqaladayn kara unugyada oo joojin kara nukleysyada ay sii daayaan unugyada.

(Si kastaba ha ahaatee, guanidine isothiocyanate waxay halis u tahay caafimaadka cilmi-baarayaasha shaybaarka.)

RNasin:

Acid glycoprotein oo laga soo saaro beerka jiirka ama blastoderm aadanaha.Rnasin waa xannibaadaha aan tartanka ku jirin ee RNase, kaas oo ku xidhi kara RNases kala duwan si ay u kiciyaan.

(Faahfaahin dheeraad ah:https://www.foreivd.com/foreasy-rnase-inhibitor-product/)

Hheerkulka hoose:

Heerkulka sare sidoo kale waa habka caadiga ah ee denaturation borotiinka.

Diethylpirocarbonate (DEPC):

DEPC waa ka hortag xoog badan laakiin aan dhamaystirnayn RNase, kaas oo joojin kara dhaqdhaqaaqa RNase iyada oo la isku darayo amino acid imidazole giraanta kooxda RNase ee firfircoonida borotiinnada.

Vanadyl ribonucleoside complex:

Qalab ay sameeyeen ion vanadium oxide iyo nucleosides, kuwaas oo ku xidhan RNase qaab walxaha kala-guurka ah, kuwaas oo si buuxda u joojin kara dhaqdhaqaaqa RNase.

mid kale:

SDS, urea, diatomaceous earth, iwm. ayaa sidoo kale saameyn xaddidan ku leh RNase.

Falanqaynta Khubarada

Farsamada sare ee Li Yujie

Agaasimaha Waaxda Shaybaadhka, Isbitaalka Jiaozhou, Isbitaalka Dongfang ee ku xiran Jaamacadda Tongji

Si looga hortago burburka noole ee RNase dibadda ah, waji-gashiga, galoofyada iyo koofiyadaha waa in la gashado oo la beddelaa si joogto ah inta lagu jiro habka soo saarista RNA.Dhammaan weelasha dhalada ah waa in lagu dubaa foornada lagu qalajiyo 200 ° C in ka badan 2 saacadood.Alaabta loo isticmaalo dubista, sida bacaha, waxay u baahan yihiin in lagu daaweeyo biyaha DEPC, ka dibna lagu dhaqo biyo nadiif ah.Reagen-yaasha ama aaladaha loo isticmaalo soo saarista, ilaalinta iyo aqoonsiga RNA waa in loo go'aamiyaa RNA, waana in la dejiyaa aag hawlgalka RNA madax banaan.

tixraacyo:

[1] Smith-Vaughan HC, Binks MJ, Beissbarth J, iyo al.Bakteeriyada iyo fayrasyada ku jira nasopharynx isla markiiba ka hor bilowga caabuqyada ba'an ee hoose ee neef-mareenka ee carruurta asaliga ah ee Australiya[J].Eur J Clin Microbiol Infect Dis,2018,37 (9): 1785-1794.

[2] Laanta Xakamaynta Caabuqa Cisbitaalka ee Ururka Daawada Ka Hortagga ee Shiinaha.Tilmaamaha ururinta iyo baadhista shaybaarada microbial kiliinikada [J].Wargeyska Shiinaha ee Caabuqa Cisbitaalka, 2018 (20): 3192-3200.

[3] "Tijaabada Kiliiniga Toban Kun Sababta Mugga Tijaabada Biology-ga Molecular"