In ka badan kala badh 2022, kaalay oo arag waxa maqaallada dhibcaha sare leh uu soo dhejiyay Foeregene!
01
HADDII: 17.694
Waxaa daabacay: Isbitaalka First Affiliated, Jaamacadda Sun Yat-sen
Waxyaabaha ugu muhiimsan: Kansarka unugyada squamous unuggu (ESCC) waa nooca hoose ee kansarka hunguriga.Aqoonsiga bartilmaameedyada daawaynta ee waxtarka leh ayaa muhiimad weyn u leh daawaynta wanaagsan ee ESCC.Daraasadaha qaarkood waxay muujiyeen in khaldanaanta RNA ay kor u qaadi karto muujinta iyo tarjumaadda mRNA-yada la xidhiidha unugyada kansarka, laakiin habka molecular weli ma cadda.Daraasadani waxay muujisay in tRNA m7G methyltransferase borotiinada isku dhafan ee METTL1 iyo WDR4 ay si weyn u kordhiyeen unugyada ESCC waxayna gacan ka geysteen saadaasha liidata ee ESCC.Tan iyo markaas, METTL1 iyo WDR4-la xiriira daraasadaha garaacista hidda-wadaha ee jiirarka moodada ESCC ayaa xaqiijiyay in waxqabadka la xoojiyay ee tRNA m7G methyltransferase ay horseedayso hagaajinta muujinta labadan borotiin, taas oo iyaduna kor u qaadaysa faafitaanka unugyada ESCC.Daraasadani waxay muujinaysaa shaqada muhiimka ah ee oncogenic ee tRNA m7G wax ka beddelka ESCC waxayna soo jeedinaysaa in beegsiga METTL1 iyo dariiqyadeeda calaamadaynta hoose laga yaabo inay ballanqaadaan bartilmaameedyada daaweynta ee daaweynta ESCC.
Alaabta la caawiyay:
Mouse Tail Direct PCR Kit Foregene#TP-01333
Xiriirinta asalka ah:
https://www.nature.com/articles/s41467-022-29125-7
02
HADDII: 14.903
Waxaa daabacay: Shaybaarka Muhiimka ah ee Gobolka ee Cilmi-baarista Tayada ee Daawada Shiinaha, Machadka Sayniska Caafimaadka Shiinaha, Jaamacadda Macau
Nuxurka ugu muhiimsan:
Horumarinta tignoolajiyada synthetic, mRNA waxay noqotay nooc cusub oo dawooyinka daweynta ah.Si kastaba ha noqotee, gaaritaanka bixinta waxtarka leh ee unugyada unugyada ee dawooyinka mRNA ayaa weli ah mid adag sababtoo ah dareenka mRNA ee RNases.Daraasadani waxay soo jeedinaysaa qorshe ku habboon bixinta nanostructures-ku-xidhan polyphenol kaas oo si hufan u bixin kara IL-10 mRNA gudaha vivo.Ka dib markii la isticmaalo nanostructure-kan si loo bixiyo daawooyinka mRNA, heerarka IL-10 ayaa si gaar ah loo habeeyay, si wax ku ool ah u xakameynaya muujinta arrimaha caabuqa, kor u qaadista dayactirka xuubka xuubka, ilaalinta unugyada epithelial colon ee apoptosis, iyo joojinta dextran sodium sulfate-ka-soo-baxa ba'an iyo yar yar ee murine colitis waxay leedahay saameyn weyn oo daaweyn ah oo ku saabsan bogsashada caabuqa.
Alaabta la caawiyay:
Wadarta Guud ee Xayawaanka Qalabka Go'doominta RNA ee Foregene#RE-03011
Xiriirinta asalka ah:
03
HADDII: 12.499
Waxaa daabacay: Shaybaarka Qaabka Cudurrada Xoolaha ee Tijaabada ah, Kulliyadda Caafimaadka Xoolaha, Jaamacadda Beeraha ee Sichuan
Nuxurka ugu muhiimsan:
Cadaadiska Oxidative ee shahwada ayaa ah qodobka ugu weyn ee horseedaya dhalmo la'aanta ragga, sidaas darteed waa ikhtiyaar macquul ah oo wax ku ool ah in la dhiso moodooyinka xayawaanka ee dhalmo la'aanta labka ah iyadoo la hagaajinayo nidaamka antioxidant si loo beegsado.Si kastaba ha ahaatee, hababka antioxidant-ka ee unugyada jeermiska labka ah lama faahfaahin.Daraasadani waxay xaqiijisay in hidda-wadaha LanCL1 uu yahay hidde-sidaha antioxidant-ka ee gaarka ah ee unugyada jeermiska labka ah, iyo muujintiisa waa la dhimay ama lala xiriiriyaa dhalmo la'aanta ragga ee aadanaha.Jiirarka labka ah ee ay ku yar yihiin hidda-wadaha LanCL1 waxay muujinayaan waxyeellada shahwada ogsaydhka ah iyo bacriminta liidata.Habka kala-soocidda shahwada unugyada jeermiska, mar haddii u janjeera oksaydhisku dhacdo, borotiinka-soo-saarka-gaar ah ee 1 (SP1) ayaa nidaamin doona muujinta hidda-wadaha LanCL1, taas oo joojinaysa habka oksaydhka ee shahwada.Daraasadani waxay muujinaysaa in dariiqa SP1-LanCL1 uu door muhiim ah ka ciyaaro nidaaminta homeostasis xiniinyaha iyo bacriminta lab ah oo lagu dhexdhexaadiyo dheelitirka redox, waxayna soo jeedinaysaa in jiirarka LanCL1-la beddelay ay leeyihiin codsiyo soo jiidasho leh sida qaabka xayawaanka ee dhalmo la'aanta ragga.
Alaabta Caawinta:
Mouse Tail DNA Mini Kit Foregene#DE-05211
Xiriirinta asalka ah:
http://europepmc.org/article/MED/35469022
◮Wakhti-qaadasho iyo sifeyn qaali ah looma baahna.
◮ Dalabka muunada waa yar yahay, kaliya qaado hal abuur.
◮Daawayn gaar ah sida shiidi iyo burburin looma baahna, qalliinkuna waa sahlan yahay.
◮Nidaamka PCR ee la wanaajiyey waxa uu awood u siinayaa PCR in uu yeesho gaar gaar ah oo adag iyo dulqaad adag oo loogu talagalay horjoogayaasha falcelinta PCR.
Wadarta Xayawaanka Qalabka Go'doonka RNA
◮ Looma baahna in laga walwalo xaalufka RNA.Nidaamka oo dhan waa RNase-free.
◮Si wax ku ool ah uga saar DNA-da adiga oo isticmaalaya Tiirka Nadiifinta DNA.
Ka saar DNA adiga oo aan ku darin DNA-da.
Fudud-dhammaan hawlgallada waxaa lagu dhammeeyaa heerkulka qolka.
◮Dhakhso—qalliinka waxa lagu dhamayn karaa 30 daqiiqo gudahood.
◮Safe — aan la isticmaalin reagent organic.
◮ daahirnimo sare—OD260/280≈1.8-2.1.
◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.
◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.
◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.
◮Badbaadada:Looma baahna soo saarista reagent organic.
◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.
Waqtiga post: Seb-13-2022
