Dhismaha Nidaamka SOP

Samee tijaabada PCR SOP si loo habeeyo habdhaqanka shaqaalaha tijaabada ah.

Tijaabayaashu waxay si adag u hoggaansamaan nidaamka qalliinka, oo ay yareeyaan wasakhda PCR ee laga yaabo inay sababaan arrimaha bani'aadamka ama ka hortagga dhacdada wasakhowga ee hawlgalka.Intaa waxaa dheer, tijaabiyaha waa in uu leeyahay aqoonta xirfadeed ee u dhiganta iyo xirfadaha, oo ay ku jiraan aqoonta ku shaqeynta qalabka la xidhiidha, caddaynta habka shaqada oo dhan, maaraynta hababka daaweynta ee wasakheynta iyo hababka xakamaynta tayada shaybaarka, iyo inuu awood u leeyahay inuu si sax ah u tarjumo natiijooyinka imtixaanka.

Dhismaha Heerka PCR Shaybaadhka

Shaybaadhka PCR wuxuu u qaybsan yahay afar qaybood mabda' ahaan, kuwaas oo kala ah aagga diyaarinta reagent, aagga muunad samaynta, aagga cabbirka, iyo aagga falanqaynta alaabta.Labada goobood ee hore waa aagagga kor-u-qaadista, labada aag ee ugu dambeeyana waa aagagga kor-u-qaadista.Aagga ka-hor-u-qaadista iyo aagga-kordhinta kaddib waa in si adag loo kala saaraa.Qalabka tijaabada ah, reagents, waraaqaha wax lagu duubo, qalimaan, alaabta nadiifinta, iwm waxay kaliya ka qulquli karaan aagga hore-u-qaadista ilaa aagga-weyneynta ka dib, taas oo ah, laga bilaabo aagga diyaarinta reagent, aagga farsamaynta muunada, aagga kor u qaadista, iyo aagga falanqaynta badeecada, waana inaan u qulqulin jihada dambe.Socodka hawada ee shaybaarka waa inuu sidoo kale ka soo qulqulaa aagga hore-u-qaadista ilaa aagga cod-weyneeye ka dambeeya, maaha jihada gadaale.

Iska yaree talaabooyinka tijaabada ah

Haddii shaybaarku kaliya sameeyo ogaanshaha PCR iyo aqoonsiga, waxaa lagu talinayaa in la isticmaalo PCR tireed fluorescent halkii PCR caadiga ah.

Natiijooyinka ogaanshaha tirada PCR ee fluorescence waxaa lagu soo ururin karaa oo lagu falanqeyn karaa calaamadaha fluorescent, markaa ma jirto baahi loo qabo in la furo daboolka electrophoresis ka dib falcelinta, taas oo ka fogaanaysa faddaraynta alaabta PCR ee ay sababtay daadinta alaabta falcelinta si ay u sameeyaan aerosols.Haddii aad kordhiso tirada furayaasha furka inta lagu jiro tallaabada rarka ee jel electrophoresis, wasakhowga aerosol waxay u badan tahay inay dhacdo.Waxaa lagu talinayaa in la horumariyo codsiga PCR tirada iyo si tartiib ah u beddelo PCR tayo leh.

Qaado nidaamka ka hortagga wasakhowga ee UNG

Habka wasakheynta alaabta UNG anti-PCR waxaa loo isticmaalaa falcelinta PCR.

Nidaamku wuxuu isticmaalaa dUTP halkii uu ka isticmaali lahaa dTTP.Ka dib falcelinta PCR, dhammaan alaabada PCR (qaybaha DNA) waxaa lagu daray dUTP;wareegga xiga ee falcelinta PCR, enzyme UNG ee lagu daray nidaamka waxaa lagu beeray 37°C 5 daqiiqo kahor PCR, taas oo noqon karta mid gaar ah Hoos u dhig dhammaan jajabyada DNA-da ee ay ku jiraan dUTP, ka dibna samee falcelinta PCR.Tani waxay gebi ahaanba meesha ka saari kartaa wasakhowga hawada ee ay sababaan alaabta PCR.Saamaynta ayaa lagu muujiyay sawirka hoose:

Fiiro gaar ah: Taxanaha PCR ee tooska ah, waxaad dooran kartaa badeecadaha taxanaha ah ee nidaamka wasakhaynta alaabta PCR ee FJ Biotech

Soo jeedi

Shaybaadhyada sameeya baadhista genotyping-ga baaxadda leh, waxa aad loogu talinayaa in la isticmaalo nidaamka wasakhaynta alaabta UNG anti-PCR si loo tijaabiyo reage-yaasha marka lagu daro dhismaha shaybaadh macquul ah.

Xusuusin: Isticmaalka nidaamkan kama saari karo wasakhaynta alaabta PCR ee mar horeba sababtay.Sidaa darteed, nidaamka UNG waa in la adeegsadaa bilowga imtixaanka ku habboon, iyo nidaamka UNG waa in had iyo jeer loo adeegsadaa xoojinta PCR, si looga hortago wasakheynta alaabta PCR.Been togan.

Waxaa lagu talinayaa in la isticmaalo nidaamka tooska ah ee PCR-UNG ee Forge Biotech marka la samaynayo tijaabo baaxad leh, sida: Plant Leaf Direct PCR Kit-UNG;

Abuurka Dhirta ee tooska ah ee PCR Kit-UNG;

Kit-UNG ee Unugyada Xoolaha Tooska ah ee PCR;

Mouse Tail Direct PCR Kit-UNG;

Zebra Fish Direct PCR Kit-UNG.

Xirmooyinkan taxanaha ah ee Foregene ma samayn karaan oo keliya ogaanshaha PCR si dhakhso ah iyo miisaan ballaadhan, laakiin sidoo kale si wax ku ool ah uga hortagga iyo xakamaynta wasakhaynta alaabta PCR.