Insaymiska Taq-bilawga kuleyl ayaa si weyn loo isticmaalaa.Marka la barbar dhigo polymerase DNA-ga caadiga ah, kuleelka Taq enzyme wuxuu si wax ku ool ah uga fogaan karaa qaar ka mid ah kordhinta aan gaarka ahayn iyo samaynta dimers primer, waxayna si wax ku ool ah u wanaajin kartaa heerka guusha ee kordhinta hidda-wadaha.Gaar ahaan goobta baaritaanka hidda-socodka, bilawga kulul ee Taq enzyme ayaa loo aqoonsaday inuu yahay heerka qasabka ah ee warshadaha, iyo polymerase DNA-ga caadiga ah waa inaan la isticmaalin.Sida kor ku xusan, Taq enzymes-ka bilawga ah ayaa si weyn loo isticmaalaa.Waqtigan xaadirka ah, waxaa jira noocyo badan oo ah enzymes Taq-bilaaban kulul oo ku yaal suuqa gudaha, laakiin ma jiraan wax badan oo taq-bilawga-kuleyl ah oo tayo sare leh.Inaga oo wajahaya alaab badan oo bilow kulul Taq enzyme, sidee ayaan u dooranaa?

1. Dooro enzyme Taq-bilaw ah oo leh hufnaan sare

Hufnaanta PCR waxay si dhow ula xiriirtaa waxqabadka enzyme Taq.Ka dib marka nidaamka falcelinta enzyme Taq wanaagsan la hagaajiyo, hufnaanta kordhintu waxay ka sarraysaa 95%, iyo baaxadda kordhinta ee qaddarka template bilowga ah waa mid ballaaran.Kordhinta lagu qanco waxaa la heli karaa marka nuxurka hidde-sidaha bartilmaameedku uu hooseeyo, mana fududa in la sumeeyo marka qaddarka qaab-dhismeedku sarreeyo, iyo muddada jibbaarada-weynaynta ay dheer tahay.Enzyme Taq oo leh waxqabad liidata, xitaa haddii nidaamka falcelinta la hagaajiyay marar badan, waxtarka kor u qaadista ayaa weli ka yar 90%, qaabka "S" ee qalooca kor u qaadida ma aha mid muuqda, jiirarku waa yar yahay, qaloocana waa mid siman.Marka qadarka qaab-dhismeedku uu hooseeyo, lama kordhin karo, iyo marka qadarka qaab-dhismeedku sarreeyo, saamaynta kordhintu maaha mid ku habboon.Sidaa darteed, xulashada polymerases DNA oo leh hufnaan sare oo wax ku ool ah ayaa muhiim u ah guusha PCR iyo qPCR.

2. Dooro Ensaymes Taq-kuleyl leh oo leh awood enzyme xoog leh

Awoodda enzymatic ee enzyme Taq waxay la xiriirtaa waxtarka xoojinta.Guud ahaan, marka ay sii xoogaysato awoodda enzymatic-ku-kuleelka ee Taq enzyme, way dheeraysaa muddada koritaanka jibbaarada ee kordhinta PCR, qalooca 'S-qaabeeya' ee caadiga ah, ayaa sare u kacaya qiimaha calaamada fluorescence, iyo aad ugu habboon ogaanshaha PCR multix.Polymerases DNA-ga ee leh awoodda enzymatic daciifka ah waxay guud ahaan taageeri kartaa falcelinta 2-plex.Markaad samaynayso falcelin 3-plex ah, qalooca cod-weyneyntu waa hooseeyaa, qiimaha calaamadaha fluorescence waa hooseeyaa, mana jiro qalooca dhawaaqa caadiga ah, sidaas darteed natiijadu way adagtahay in la xukumo.

3. Dooro enzyme Taq-kuleyl ah oo leh dareen sare

Guud ahaan, DNA polymerase waxay leedahay wax ku oolnimada sare u qaadis iyo dareen sare, laakiin sidoo kale waxaa jira khilaafyo.Haddii tirada badan ee hidde-sidaha la beegsanayo ee muunadda la kordhinayo ay yar tahay, waxaa lagu talinayaa in la tijaabiyo dareenka xoojinta enzyme Taq.Habka ogaanshaha ugu caansan waa in la sameeyo 10-laab ama 5-laab dhuxusha jaangooyooyinka jajabka hidda-wadaha plasmid ee bartilmaameedka ah, la sameeyo ogaanshaha PCR ee qasinta hoose, oo dooro tan-kuleelka Taq enzyme oo leh dareen sare oo ogaansho.

Waxa kor laga arki karaa in cilmi-baarayaashu ay u baahan yihiin inay doortaan si waafaqsan shuruudaha tijaabada iyo shuruudaha maalgelinta.Way fiicantahay in la sameeyo tijaabada xoojinta milgaha gradient-ka si loo ogaado hufnaanta cod-weyneysiinta iyo dareenka bilowga-kuleelka ah ee Taq enzyme.

Tusaale ahaan Foregene's Taq DNA Polymerase:

Horudhac HS Taq DNA Polymerase

Sharaxaada

Foreasy HS Taq DNA Polymerase waa DNA polymerase lagu muujiyey bakteeriyada Escherichia coli ee injineernimada farsamada dib-u-habaynta hidda-wadaha.Insaymiska waxa lagu daraa falcelin gaar ah, kaas oo ka dhigaya badeecada mid aad u adkaysi badan oo la jaan qaadi karta, waxayna si toos ah u isticmaali kartaa muunada lysate-ka (Foregene Lysis system) oo tusaale u ah falcelinta ogaanshaha.

Codsiga

PCR-ta tayada leh iyo ogaanshaha PCR-ga tirada ee qaababka la safeeyey iyo qaab-dhismeedka aan la sifayn.

Xakamaynta tayada

1.Lama arag wax dhaq-dhaqaaq ah oo nucleus ah

Habka 2.PCR si loo ogaado hadhaaga genomic DNA iyada oo aan marti loo ahayn

3.Waxay si wax ku ool ah u kordhin kartaa hidde-sideyaasha koobi-kaliya ee Genome-ka aadanaha

4. Ku kaydi heerkulka qolka muddo toddobaad ah, dhaqdhaqaaqa muuqda ayaa isbeddelaya

Faahfaahinta alaabta: https://www.foreivd.com/foreasy-hs-taq-dna-polymerase-product/