Inta aad taqaan

Ku saabsan Nsoo saarista ucleic acid

Bilawga iyo dhammaadka aashitada nucleic ee la safeeyey

Wax walba way adag yihiin bilowga, aashitada nucleic ee la safeeyey ayaa ugu badan

Bilawga tijaabooyinka molecular, ee tijaabooyinka xiga

Guusha ama guuldaradu waxay leedahay saamayn muhiim ah.

Raadinta / ultra-trace UV spectrophotometer waxaa door biday cilmi-baarayaal badan oo saynis ah sababtoo ah waxay ogaan kartaa xoojinta acid nucleic iyo borotiinka iyo hadhaaga milixda si fudud, dhaqso iyo dhaqaale.

Sida aan wada ognahay, A260 macnaheedu waa nuugista acid nucleic OD qiimaha, A280 macnaheedu waa nuugista fiirsashada borotiinka qiimaha OD, A230 macnaheedu waa nuugista ion cusbada qiimaha OD, sidaa darteed A260 waxay u rogi kartaa xoojinta aashitada nucleic, A260/280 macnaheedu waa hadhaaga borotiinka, A260/230 macnaheedu waa hadhaaga milixda, laakiin tani waa tiro badan oo natiijooyin ku saleysan cilmi baaris.caadiga ah"si loo rumaysto inay u sharxi karto nadiifnimada DNA iyo RNAxad gaar ah.Ma aha halbeegga kaliya ee lagu aqoonsanayo wax-soo-saarka aashitada nukliyeerka iyo nadiifnimada, waxaa loo isticmaalaa oo kaliya tixraac ahaan, mana aha "heerka dahabka".

Buugga Thermo Fisher ND-2000 wuxuu xooga saarayaa isku halaynta natiijooyinka baaritaanka

Sababta ayaa ah in natiijooyinka la helay iyadoo la adeegsanayo micro / ultramicro UV spectrophotometer kaliya waxay ka tarjumaysaa dhalidda iyo nadiifnimada nucleic acid dhinaca, waxaana jira waxyaabo badan oo saameynaya (dariiqa indhaha, mugga muunada, fiirsashada saamiga, qiimaha pH, ions kale oo saameeya cabbirka nuugista, iwm.) , Qiimaha OD ee la qiyaasay maahan mid sax ah.Isla mar ahaantaana, sababtoo ah la'aanta gaarka ah ee alaabta go'aaminta qiimaha nuugista, DNA-da hal-xidhaale, DNA-da laba-geesoodka ah, RNA, dNTPs, iyo borotiinada qaarkood waxay leeyihiin ugu sarreeya nuugista 260nm mawjadaha, iyo diiradda ay go'aamiso A260 oo keliya maahan inay tahay DNA-da dhabta ah ama RNA.Xoog-saarid, iyo hufnaanteeda iyo hoos-u-dhaceeda lama qiimayn karo.

Haddaba sidee loo xukumayaa tayada aashitada nukleic ee la safeeyey?Marka lagu daro isticmaalka micro / ultramicro UV spectrophotometer si loo ogaado, hababka sida agarose gel electrophoresis ayaa sidoo kale looga baahan yahay si muuqaal ah loo muujiyo nadiifnimada iyo daacadnimada nucleic acid, iyo in la muujiyo feejignaanta ilaa xad.Sidan oo kale, wax-soo-saarka aashitada nucleic-ka iyo nadiifinta laga helay natiijooyinka ogaanshaha habab kala duwan ayaa lagu kalsoonaan karaa.

Isbarbardhigga shaxda tijaabada

DNA-da genomic ee unugyada H1299 ayaa la soo saaray iyada oo la adeegsanayo qalabyada soo saarista DNA ee shirkadda A, waxaana la arkay wasakhayn weyn oo wasakhaysan, taasoo keentay qiyam sare oo OD ah.

(Qiimaha cabbirka OD iyo electropherogram u dhigma)

Tusmada qiimaynta

Ma jiraa "heerka dahabka" ee wax-soo-saarka aashitada nuclei-ka iyo baaritaanka tayada?

Ilaa hadda sida aan ognahay, ma jiro hab fudud, degdeg ah oo jaban.Haddii ay tahay spectrophotometer, jel electrophoresis, ama spectrometry mass, dhammaantood waxay ka tarjumayaan xoojinta iyo nadiifnimada nucleic acid ee xalka dhinacyo kala duwan, waxayna u baahan yihiin in lagu xukumo midba midka kale.

Tusmada qiimaynta ugu fiican waa had iyo jeercodsiga tijaabada ah ee daba-galka ah.Ma saamaynayso hufnaanta qoraalka dib u noqoshada iyo kordhinta PCR.Helitaanka alaab-weyneyn la isku halayn karo iyo qiyamka Ct, iyo helitaanka taxane dhammaystiran iyadoo la isku xigxigo waa soo saarista aashitada nucleic-ka ah ee lagu guuleysto.

Si loo soo koobo, aragtida aragtida saamiga-keliya waa in lagu xujeeyaa aragtida "adeegsiga natiijooyinka tijaabada hoose ee wanaagsan ee cabbirrada soo saarista wanaagsan"!

Xirmooyinka soo saarista DNA-da Foregene ee lagula taliyay si loo helo nadiif sare oo DNA/RNA ah:

https://www.foreivd.com/reagent/dna-isolation-series/

https://www.foreivd.com/reagent/rna-isolation-series/