Hababka PCR iyo wasakhowga aashitada nucleic ee shaybaarada baarista aashitada nukliyeerka waa sida labada dhinac ee lacagta.Waxaan dooran karnaa oo kaliya inaan haysano iyo in kale, laakiin ma dooran karno inaan rabno ama aan kharash gareeyno.

1. Baaritaanka ka saarida DNA-ga

Si loo gaaro meesha ka saarida wasakheynta aashitada nucleic acid, marka hore waa lagama maarmaan in la baaro kuwa ka saaraya DNA-da kuwaas oo ka saari kara asiidhyada nucleic-ka ah xaalad dareere ah.Sababtoo ah ma jiraan wax badan oo ka saara DNA-da oo runtii shaqeeya.Habka tijaabada ah, fadlan tixraac: saarista DNA ma noqon karto "gees sir ah" shaybaarka!

Tijaabadan, 100 koobi/μL (CT ku dhawaad ​​31) ee ASFV plasmid iyo saarista DNA oo lagu qiyaaso PCR dhijitaalka ah ayaa la isku qasay si isku mid ah, ka dibna loogu falceliyay heerkulka qolka 10min, 20min iyo 30min siday u kala horreeyaan.Ka dib markii la soo saaro aashitada nucleic, qPCR ayaa la sameeyay.Xakameyn togan oo lagu daray plasmid iyo biyo ayaa la is barbar dhigay.Mar haddii tijaabadii aan la dhamaystirin isku mar, waxaa laga yaabaa in ay jirto leexleex gaar ah oo u dhexeeya natiijooyinka, laakiin ma saameynayso gabagabada tijaabada.Ilaa hadda, waxaan qiimeeyay 10 badeecooyin ah oo DNA-da ka saara.Kaliya No. 1, No. 6 iyo No. 8 alaabooyinka ayaa si wax ku ool ah hoos ugu dhigi kara plasmid DNA ee xaalad dareere ah.Alaabooyinka kale wax saameyn ah kuma laha.

Shaxda 1 Saamaynta ka saarista aashitada nukliyeerka ee ka saarida DNA-da ganacsi ahaan la heli karo

2. Tijaabada saamaynta saarista DNA-da ee jeermiska jeermiska ee sida caadiga ah loo isticmaalo

1. Baaritaanka jeermiska jeermiska dila ee sida caadiga ah loo isticmaalo

Waxaa jira noocyo badan oo jeermis dileyaal ah oo caadi ahaan loo isticmaalo shaybaarada: aldehydes, phenols, alcohols, quaternary ammonium salts, peroxides, diyaarinta koloriin, iyo acids iyo bases.Saamaynta jeermis-dilaha ee jeermiskan jeermiska ee noolaha waxaa si buuxda loogu xaqiijiyay tijaabooyin, laakiin ma jiraan xog tijaabo ah oo ku filan oo ku saabsan saamaynta nabaad-guurka ee asiidhyada nucleic-ka.Shaybaarada tijaabinta aashitada nukliyeerka waxay u baahan yihiin jeermis-dile kaas oo aan kaliya ku yeelanayn jeermiska jeermiska wanaagsan ee noolaha, laakiin sidoo kale hoos u dhigi kara DNA-da.Natiijooyinka tijaabada ayaa muujinaya in kaliya laba diyaarsan oo koloriin ah oo ah 84 jeermis dileyaal ah iyo trichloroisocyanuric acid iyo 1 M hydrochloric acid ay buuxiyeen shuruudaha.Maadaama 1 M hydrochloric acid uu aad u daxaleeyo, waxaa lagu talinayaa in diyaargarowga koloriin loo isticmaalo qashin-qubka shaybaarada baarista aashitada nukliyeerka.Si kastaba ha ahaatee, diyaarinta koloriintu waa daxalaysa biraha oo looma isticmaali karo jeermi-dilista qalabka iyo qalabka.

Shaxda 2 Saamaynta ka saarista DNA ee noocyada kala duwan ee jeermiska

2. U-fiirsashada ugu yar ee waxtarka leh ee diyaarinta chlorine

jeermis-dileyaasha chlorine-ku-saleysan waxay leeyihiin saameyn xoog leh oo hoos u dhaca DNA-da, laakiin daxalka birta iyo xanaaqa awgeed, waxaa loo isticmaali karaa in lagu nadiifiyo sagxadda, miisaska aan birta ahayn, talooyinka qoynta, tuubooyinka centrifuge iyo walxaha kale ee baaritaanka.

Marka loo eego "Hagaha Farsamo Farsamo ee Jeermiska Cudurrada Wadnaha ee Cusub": Wasakhowga (dhiigga bukaanka, dheecaanka iyo matagga) koloriin la heli karo 5g/L-10g/L jeermiska-koolorayn-ku jira;sagxadaha, gidaarada iyo sagxadaha walaxda waxay isticmaalaan koloriin la heli karo 1g/L koloriin jeermis dilaha: dharka, gogosha iyo dunta kale ayaa marka hore lagu qooyay jeermiska koloriinta iyadoo 0.5g/L koloriin diyaar ah 30 daqiiqo, ka dibna la nadiifiyaa sidii caadiga ahayd.

Shaxda 3 Saamaynta ka saarista DNA-da ee uruurinta kala duwan ee jeermiska koloriinta

Natiijooyinka imtixaanku waxay muujinayaan in: marka fiirsashada koloriin ee waxtarka leh ay ka weyn tahay ama la mid tahay 1.2 g/L, jeermiska ka kooban koloriinku wuxuu gebi ahaanba hoos u dhigi karaa 100 koobi/μL plasmid marka la isticmaalo 5 daqiiqo.Marka lagu dhaqmo 10 daqiiqo, 84 jeermis dile oo leh koloriin waxtar leh oo ka weyn ama la mid ah 0.6 g/L waxay si buuxda hoos u dhigi kartaa 100 koobi/μL ee plasmids.

3. Tijaabada hawada ka saarista oo ay wasakhaysay aerosolka nucleic acid

Sidee loo nadiifiyaa hawada wasakhaysan aerosols nucleic acid?Inta badan fikradaha waa in la abuuro jawi wasakhaysan hawada ka dibna la nadiifiyo, ama in la nadiifiyo asiidhka nucleic ka dib wasakhda.Aniga, tijaabo aan la soo celin karin oo natiijada tijaabada aan tiro ahaan lagu falanqeyn karin macno ma leh, sidaas darteed waxaan soo amaahday qaar ka mid ah hababka jeermiska-dilka hawada.Eeg "GB27948-2020 Shuruudaha Guud ee Jeeriyeyaasha Hawada" iyo "Qeexitaannada Farsamada jeermiska"

1. Qalabka tijaabada

1.1 saarista DNA: Tijaabadan, laba saarayaal DNA oo ganacsi ahaan loo heli karo 6 iyo 8 ee shaxda 1 ayaa la doortay.

1.2 Tijaabada plasmid: Iyadoo la tixgelinayo in qiimaha CT ee wasakhowga shaybaarka badankiisu uu ka sarreyn doono 30, xoojinta hidda-wadaha ASFV plasmid ee wakhtigan la isticmaalay waa qiyaastii 100 koobi/μL, iyo tirada PCR CT ee fluorescent waa qiyaastii 31.07.Ku dar balaasmid-ka suufka qulqulaya oo hawo engegan.

1.3 Qalabka Tijaabada: Qalabka jeermis-dilaaca ee Bilingkehan KVBOX, oo ay si naxariis leh u taageerto Shenzhen Runlian Environmental Technology Co., Ltd., waxay leedahay wax qabad nadiifinta hawada aad u wanaagsan oo leh jeermiska hydrogen peroxide, waxayna si wax ku ool ah u dili kartaa xadhkaha iyo noolaha kale.

1.4 Meesha tijaabada: daaqad wareejin ah oo xiran oo qiyaastii 0.1 mitir kuyuub ah.

2. Habka tijaabada

Qadarka jeermis-dilaha ee lagu rakibay mashiinka jeermiska waa 10ml, xoogasaarkuna waa 100ml/m3, taas oo aad uga badan "GB27948-2020 Shuruudaha Guud ee jeermiska Hawada": marka la isticmaalayo jeermiska buufinta aerosol, qadarka jeermiska waa inuu noqdaa ≤10 ml/m3