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Mouse Tail Direct PCR Qalabka
Qalabkani wuxuu adeegsanayaa nidaam kayd ah oo gaar ah oo loo yaqaan 'lysis buffer system' si dhakhso loogu sii daayo hidde-sideha hiddo-wadaha ah ee dabo, dhegaha, muruqyada iyo shaybaarrada kale ee unugyada ee fal-celinta PCR, sidaa darteed waxay si gaar ah ugu habboon tahay baaritaanka hidda-socodka ballaaran
Nidaamka lagu sii daayo hidde-sideha hiddo-wadaha ah ee dheecaanka ku jira ayaa lagu dhammaystiraa 10-30 daqiiqo gudahood 65 ° C. Majiro habab kale sida borotiinka iyo ka saarida RNA looma baahna, raad raaca la sii daayayna DNA ayaa loo isticmaali karaa tusaale ahaan ficil celinta PCR.
2 × M1-PCR Easy TM Mix wuxuu leeyahay dulqaad xoog leh oo ku saabsan ka hortagayaasha falcelinta PCR, wuxuuna isticmaali karaa lakabka muunadda si loogu tijaabiyo sheybaar loogu talagalay kordhinta waxtarka leh iyo tan gaarka ah. Reagent-kan wuxuu ka kooban yahay Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, jawaabta keydka, PCR optimizer iyo xasiliyaha.
D-Taq DNA polymerase waa DNA polymerase si gaar ah loogu sameeyay Foregene falcelinta tooska ah ee PCR. D-Taq DNA polymerase wuxuu leeyahay dulqaad xoog leh noocyada kala duwan ee ka hortagayaasha falcelinta PCR, wuxuuna si wax ku ool ah u kordhin karaa tirada raadraaca DNA ee nidaamyada kala duwan ee falcelinta isku dhafan, iyo xawaaraha ballaadhintu wuxuu gaari karaa 2Kb / min, kaas oo si gaar ah ugu habboon falcelinta tooska ah ee PCR.
