Xirmada Ashitada Nukliyeerka ah ee SARS-CoV-2 (Habka Baaritaanka Fluorescent PCR Multiplex)
Sharaxaada Qalabka:
PCR-ga tooska ah:Looma baahna xirmada soo saarista aashitada nucleic-ka iyo Nidaamka, oo la dhameeyay 96 muunado 1 saac ama wax ka badan.
◮ Qalabka loo baahan yahay oo hooseeya iyo isticmaalka aadka u dabacsan:Kaliya waxaad u baahan tahay nidaamka PCR-waqtiga dhabta ah.
◮ LoD iyo dareen sare:Dareenka ogaanshaha wuxuu gaari karaa heerka ugu hooseeya sida 500 nuqul/ml.
Isticmaalka la damacsan yahay
Xirmadani waxay isticmaashaa tignoolajiyada RT PCR ee-waqtiga-dhabta ah (rRT-PCR) si loo ogaado tayada SARS-CoV-2 asiidhyada nucleic-ka ah ee sanbabada sanka ee bini'aadamka ama muunadaha suufka oropharyngeal.
Mabda'a imtixaanka
Qalabkan aasaasiga ah iyo aaladaha baaritaanka waxaa loogu talagalay in lagu ogaado taxanaha la keydiyay ee SARS-CoV-2 (ORF1ab hiddo-wadaha iyo hiddaha N).Qalabka waxaa ku jira Kontorool Gudaha ah oo loo isticmaalo in lagu qiimeeyo tayada muunada.
Tignoolajiyada PCR-da-waqtiga-dhabta ah waxay isticmaashaa falcelinta silsiladda polymerase (PCR) si loo kordhiyo yoolka gaarka ah
taxane iyo bartilmaameedyo baadhisyo gaar ah oo lagu ogaanayo RNA la xoojiyay.Baarayaasha waxaa lagu calaamadeeyay
wariyaha fluorescent iyo dheeha.
Faahfaahin Deg Deg ah
◮Tilmaamaha:48 Rxns/kit, 96 Rxns/kit
Gaar ahaan:96.72%
Dareenka97.92%
◮ Noocyada muunada ee lagu dabaqi karo
Nasopharyngeal Swab ama Oropharyngeal Swab
◮Qalabka lagu dabaqi karo
ABI7500, Bio-Rad CFX96, Roche LightCycler 480, SLAN-96S
Qaybaha
| Maya | Qayb | Qadarka | Qaybaha ugu muhiimsan | |||
| 48 Rxns | 96 Rxns | |||||
| 1 | Wakiilka sii daaya acid nucleic | 1.4ml/tubo | 2 tuubbo | 5.3 ml/ dhalo | 1 dhalo | Surfactant |
| 2 | difaaca RNA | 27 μL/tubo | 1 tube | 53 μL/tubo | 1 tube | RNase inhibitor |
| 3 | Xalka falcelinta SARS-CoV-2 | 800 μL/tubo | 1 tube | 1600 μL/tubo | 1 tube | Fure, baadhitaan, falcelin celin, dNTP |
| 4 | Isku dhafka enzyme SARS-CoV-2 | 80 μL/tubo | 1 tube | 160 μL/tubo | 1 tube | Bilawga kulul ee Taq enzyme, M-MLV enzyme |
| 5 | SARS-CoV-2 xakamaynta togan | 100 μL/tubo | 1 tube | 100 μL/tubo | 1 tube | Plasmid dib-u-habayn oo ka kooban jajab bartilmaameed, RNA |
| 6 | SARS-CoV-2 xakamaynta taban | 1200 μL/tubo | 1 tube | 1200 μL/tubo | 1 tube | TE baffer |
Tilmaamaha & faa'iidooyinka
◮PCR toos ah
Looma baahna xirmada soo saarista aashitada nucleic-ka iyo Nidaamka, oo la dhameeyay 96 muunado 1 saac ama wax ka badan.
◮Qalabka loo baahan yahay oo hooseeya iyo isticmaalka aadka u dabacsan
Kaliya u baahan nidaamka PCR-Waqtiga dhabta ah.
◮LoD iyo dareen sare
Dareenka ogaanshaha wuxuu gaari karaa heerka ugu hooseeya sida 500 nuqul/ml.
Sida loo isticmaalo
Habka 1: Habka tooska ah ee PCR
Habka 2: Go'doominta Viral RNA + PCR
Habka 1: Habka tooska ah ee PCR adoo isticmaalayaTusaalaha Wakiilka Siideynta
Habka ogaanshaha
Gaadiidka Silsiladda Qabow iyo Kaydinta
Laga xidhay iftiinka oo lagu kaydiyay -20 ± 5 ℃;
Nolosha shelf
1 sano
Dalbashada Macluumaadka
Fiidiyowga Hawlaha Degdegga ah
Halkan ku qor fariintaada oo noo soo dir
