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Cadka Xayawaanka Xirmada Qalabka PCR
Qalabkani wuxuu isticmaalaa nidaam kayd ah oo loo yaqaan 'lysis buffer system' si dhakhso leh looga sii daayo hidde-sideha DNA-da shaybaarka unugyada xoolaha ee falcelinta PCR, markaa waxay si gaar ah ugu habboon tahay baaritaanka hidda-socodka ee ballaaran.
Nidaamka lagu sii daayo hidde-sideha hidde-wadaha ee dheecaanka lysis ayaa lagu dhammeeyaa 10-30 daqiiqo gudahood 65°C. Majiro waxqabadyo kale sida borotiinka iyo saarista RNA, iyo raadadka la sii daayay ee DNA ayaa loo isticmaali karaa tusaale ahaan falcelinta PCR.
2× PCR fududTM Isku-darka wuxuu leeyahay dulqaad xoog leh oo ku saabsan ka-hortagayaasha fal-celinta PCR, waxayna u isticmaali kartaa lakabka muunadda in lagu tijaabiyo qaab-dhismeedka wax-ku-oolnimada iyo ballaarinta gaarka ah. Reagent-kan wuxuu ka kooban yahay Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, keydka falcelinta, hagaajiyaha PCR iyo xasiliyaha.
D-Taq DNA polymerase waa DNA polymerase si gaar ah loogu sameeyay Foregene falcelinta tooska ah ee PCR. D-Taq DNA polymerase wuxuu leeyahay dulqaad xoog leh noocyada kala duwan ee ka hortagayaasha falcelinta PCR, wuxuuna si wax ku ool ah u kordhin karaa tirada raadraaca DNA ee nidaamyada kala duwan ee falcelinta isku dhafan, iyo xawaaraha ballaadhintu wuxuu gaari karaa 2Kb / min, kaas oo si gaar ah ugu habboon falcelinta tooska ah ee PCR.
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Mouse Tail SuperDirect Qalabka PCR
Qalabkani wuxuu isticmaalaa nidaam kayd ah oo loo yaqaan 'lysis buffer system' si dhakhso leh loogu sii daayo hiddo-wadaha hiddo-wadaha ah ee 'DNA genus' laga soo bilaabo jiirka jiirka iyo shaybaarka dhegaha jiirka ee fal-celinta PCR, markaa waxay si gaar ah ugu habboon tahay baaritaanka hidde-ballaadhan
Nidaamka sii deynta hidde-sidaha DNA-da ee ka soo baxa lys-ka ayaa la dhammaystiray 10-30 daqiiqo gudahood heerkulka qolka (20-25 ° C). Kuleyl looma baahna, hababka kale sida borotiinka iyo saarista RNA looma baahna. Qiyaasaha raadraaca la sii daayay ee DNA ayaa loo isticmaali karaa sidii Template loogu talagalay fal-celinta PCR.
2 × M-PCR Easy TM Mix wuxuu leeyahay dulqaad xoogan oo ku saabsan ka hortagayaasha falcelinta PCR, wuxuuna isticmaali karaa lakabka muunadda si loogu tijaabiyo sheybaar si wax ku ool ah oo loo kordhiyo. Reagent-kan wuxuu ka kooban yahay Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, jawaabta keydka, PCR optimizer iyo xasiliyaha.
D-Taq DNA polymerase waa DNA polymerase si gaar ah loogu sameeyay Foregene falcelinta tooska ah ee PCR. D-Taq DNA polymerase wuxuu leeyahay dulqaad xoog leh noocyada kala duwan ee ka hortagayaasha falcelinta PCR, wuxuuna si wax ku ool ah u kordhin karaa tirada raadraaca DNA ee nidaamyada kala duwan ee falcelinta isku dhafan, iyo xawaaraha ballaadhintu wuxuu gaari karaa 2Kb / min, kaas oo si gaar ah ugu habboon falcelinta tooska ah ee PCR.
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Cadka Animal Direct kit PCR kit-UNG
Qalabkani wuxuu isticmaalaa nidaam kayd ah oo loo yaqaan 'lysis buffer system' si dhakhso leh looga sii daayo hidde-sideha DNA-da shaybaarka unugyada xoolaha ee falcelinta PCR, markaa waxay si gaar ah ugu habboon tahay baaritaanka hidda-socodka ee ballaaran.
Nidaamka lagu sii daayo hidde-sideha hidde-wadaha ee dheecaanka lysis ayaa lagu dhammeeyaa 10-30 daqiiqo gudahood 65°C. Majiro waxqabadyo kale sida borotiinka iyo saarista RNA, iyo raadadka la sii daayay ee DNA ayaa loo isticmaali karaa tusaale ahaan falcelinta PCR.
2×PCR fududTM Isku-darka (UNG) wuxuu adeegsadaa dUTP halkii uu ka isticmaali lahaa dTTP asalka 2×PCR fududTM Isku qas, oo ku dar enzyme UNG (Uracil-N-glycosylase) oo hoos u dhigi kara shaybaarka ay ku jirto dUTP isla waqtigaas. Kahor falcelinta PCR, enzyme-ka UNG waxaa loo isticmaalaa in lagu duleeyo badeecada PCR ee uracil ku jiro. UNG enzyme wax saameyn ah kuma yeelan doono shaxanka aan ku jirin uracil, taas oo hubineysa qeexnaanta iyo saxsanaanta kordhinta iyo ka hortagga suurtagalnimada in alaabada PCR ay sumowdo inta lagu jiro baaritaanka hidaha ee ballaaran.
D-Taq DNA polymerase waa DNA polymerase si gaar ah loogu sameeyay Foregene falcelinta tooska ah ee PCR. D-Taq DNA polymerase wuxuu leeyahay dulqaad xoog leh noocyada kala duwan ee ka hortagayaasha falcelinta PCR, wuxuuna si wax ku ool ah u kordhin karaa tirada raadraaca DNA ee nidaamyada kala duwan ee falcelinta isku dhafan, iyo xawaaraha ballaadhintu wuxuu gaari karaa 2Kb / min, kaas oo si gaar ah ugu habboon falcelinta tooska ah ee PCR.
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Mouse Tail Direct PCR Qalabka
Qalabkani wuxuu adeegsanayaa nidaam kayd ah oo gaar ah oo loo yaqaan 'lysis buffer system' si dhakhso loogu sii daayo hidde-sideha hiddo-wadaha ah ee dabo, dhegaha, muruqyada iyo shaybaarrada kale ee unugyada ee fal-celinta PCR, sidaa darteed waxay si gaar ah ugu habboon tahay baaritaanka hidda-socodka ballaaran
Nidaamka lagu sii daayo hidde-sideha hiddo-wadaha ah ee dheecaanka ku jira ayaa lagu dhammaystiraa 10-30 daqiiqo gudahood 65 ° C. Majiro habab kale sida borotiinka iyo ka saarida RNA looma baahna, raad raaca la sii daayayna DNA ayaa loo isticmaali karaa tusaale ahaan ficil celinta PCR.
2 × M1-PCR Easy TM Mix wuxuu leeyahay dulqaad xoog leh oo ku saabsan ka hortagayaasha falcelinta PCR, wuxuuna isticmaali karaa lakabka muunadda si loogu tijaabiyo sheybaar loogu talagalay kordhinta waxtarka leh iyo tan gaarka ah. Reagent-kan wuxuu ka kooban yahay Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, jawaabta keydka, PCR optimizer iyo xasiliyaha.
D-Taq DNA polymerase waa DNA polymerase si gaar ah loogu sameeyay Foregene falcelinta tooska ah ee PCR. D-Taq DNA polymerase wuxuu leeyahay dulqaad xoog leh noocyada kala duwan ee ka hortagayaasha falcelinta PCR, wuxuuna si wax ku ool ah u kordhin karaa tirada raadraaca DNA ee nidaamyada kala duwan ee falcelinta isku dhafan, iyo xawaaraha ballaadhintu wuxuu gaari karaa 2Kb / min, kaas oo si gaar ah ugu habboon falcelinta tooska ah ee PCR.
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Mouse Tail Direct PCR Kit-UNG
Qalabkani wuxuu adeegsanayaa nidaam kayd ah oo gaar ah oo loo yaqaan 'lysis buffer system' si dhakhso loogu sii daayo hidde-sideha hiddo-wadaha ah ee dabo, dhegaha, muruqyada iyo shaybaarrada kale ee unugyada ee fal-celinta PCR, sidaa darteed waxay si gaar ah ugu habboon tahay baaritaanka hidda-socodka ballaaran
Nidaamka lagu sii daayo hidde-sideha hiddo-wadaha ah ee dheecaanka ku jira ayaa lagu dhammaystiraa 10-30 daqiiqo gudahood 65 ° C. Majiro habab kale sida borotiinka iyo ka saarida RNA looma baahna, raad raaca la sii daayayna DNA ayaa loo isticmaali karaa tusaale ahaan ficil celinta PCR.
2 × M1-PCR Easy TM Mix (UNG) waxay isticmaashaa dUTP halkii laga isticmaali lahaa dTTP iyadoo lagu saleynayo 2 × M1-PCR Easy TM Mix (UNG), waxayna ku dareysaa UNG enzyme (Uracil-N-glycosylase) oo hoos u dhigi kara shaybaarka ay ku jirto dUTP. isla waqtigaas. Kahor falcelinta PCR, enzyme-ka UNG waxaa loo isticmaalaa in lagu duleeyo badeecada PCR ee uracil ku jiro. UNG enzyme wax saameyn ah kuma yeelan doono shaxanka aan kujirin uracil, taas oo hubinaysa qeexida iyo saxsanaanta kordhinta iyo kahortaga suurtagalnimada baaritaanka hidaha ee baaxada weyn. Wasakheynta alaabada PCR ayaa dhacday.
D-Taq DNA polymerase waa DNA polymerase si gaar ah loogu sameeyay Foregene falcelinta tooska ah ee PCR. D-Taq DNA polymerase wuxuu leeyahay dulqaad xoog leh noocyada kala duwan ee ka hortagayaasha falcelinta PCR, wuxuuna si wax ku ool ah u kordhin karaa tirada raadraaca DNA ee nidaamyada kala duwan ee falcelinta isku dhafan, iyo xawaaraha ballaadhintu wuxuu gaari karaa 2Kb / min, kaas oo si gaar ah ugu habboon falcelinta tooska ah ee PCR.
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Qalabka loo yaqaan 'Zebra Fish Direct PCR Kit'
Qalabkani wuxuu isticmaalaa nidaam kayd ah oo loo yaqaan 'lysis buffer system' si dhakhso leh looga sii daayo hiddo-wadaha hiddo-wadaha ah ee loo yaqaan 'zebrafish' iyo unugyada kale ee kalluunka biyaha saafiga ah, baalasha dabada ama shay-baarka ukunta kalluunka ee fal-celinta PCR, markaa waxay si gaar ah ugu habboon tahay baaritaanka hidde-ballaadhan. Nidaamka lagu sii daayo hidde-sideha hiddo-wadaha ah ee dheecaanka ku jira ayaa lagu dhammaystiraa 10-30 daqiiqo gudahood 65 ° C. Majiro habab kale sida borotiinka iyo ka saarida RNA looma baahna, raad raaca la sii daayayna DNA ayaa loo isticmaali karaa tusaale ahaan ficil celinta PCR.
2 × PCR Easy TM Mix wuxuu leeyahay dulqaad xoogan oo ku saabsan ka hortagayaasha falcelinta PCR, wuxuuna isticmaali karaa lakabka muunadda si loogu tijaabiyo sheybaar loogu talagalay xoojinta iyo kordhinta qaaska ah. Reagent-kan wuxuu ka kooban yahay ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, jawaabta keydinta, PCR optimizer iyo xasiliyaha. Waxaa loo adeegsadaa keydka leysku xirayo si dhakhso leh oo fudud ayaa loo ogaan karaa shaybaarka, wuxuuna leeyahay astaamaha xasaasiyad sare, gaar ahaaneed adag, iyo xasillooni wanaagsan.
D-Taq DNA polymerase waa DNA polymerase si gaar ah loogu sameeyay Foregene falcelinta tooska ah ee PCR. D-Taq DNA polymerase wuxuu leeyahay dulqaad xoog leh noocyada kala duwan ee ka hortagayaasha falcelinta PCR, wuxuuna si wax ku ool ah u kordhin karaa tirada raadraaca DNA ee nidaamyada kala duwan ee falcelinta isku dhafan, iyo xawaaraha ballaadhintu wuxuu gaari karaa 2Kb / min, kaas oo si gaar ah ugu habboon falcelinta tooska ah ee PCR.
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Zebra Kalluunka Tooska ah PCR Kit-UNG
Qalabkani wuxuu isticmaalaa nidaam kayd ah oo loo yaqaan 'lysis buffer system' si dhakhso leh looga sii daayo hiddo-wadaha hiddo-wadaha ah ee loo yaqaan 'zebrafish' iyo unugyada kale ee kalluunka biyaha saafiga ah, baalasha dabada ama shay-baarka ukunta kalluunka ee fal-celinta PCR, markaa waxay si gaar ah ugu habboon tahay baaritaanka hidde-ballaadhan. Nidaamka lagu sii daayo hidde-sideha hiddo-wadaha ah ee dheecaanka ku jira ayaa lagu dhammaystiraa 10-30 daqiiqo gudahood 65 ° C. Majiro habab kale sida borotiinka iyo ka saarida RNA looma baahna, raad raaca la sii daayayna DNA ayaa loo isticmaali karaa tusaale ahaan ficil celinta PCR.
2 × PCR Easy TM Mix (UNG) waxay isticmaashaa dUTP halkii laga isticmaali lahaa dTTP iyadoo lagu saleynayo 2 × PCR Easy TM Mix, waxayna ku dareysaa UNG enzyme (Uracil-N-glycosylase) oo hoos u dhigi kara tusaalaha ay ku jirto dUTP isla waqtigaas. Kahor falcelinta PCR, enzyme-ka UNG waxaa loo isticmaalaa in lagu duleeyo badeecada PCR ee uracil ku jiro. UNG enzyme wax saameyn ah kuma yeelan doono shaxanka aan kujirin uracil, taas oo hubinaysa qeexida iyo saxsanaanta kordhinta iyo kahortaga suurtagalnimada baaritaanka hidaha ee baaxada weyn. Wasakheynta alaabada PCR ayaa dhacday.
D-Taq DNA polymerase waa DNA polymerase si gaar ah loogu sameeyay Foregene falcelinta tooska ah ee PCR. D-Taq DNA polymerase wuxuu leeyahay dulqaad xoog leh noocyada kala duwan ee ka hortagayaasha falcelinta PCR, wuxuuna si wax ku ool ah u kordhin karaa tirada raadraaca DNA ee nidaamyada kala duwan ee falcelinta isku dhafan, iyo xawaaraha ballaadhintu wuxuu gaari karaa 2Kb / min, kaas oo si gaar ah ugu habboon falcelinta tooska ah ee PCR.
