Saamiga hooseeya ee A260/A230 waxaa badanaa sababa wasakh leh dhererka dhererka nuugista ugu badan ee 230nm.Aan aragno waxa wasakhdaas ka mid ah:

• Wasakhowga caadiga ah	Mawjada dhererka nuugista	Saamaynta saamiga
  Protein	230nm iyo 280nm	Yaraynta isku mar ah ee A260/A 280iyo A260/A 280saamiga
  Guanidine cusbo	220-240 nm	Iska yaree A260/A 280saamiga
  Phenol	~ 270nm	-
  Trizol	230nm iyo 270nm	Iska yaree A260/A 280saamiga
  EDTA	~ 230nm	Iska yaree A260/A 280saamiga
  Ethanol	230-240 nm	Iska yaree A260/A 280saamiga

 
 
 
Mawjada dhererka nuugista iyo isbarbardhigga qiimaha wasakhowga caadiga ah

Pwasakhowga Rotein
Wasakhowga borotiinka waxaa loo arki karaa wasakhda ugu badan ee habka soo saarista aashitada nucleic.Borootiinku waxa uu u dhexeeyaa wajiga sare ee aqueous iyo kan hooseorganicwajiWasakhowga waxay hoos u dhigi doontaa saamiga A260/A280 iyo A260/A230 isku mar, saamiga A260/A230 si cad ayuu isu beddeli doonaa marka loo eego saamiga A260/A280.
Inta lagu guda jiro ku xigadib u qoristaor falcelinta qPCR, hadhaaga borotiinka waxay hor istaagi karaan ama faragalin karaan shaqada enzyme.Habka ugu fiican ee looga fogaado wasakhowga borotiinka waa in maskaxda lagu hayo mabda'a ah "halkii wax ka yar, qadar yar oo badan oo jeer ah" marka la rajaynayo supernatant.

2. Wasakhowga Guanidinium
hydrochloride (GuHCl) iyo guanidine thiocyanate (GTC) waxay leeyihiin saamaynta borotiinada diida, kuwaas oo si dhakhso ah u burburin kara xuubka unugyada inta lagu jiro habka soo saarista aashitada nucleic, taasoo keenta denaturation borotiinka iyo roobka.Mawjadaha nuugista ee GuHCl iyo GTC waa inta u dhaxaysa 220-240 nm, iyoCusbada guanidinium-ka hadhaa waxay yarayn doontaa saamiga A260/A230.Inkasta oo cusbada guanidinium-ka haraaga ah ay yarayn doonto saamiga,Saamaynta tijaabooyinka hoose ayaa dhab ahaantii dayacan.

3. faddaraynta Trizol
Qaybta ugu muhiimsan ee Trizol waa phenol.Shaqada ugu weyn ee phenol waa in ay lyse unugyada oo ay sii daayaan borotiinada iyo walxaha nucleic acid ee unugyada.Inkasta oo phenol uu si wax ku ool ah u diidi karo borotiinka, si buuxda uma joojin karo dhaqdhaqaaqa RNase.Sidaa darteed, 8 -hydroxyquinoline, guanidine isothiocyanate, β-mercaptoethanol, iwm ayaa lagu daraa TRIzol si loo xakameeyo RNase endogenous iyo exogenous.
Marka la soo saaro RNA-ga gacanta, Trizol waxay si dhakhso ah u lyse kartaa unugyada waxayna joojin kartaa nuklease laga sii daayo unugyada, iyo Trizol haraaga waxay si weyn u yareyn doontaa saamiga A260 / A230.
Habka habaynta: Marka centrifuging, waa in la ogaadaa in phenol ee Trizol si fudud u milmay in wajiga biyaha hoos xaaladda 4 ° iyo heerkulka qolka.

4. Haraaga ethanol
Ethanol waxaa loo isticmaalaa habka ugu dambeeya si loo soo dedejiyo DNA-da iyadoo la kala dirayo ions milix ah oo laga yaabo inay ku xiran yihiin DNA-da.Mawjada nuugista ee ugu sareysanuugista ugu sarreysaethanol sidoo kale waa 230-240 nm, kaas oosidoo kale waxay hoos u dhigi doontaa saamiga A260/A230.
Habka looga fogaado haraaga ethanol waxaa lagu soo celin karaa laba jeer inta lagu jiro elution kama dambaysta ah, afuufaya gudahaqiiqa qiiqamuddo laba daqiiqo ah si loogu oggolaado ethanolku inuu si buuxda u uumibaxo ka hor inta aan lagu darin kaydka elution.
Si kastaba ha ahaatee, waa in la ogaadaa in saamiga uu yahay kaliya qiimeynta qiimeynta tayada RNA.Haddii hawlgallada kor ku xusan si adag loo nidaamiyo, leexashada u dhaxaysa saamiga iyo qiyaasta caadiga ah saameyn weyn kuma yeelan doonto tijaabooyinka hoose.
Alaabooyinka La Xiriira:
Xirmada Go'doominta RNA Wadarta Xoolaha
Qalabka go'doominta ee RNA Total
Qalabka go'doominta unugga ee RNA
Qalabka Go'doominta Dhirta ee RNA Plus