Shiinaha oo si toos ah u soo saara aashitada nukliyeerka Soo saaraha iyo alaab-qeybiyaha |Foregene

si otomaatig ah u soo saarista nucleic acid

Soo saarista aashitada nukliyeerka ee tooska ah Sawirka

Sharaxaada Qalabka:

Qalabkani wuxuu isticmaalaa Real-time RT PCR technology (rRT-PCR) si loo ogaado tayada SARS-CoV-2 (ORF1ab hiddo iyo N gene) acids nucleic in nasopharyngeal bini'aadamka ama oropharyngeal swab muunado, by multiplex PCR fluorescent probe method. Tani xirmo ka kooban kontoroolka gudaha si loo qiimeeyo shaybaarka baahida shaybaarka ee tayada Nuclearka, sidaas darteed ma dhamaystiri karo baahida saafiga ah ee aashitada. ogaanshaha degdega ah.

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Waxaan raacnaa mabda'a maamulka ee "Tayadu waa mid gaar ah, Bixiye ayaa ugu sarreeya, Magaca ayaa ugu horreeya", oo si daacad ah u abuuri doona oo la wadaagi doona guusha dhammaan macaamiisha si otomaatig ah u soo saarista aashitada nukliyeerka, Waxaan aaminsanahay in adeegeena diirran oo xirfad leh uu kuu keeni doono yaabab wacan iyo sidoo kale nasiib.
Waxaan raacnaa mabda'a maamulka ee "Tayadu waa mid gaar ah, Bixiye ayaa ugu sarreeya, Magaca ayaa ugu horreeya", waxaanan si daacad ah u abuuri doonaa oo aan la wadaagi doonnaa guusha dhammaan macaamiishaShiinaha DNA/Rna Soo saarista Ashitada Nukliyeerka, Nidaamka Soo saarista Ashitada NukliyeerkaWaxaan helnay khibrad ku filan oo ku saabsan soo saarista alaabada iyadoo loo eegayo muunado ama sawiro.Waxaan si diiran u soo dhaweyneynaa macaamiisha ka yimid dalka iyo dibadda si ay u soo booqdaan shirkadeena, iyo in ay nagala shaqeeyaan mustaqbalka quruxda badan si wadajir ah.

Tilmaamaha

48 Rxns, 96 Rxns

Maya	Qayb	Qadarka				Qaybaha ugu muhiimsan
Maya	Qayb	48 Rxns		96 Rxns		Qaybaha ugu muhiimsan
1	Wakiilka sii daaya acid nucleic	1.4ml/tubo	2 tuubbo	5.3 ml/ dhalo	1 dhalo	Surfactant
2	difaaca RNA	27 μL/tubo	1 tube	53 μL/tubo	1 tube	RNase inhibitor
3	Xalka falcelinta SARS-CoV-2	800 μL/tubo	1 tube	1600 μL/tubo	1 tube	Fure, baadhitaan, falcelin celin, dNTP
4	Isku dhafka enzyme SARS-CoV-2	80 μL/tubo	1 tube	160 μL/tubo	1 tube	Bilawga kulul ee Taq enzyme, M-MLV enzyme
5	SARS-CoV-2 xakamaynta togan	100 μL/tubo	1 tube	100 μL/tubo	1 tube	Plasmid dib-u-habayn oo ka kooban jajab bartilmaameed, RNA
6	SARS-CoV-2 xakamaynta taban	1200 μL/tubo	1 tube	1200 μL/tubo	1 tube	TE baffer

Tilmaamaha & faa'iidooyinka

Degdeg ah oo fudud.
Iska ilaali luminta aashitada nucleic.
Xadka ogaanshaha hooseeya iyo dareenka sare.
Qalabka loo baahan yahay oo hooseeya iyo isticmaalka aadka u dabacsan.

Dareenka ogaanshaha wuxuu gaari karaa heerka ugu hooseeya sida 500 koobi/ml).

GCE/ml	Qiimaha Ct
GCE/ml	N hiddo-wadaha	ORF1ab hidde
2.00E+08	16.43	15.16
2.00E+07	19.48	18.58
2.00E+06	22.71	21.86
2.00E+05	25.95	25.33
2.00E+04	29.30	28.49
2.00E+03	32.37	31.37
2.00E+02	35.32	34.47
2.00E+01	38.29	38.27
2.00E+00	N/A	N/A
2.00E-01	N/A	N/A
NC	N/A	N/A

qalooca dhawaaqa toosan

qalooca xoojinta-RT-qPCR

Sida loo isticmaalo

1. Qalab lagu dabaqi karo

xirmada ogaanshaha covid-19 nucleic acid

2. Habka ogaanshaha

xirmada ogaanshaha covid-19 nucleic acid

Samaynta Tusaalaha: Muunada suufka naafeeya ama oropharyngeal (kaliya 1 tillaabo hawlgal) (Sug 10 daqiiqo)

PCR amplificaton (<= 55min)

Socodka shaqada:

xirmada ogaanshaha covid-19 nucleic acid 4

Kaydinta

laga xidhay iftiinka oo lagu kaydiyay -20 ± 5 ℃;

kaydinta
kaydinta2

Nolosha shelf:1 sano

Waxaan raacnaa mabda'a maamulka ee "Tayadu waa mid gaar ah, Bixiye ayaa ugu sarreeya, Magaca ayaa ugu horreeya", oo si daacad ah u abuuri doona oo la wadaagi doona guusha dhammaan macaamiisha PriceList for China Biobase PCR Laboratory DNA/Rna Nucleic Acid Extraction (Betsy), Waxaan aaminsanahay in adeegeena diirran iyo xirfad leh ay kuu keeni doonaan yaabab wacan iyo sidoo kale nasiib.
Liiska Qiimaha eeShiinaha DNA/Rna Soo saarista Ashitada Nukliyeerka, Nidaamka Soo saarista Ashitada NukliyeerkaWaxaan helnay khibrad ku filan oo ku saabsan soo saarista alaabada iyadoo loo eegayo muunado ama sawiro.Waxaan si diiran u soo dhaweyneynaa macaamiisha ka yimid dalka iyo dibadda si ay u soo booqdaan shirkadeena, iyo in ay nagala shaqeeyaan mustaqbalka quruxda badan si wadajir ah.

Hore: 2019 Shiinaha Naqshad Cusub oo Shiinaha La Qaadan karo Wakhtiga Dhabta ah Nidaamka Baadhitaanka PCR Isku-darka Soo Saaraya Ashitada Nukliyeerka Toos ah PCR Rna Nadiifinta

Xiga: Qiimo dhimis weyn oo Shiinaha PCR Tijaabada Reagent Real Time Medical Diagnostic Nucleic Acid Kit

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