Qalabka tooska ah ee PCR Plus caleenta (la'aanteed Qalabka Muunalada) borotokoolka tooska ah PCR ee agabka dhirta
Sharaxaada
Alaabtani waxay isticmaashaa nidaam u gaar ah si ay u liso caleemaha dhirta.lysate-ka waxaa loo isticmaali karaa qaab-dhismeedka iyada oo aan la nadiifin.Ka dib marka lagu daro aasaasayaasha, PCR Mix ee xirmadan ayaa loo isticmaali karaa xoojinta.
Tilmaamaha
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Qaybaha kitka
Qaybta I | Buffer PS1 |
Buffer P2 | |
6×Dejinta DNA-da | |
Qaybta II | Buffer PS2 |
2× Caleen PCR FududTMIsku qas |
Tilmaamaha & faa'iidooyinka
■ Ma jiro waqti-qaadasho iyo nadiifin DNA-da qaali ah
■ Waxyaabo yar
■ Fudud-muunada waxaa lagu soo rogi karaa falcelinta PCR ama falcelinta lysis iyada oo aan la jarin ama la shiidi
■ 2 × Isku qas, yaraynta khaladaadka muunada rarida iyo wakhtiga diyaarinta nidaamka falcelinta
■ Diyaarinta qaab-dhaqso leh ayaa lagu dhammeyn karaa 10 daqiiqo, falcelinta PCR waxay ku dhameyn kartaa ugu yaraan 50 daqiiqo
■ Dareen-celinta-lysis-ka sare ee fal-celinta waxaa lagu dhammayn karaa saxan PCR 96-wanaagsan
Xuduudaha Kit
Codsiga: Aqoonsiga dhirta transgenic, genotyping, iwm.
Tusaalaha: caleemaha dhirta qani ku ah polysaccharide ama polyphenol
Cabbirka Tusaalaha: Dhexroorka 2-3mm (habka tooska ah), dhexroorka 5-7mm (habka lysis)
Kala duwanaanshaha ogaanshaha: alaabta PCR ≤1kb