Horjoogaha RNase ee saadaasha ah
Sharaxaada Qalabka:
◮RNase inhibitor cusub oo jiirka ka soo jeeda ayaa lagu muujiyey bakteeriyada E. coli ee lagu farsameeyay iyadoo la isticmaalayo tignoolajiyada dib-u-habaynta hidda-wadaha.Kahortagtu waxay joojisaa dhaqdhaqaaqa RNase isagoo si aan tartan lahayn ugu daraya RNase 1:1, si uu u ilaaliyoRNAdaacadnimada , oo si wax ku ool ah u joojin karaahRNase A, B, C dhaqdhaqaaqa , laakiin ma aha RNase T1, T2, H, iwm. Waa la rogrogi karaa xidhitaanka RNase Inhibitor iyo RNase , iyo kakan waxaa lagu kala saari karaa urea iyo sulfhydryl reagents, si RNase dib-u-soo-noqoto iyo xakameyntu ay si aan leexleexad lahayn u firfircoonaato.
Tilmaamaha
Horyaalka RNase ee Foreasy waa jiir cusub oo ka soo jeeda RNase inhibitor oo lagu muujiyey bakteeriyada E. coli injineernimada iyadoo la adeegsanayo tignoolajiyada dib-u-habaynta hiddo-wadaha.Kahortagtu waxay joojisaa dhaqdhaqaaqa RNase isagoo si aan tartan lahayn ugu daraya RNase 1:1, si uu u ilaaliyoRNAdaacadnimada , oo si wax ku ool ah u joojin karaahRNase A, B, C dhaqdhaqaaqa , laakiin ma aha RNase T1, T2, H, iwm. Waa la rogrogi karaa xidhitaanka RNase Inhibitor iyo RNase , iyo kakan waxaa lagu kala saari karaa urea iyo sulfhydryl reagents, si RNase dib-u-soo-noqoto iyo xakameyntu ay si aan leexleexad lahayn u firfircoonaato.
Alaabtani waxay leedahay codsi la mid ah tan enzyme ka soo jeeda mandheerta aadanaha.Horyaalka RNase ee saadaasha ah wuxuu leeyahay heerkulbeeg sare.It waxa uu ilaaliyaa dhaqdhaqaaqa sare waxana uu joojiyaa RNase In habka qoraalka dib u noqoshada iyadoo la adeegsanayo heerkulbeeg heer sare ah oo beddelka qoraalka ah (sida: Foreasy Reverse Transcriptase).
Qaybaha kitka
| Qayb | IM-03011 | IM-03012 | IM-03013 |
| Horjoogaha RNase ee saadaasha ah (40 U/μL) | 50 KU (1.25 ml) | 200 KU (5ml) | 2000 KU (50 ml) |
Tilmaamaha & faa'iidooyinka
-HHeerkulbeegga.
-It waxa uu ilaaliyaa dhaqdhaqaaqa sare waxana uu joojiyaa RNase In habka qoraalka dib u noqoshada iyadoo la adeegsanayo heerkulbeeg heer sare ah oo beddelka qoraalka ah (sida: Foreasy Reverse Transcriptase).
Codsiga qalabka
Kahortagga RNase ee Foreasy waxaa loo isticmaali karaa dhammaan tijaabooyinka wasakheynta RNase ama faragelinta si RNA looga ilaaliyo hoos u dhaca;Tijaabooyinka, Hor-joogaha RNase ee Foreasy ma faragelin doono mana saameyn doono tijaabada lafteeda. Tusaale ahaan:
Isku-dubaridka xadhigga ugu horreeya ee cDNA, RT-PCR (RT-qPCR toos ah iyo RT-PCR toos ah)
In vitro qoraal/tarjumid
Joojinta dhaqdhaqaaqa RNase inta lagu jiro go'doominta RNA iyo nadiifinta
RNasetijaabada ilaalinta
Kaydinta iyo nolosha shelf
Ku kaydi at -20±5℃ 2 sano ama at -80℃ kaydinta muddada dheer.
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