Ku dheggan mabda'aaga "Tayada wanaagsan ee wanaagsan, adeegga lagu qanco", Waxaan ku dadaaleynaa inaan helno shirkad ganacsi oo fantastik ah adiga oo ah Qiimaha ugu Wanaagsan Shiinaha New Rapid Real Time Quantitative PCR Machine Price Test PCR ee iibka leh Shahaadada CE, Alaabooyin la sameeyay oo leh faa'iido calaamad ah.Waxaan si dhab ah uga qayb-galnaa si aan u soo saarno oo aan ugu dhaqanno si daacad ah, iyo raallinimada macaamiisha guriga iyo dibedda ee warshadaha xxx.
Ku dheggan mabda'aaga "Tayada wanaagsan ee wanaagsan, adeegga lagu qanco", waxaan ku dadaaleynaa inaan kuu helno lamaane ganacsi oo fantastik ahOgaanshaha Asiidh Nuclei ah oo Toos ah, Shiinaha Rt PCR Qalabka Ogaanshaha, Magaca Shirkadda, had iyo jeer waa la xiriira tayada sida aasaaska shirkadda, raadinta horumarinta via heerka sare ee kalsoonida , u hoggaansamaan heerka maamulka tayada ISO si adag, abuurista shirkadda sare-sare by ruuxa horumar-calaamad daacadnimo iyo yididiilo.

Qeexitaannada Qalabka

50 Diyaarin, 200 Diyaarin

Qalabkani wuxuu isticmaalaa tiirka lafdhabarta iyo caanaha ay soo saartay shirkadeena, kaas oo ka soo saari kara nadiif sare iyo tayada sare ee RNA laga soo bilaabo unugyo xayawaan ah oo kala duwan oo leh waxtar sare leh.Waxay bixisaa DNA-nadiifinta wax ku ool ah, kaas oo si sahlan u kala sooci kara oo u dhejin kara DNA-ga genomic-ka sare iyo unugyada lysate, fudud iyo waqti-badbaadinta;Tiirka RNA-keliya ayaa si hufan u xidhi kara RNA waxaana lagu farsamayn karaa qaacido gaar ah Tiro badan oo muunado ah.

Nidaamka oo dhan waa RNase-free, si RNA-ga la soo saaray aanu hoos u dhicin;Buffer RW1, Buffer RW2 nidaamka dhaqida kaydinta, si RNA-ga la helay uu xor uga ahaado borotiinka, DNA, ion, iyo wasakheynta isku dhafka dabiiciga ah.

Qaybaha qalabka:

Tilmaamaha & faa'iidooyinka

■ Looma baahna in laga walwalo xaalufka RNA;nidaamka oo dhan waa RNase-free
■ Si wax ku ool ah uga saar DNA-da adoo isticmaalaya Tiirka Nadiifinta DNA
■ Ka saar DNA adigoon ku darin DNA-da
■ Hawlgallada fudud-dhammaan waxaa lagu dhammeeyaa heerkulka qolka
■ Hawlgalka degdegga ah waxa lagu dhamayn karaa 30 daqiiqo
■ Badbaado-ma baahna reageent organic ah
■ Nadaafadda sare -OD260/280≈1.8-2.1

Codsiga qalabka

Waxay ku habboon tahay soo saarista iyo nadiifinta wadarta RNA ee noocyo kala duwan oo unugyo xayawaan ah oo cusub ama barafaysan ama unugyo dhaqan leh.

Xuduudaha alaabta

■ Codsiyada hoose: isku dhafka cDNA ee ugu horreeya, RT-PCR, cloning molecular, Northern Blot, iwm.
Tusaalooyinka: unugyada xoolaha, unugyo dhaqan
■ Qiyaasta: Unugyada 10-20mg, Unugyada (2-5)×10⁶
■ Awoodda ugu badan ee DNA-da ee tiirka sifaynta: 80 μg
■ Mugga iftiinka: 50-200 μl

Socodka shaqada

Jaantuska

Wadarta Xayawaanka Go'doominta RNA ee lagu daweeyay 20mg
Muuska cusub, qaado 5% wadarta guud ee RNA la safeeyey 1% agar

Glycogel electrophoresis
1: beeryarada 2: kelyaha
3: Beerka 4: Wadnaha

Kaydinta iyo nolosha shelf

Qalabka waxaa lagu kaydin karaa 24 bilood heerkulka qolka (15-25 ℃) ama 2-8 ℃ wakhti dheer.Buffer RL1 waxa lagu kaydin karaa 4 ℃ 1 bil kadib marka lagu daro β-mercaptoethanol (ikhtiyaar).

Ku dheggan mabda'aaga "Tayada wanaagsan ee wanaagsan, adeegga lagu qanco", Waxaan ku dadaaleynaa inaan helno shirkad ganacsi oo fantastik ah adiga oo ah Qiimaha ugu Wanaagsan Shiinaha New Rapid Real Time Quantitative PCR Machine Price Test PCR ee iibka leh Shahaadada CE, Alaabooyin la sameeyay oo leh faa'iido calaamad ah.Waxaan si dhab ah uga qayb-galnaa si aan u soo saarno oo aan ugu dhaqanno si daacad ah, iyo raallinimada macaamiisha guriga iyo dibedda ee warshadaha xxx.
Qiimaha ugu fiicanShiinaha Rt PCR Qalabka Ogaanshaha, Ogaanshaha Asiidh Nuclei ah oo Toos ah, Magaca Shirkadda, had iyo jeer waa la xiriira tayada sida aasaaska shirkadda, raadinta horumarinta via heerka sare ee kalsoonida , u hoggaansamaan heerka maamulka tayada ISO si adag, abuurista shirkadda sare-sare by ruuxa horumar-calaamad daacadnimo iyo yididiilo.