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Waxaan ognahay inaynu kobcin karno kaliya haddii aan dammaanad qaadno isku dhafka qiimaha tartanka iyo faa'iidada tayada sare leh isla waqti isku mid ahAaladda Tijaabada Degdegga ah ee Shiinaha, Imtixaanka PCr, Waxaan samaynaynay wax soo saarkayaga in ka badan 20 sano .Inta badan samee jumlo , marka waxaan leenahay qiimaha ugu tartanka badan , laakiin tayada ugu sareysa .Sanadihii la soo dhaafay , waxaan helnay jawaab celin aad u wanaagsan , ma aha oo kaliya sababtoo ah waxaan bixinaa badeecooyin wanaagsan , laakiin sidoo kale sababtoo ah adeegeena wanaagsan ee iibka kadib .Waxaan halkaan ku sugeynaa weydiintaada.

Tilmaamaha

200×20μl Rxns, 1000×20μl Rxns

Qaybaha kitka

Tilmaamaha & faa'iidooyinka

■ Fudud oo wax ku ool ah: tignoolajiyada Cell Direct RT, muunadaha RNA waxaa lagu heli karaa 7 daqiiqo oo keliya.

■ Baahida muunada waa yar tahay, maadaama ay yar tahay 10 unug ayaa la tijaabin karaa.

■ Soo saarista sare: waxay si dhakhso ah u ogaan kartaa RNA ee unugyada lagu beeray 384, 96, 24, 12, 6-cells.

■ Tirtiraha DNA-da si dhakhso ah ayuu u saari karaa genome-yada la sii daayay, si weyn ayuu u yareynayaa saameynta natiijooyinka tijaabada dambe.

■ Nidaamka RT iyo qPCR ee la hagaajiyay ayaa ka dhigaya laba-tallaabo dib-u-celinta RT-PCR mid hufan iyo PCR mid gaar ah, oo aad u adkeysi badan ka-hortagga falcelinta RT-qPCR.

Codsiga qalabka

Baaxadda codsiga: unugyada dhaqanka.

- RNA lagu sii daayay muunad lysis: kaliya lagu dabaqi karo qaabka RT-qPCR ee xirmadan.

-Qalabka waxa loo isticmaali karaa ujeedooyinka soo socda: falanqaynta muujinta hidde-sidaha, xaqiijinta saamaynta aamusinta hidde-sidaha ee siRNA, baadhista daroogada, iwm.

Jaantuska

Kaydinta iyo nolosha shelf

Qaybta I ee xirmadan waa in lagu kaydiyaa 4℃;Qaybta II waa in lagu kaydiyaa at -20 ℃.

Foregene Protease Plus II waa in lagu kaydiyaa 4 ℃, ha ku barafaysan -20 ℃.

Reagent 2× Toos ah qPCR Mix-SYBR waa in lagu kaydiyaa -20℃ gudcurka;haddii si joogto ah loo isticmaalo, waxa kale oo lagu kaydin karaa at 4 ℃ kaydinta muddada-gaaban (isticmaal ilaa 10 maalmood gudahood) .Waxaan ognahay in aan kaliya ku raaxaysan haddii aan dammaanad qaadi doono tartanka kharashka isku dhafan oo tayo sare leh waqti isku mid ah ee Best-Iibinta Shiinaha Antigen 1 Tijaabada degdegga ah Test Antigen Degdegga ah Test Strip Home Test for Single Isticmaalka , Waxaan si daacad ah u soo dhaweynayaa dibadda ee iibsada muddo dheer-in la tashado in muddo dheer la tashanaya.
Iibinta ugu WanaagsanAaladda Tijaabada Degdegga ah ee Shiinaha, PCR Test, Waxaan samaynaynay alaabtayada in ka badan 20 sano.Inta badan samee jumlo , marka waxaan leenahay qiimaha ugu tartanka badan , laakiin tayada ugu sareysa .Sanadihii la soo dhaafay , waxaan helnay jawaab celin aad u wanaagsan , ma aha oo kaliya sababtoo ah waxaan bixinaa badeecooyin wanaagsan , laakiin sidoo kale sababtoo ah adeegeena wanaagsan ee iibka kadib .Waxaan halkaan ku sugeynaa weydiintaada.