Waxaan ahayn soo saaraha khibrad leh.Ku guuleysiga inta badan shahaadooyinka muhiimka ah ee suuqeeda qiimo dhimista weyn ee Shiinaha Hal-Tallaabo Baaritaanka Sare ee Dareenka-QpcrKit V2, Tayada waa nolosha warshad, Diirada saar baahida macaamiisha waa isha badbaadada iyo horumarka shirkadda
Waxaan ahayn soo saaraha khibrad leh.Ku guuleysiga aqlabiyadda shahaadooyinka muhiimka ah ee suuqeedaShiinaha Taq DNA Polymerase, Qpcr, Shirkadeena waxay ballanqaadeysaa: qiimo macquul ah, waqti wax soo saar gaaban iyo adeegga iibka ka dib, waxaan sidoo kale kugu soo dhaweyneynaa inaad booqato warshadeena wakhti kasta oo aad rabto.Waxaan jeclaan lahaa hadda inaan wada yeelano ganacsi wacan oo dheer !!!

Sharaxaada

Xirmadani waxay adeegsataa nidaam u gaar ah oo lysis ah oo si dhakhso ah uga sii deyn kara RNA muunadaha unugyada dhaqameed ee falcelinta RT-qPCR, taas oo meesha ka saaraysa habka nadiifinta RNA waqti-qaadka iyo shaqada badan.Qaabka RNA waxa lagu heli karaa 7 daqiiqo oo kaliya.5× Toos ah RT Mix iyo 2× Toos ah qPCR Mix-SYBR reagents ay bixiso qalabku waxay si degdeg ah oo wax ku ool ah u heli karaan natiijooyinka PCR ee tirada dhabta ah.

5× Toos ah RT Mix iyo 2×QPCR Mix-SYBR toos ah waxay leeyihiin dulqaad adag oo ka hortag ah, iyo lysate ee shaybaarada waxaa loo isticmaali karaa qaab-dhismeedka RT-qPCR si toos ah.Qalabkani waxa uu ka kooban yahay RNA-ga gaarka ah ee xidhiidhka sare ee Foregene reverse transcriptase, iyo Hot D-Taq DNA polymerase, dNTPs, MgCl₂, celiyaha falcelinta, PCR optimizer iyo stabilizer.

Tilmaamaha

200×20μl Rxns, 1000×20μl Rxns

Qaybaha kitka

Tilmaamaha & faa'iidooyinka

■ Fudud oo wax ku ool ah: tignoolajiyada Cell Direct RT, muunadaha RNA waxaa lagu heli karaa 7 daqiiqo oo keliya.

■ Baahida muunada waa yar tahay, maadaama ay yar tahay 10 unug ayaa la tijaabin karaa.

■ Soo saarista sare: waxay si dhakhso ah u ogaan kartaa RNA ee unugyada lagu beeray 384, 96, 24, 12, 6-cells.

■ Tirtiraha DNA-da si dhakhso ah ayuu u saari karaa genome-yada la sii daayay, si weyn ayuu u yareynayaa saameynta natiijooyinka tijaabada dambe.

■ Nidaamka RT iyo qPCR ee la hagaajiyay ayaa ka dhigaya laba-tallaabo dib-u-celinta RT-PCR mid hufan iyo PCR mid gaar ah, oo aad u adkeysi badan ka-hortagga falcelinta RT-qPCR.

Codsiga qalabka

Baaxadda codsiga: unugyada dhaqanka.

- RNA lagu sii daayay muunad lysis: kaliya lagu dabaqi karo qaabka RT-qPCR ee xirmadan.

-Qalabka waxa loo isticmaali karaa ujeedooyinka soo socda: falanqaynta muujinta hidde-sidaha, xaqiijinta saamaynta aamusinta hidde-sidaha ee siRNA, baadhista daroogada, iwm.

Jaantuska

Kaydinta iyo nolosha shelf

Qaybta I ee xirmadan waa in lagu kaydiyaa 4℃;Qaybta II waa in lagu kaydiyaa at -20 ℃.

Foregene Protease Plus II waa in lagu kaydiyaa 4 ℃, ha ku barafaysan -20 ℃.

Reagent 2× Toos ah qPCR Mix-SYBR waa in lagu kaydiyaa -20℃ gudcurka;Haddii si joogta ah loo isticmaalo, waxa kale oo lagu kaydin karaa 4 ℃ kaydinta wakhtiga gaaban (isticmaal 10 maalmood gudahood)Wining the majority in the crucial certifications of its market for Big discounting China High Sensitivity One-Step Probe Rt-Qpcr Kit V2, Tayada waa warshad 'nolosha , Diirada baahida macaamiisha waa isha badbaadada iyo horumarka shirkadda
qiimo dhimis weynShiinaha Taq DNA Polymerase, Qpcr, Shirkaddayadu waxay ballanqaadaysaa: qiimo macquul ah, waqti wax soo saar gaaban iyo adeegga iibka ka dib, waxaan sidoo kale kugu soo dhaweyneynaa inaad booqato warshadeena wakhti kasta oo aad rabto.Waxaan jeclaan lahaa hadda inaan wada yeelano ganacsi wacan oo dheer !!!

QuickEasyTM Cell Direct RT-qPCR Kit -Taqman

Cat.No.DRT-01021/01022

Unug toos ah RT-qPCR oo isticmaalaya ≤ 1000,000 unug

hordhaca alaabta

Alaabtani waxay isticmaashaa nidaam lysis u gaar ah si ay si dhakhso ah RNA uga sii deyso shaybaarada unugyada dhaqameed ee falcelinta RT-qPCR, baabi'inta habka nadiifinta RNA waqti-qaadka iyo shaqada badan, iyo kaliya 7 daqiiqo si loo helo qaabka RNA ee loo baahan yahay, oo leh 5 × Direct RT Mix, 2 × Direct qPCR Mix-Taqman oo ay bixiso xirmadu waxay si dhakhso ah oo hufan u heli kartaa PCR-waqtiga dhabta ah.

5× Direct RT Mix iyo 2× Direct qPCR Mix-Taqman waxay leeyihiin dulqaad adag oo ka hortag ah waxayna samayn karaan dib u habeyn hufan iyo xoojin gaar ah iyadoo la isticmaalayo lysate ee muunada si loo cabbiro qaab template ah.Reagent-ku waxa uu ka kooban yahay Foregene Reverse Transcriptase, Hot D-Taq DNA Polymerase, dNTPs, MgCl₂, Reaction Buffer, PCR Optimizer iyo Stabilizer, kaas oo loo isticmaali karo lysis buffer si dhakhso ah oo sahlan loo ogaado shaybaarada, oo leh sifooyinka dareenka sare, gaar ahaaneed iyo xasiloonida.

Tilmaamaha alaabta

Fudud, tignoolajiyada Cell Direct RT wax ku ool ah oo qaadata ugu yaraan 7 daqiiqo si loo helo muunado RNA.

Shuruudaha muunaduhu waa yar yihiin, iyo ugu yaraan 10 unug oo dhaqan ah ayaa loo isticmaali karaa tijaabada.

Wax soo saarka sare ee helitaanka degdega ah ee RNA ee unugyada dhaqamada leh sida 384, 96, 24, 12, iyo 6-cells plates.

Tirtiraha DNA wuxuu awood u leeyahay inuu si dhakhso ah u saaro genome-yada la sii daayay, isagoo si weyn u yareynaya saameynta natiijooyinka tijaabada dambe.

Nidaamyada RT iyo qPCR ee la hagaajiyay waxay awood u siinayaan laba-tallaabo RT-PCR oo leh qoraal-celin hufan oo waxtar badan, gaar ahaan, iyo dulqaadka falcelinta RT-qPCR oo xooggan.

Codsiga qalabka

Baaxadda codsiga: Unugyada dhaqanka.

Tusaalaha lysis ee loo turjumay RNA: loo adeegsaday kaliya qaab laba-tallaabo RT-qPCR ah.

Xirmooyinka waxaa loo isticmaali karaa ujeedooyinka soo socda: falanqaynta nidaaminta hidde-sidaha, baaritaanka allele, baaritaanka daroogada, iwm.

Xaddidaadaha Kit

jajabyo la xoojiyay ≤ 300 bp.

Xirmooyinka waxaa loo isticmaalaa unugyo dhaqan oo cusub.

Xakamaynta tayada alaabta

Marka loo eego Nidaamka Maareynta Tayada Wadarta ee FOREGENE, qayb kasta oo ka mid ah xirmooyinka taxanaha ah ee tooska ah ee RT-qPCR si adag ayaa loo tijaabiyey dhowr jeer si loo hubiyo isku halaynta iyo xasilloonida tayada xirmooyin kasta.

Qalabka ku jira

*:Cell Lysis, 5× Toos ah RT Mix, 2× Direct qPCR Mix-Taqman si gaar ah ayaa loo iibsan karaa, faahfaahinta waxaa lagu bixiyaa lifaaqa 1 (BOG 13).

Xaaladaha kaydinta

1. Xaaladaha Maraakiibta

Geedi socodka oo dhan ee baakad baraf heerkul hoose ah, si loo hubiyo in qalabku ku jiro gobolka <4 °C.

2. Xaaladaha kaydinta

Ku kaydi qaybta I 4°C iyo qaybta II at -20°C.

Foregene Protease Plus II waa in lagu kaydiyaa 4°C, aan lagu barafaysan -20°C.

Reagent 2× Direct qPCR Mix-Taqman waxa lagu kaydiyaa -20°C, ama 4°C si loogu isticmaalo wakhti gaaban hadii si joogto ah loo isticmaalo (10 maalmood gudahood).

macluumaadka qaybta Kit

Buffer CL: Waxay bixisaa deegaanka looga baahan yahay falcelinta lysis unugga.

Buffer ST: Waxay joojisaa walaxda firfircoon ee lysate si ay uga fogaato saamaynta RT soo socota.

Tirtiraha DNA: Ka saarida DNA-ga, saamaynta ka saarista genome-ka ee tijaabooyinka xiga.

5× Toos ah RT Isku darka: Waxa ku jira xidhiidhka sare ee RNA Foregene Reverse Transcriptase, RNase Inhibitor, dNTPs, stabilizers, enhancers, optimizers, iyo qoraalada dib u celinta ee toosinta ugu fican (Random Primer, Oligo(dT)₁₈Hordhac).

Foregene Protease Plus II: Marka la eego macnaha kaydinta lysis, unugyadu waa lysed si ay u sii daayaan asiidhyada nucleic.

2× Toos ah qPCR Mix-Taqman: reagent ka kooban Hot D-Taq DNA Polymerase, dNTPs, MgCl₂, celiyaha falcelinta, PCR optimizer, iyo xasiliye.

20× ROX Tixraaca Rinjiga: Guud ahaan waxaa loo istcimaalaa Real Time qalabaynta PCR ee ABI, Stratagene iyo shirkado kale, waxaa loo isticmaalaa in lagu hagaajiyo farqiga u dhexeeya tuubooyinka PCR iyo tuubooyinka ay sababto khaladaadka qiyaasta PCR.20× ROX Tixraaca dheeha ee looga baahan yahay qalabyada kala duwan waa ka duwan yahay, isticmaaluhuna wuxuu ku dari karaa iyadoo loo eegayo xoogga lagu taliyey ee qalabka.

RNase-free ddH₂O: Biyo saafi ah oo nadiif ah oo la nadiifiyey oo aan RNase lahayn oo loogu talagalay falcelinta RT-qPCR laba-tallaabo ah.

Ka taxadaritaan:(Hubi inaad si taxadar leh u akhrido taxaddarrada ka hor inta aanad isticmaalin qalabka)

U fiirso habka qalliinka ee tijaabada si aad uga fogaato wasakhowga u dhexeeya shaybaarada.

U fiirso nadaafadda deegaanka tijaabada ah iyo maacuunta si aad uga fogaato faddaraynta RNase iyo xaalufka RNA.

Qaado muunado unug cusub ama si wanaagsan loo xafiday oo waligaa ha isticmaalin muunado unug ah oo dhalaalay oo soo noqnoqda.

5× Toos ah qPCR Mix,2× Toos ah qPCR Mix-Taqman waa in ay ka fogaadaan qaboojin-dhalaalaya soo noqnoqda, haddii kale waxa ay saamayn doontaa qoraalka gadanin iyo waxtarka PCR.

Diyaarintaraashinkaka horhawlgal

Hubi inaad si taxadar leh u akhrido tilmaamaha ka hor intaadan isticmaalin qalabkan.Qalabka Cell Direct RT-qPCR waa mid fudud, ku habboon, oo degdeg ah in lagu shaqeeyo, tilmaamuhuna waxay bixiyaan macluumaad dhammaystiran oo ku saabsan xirmada oo dhan iyo sida saxda ah ee loo isticmaalo.Fadlan diyaari agabka tijaabada ah ee lagama maarmaanka u ah isticmaalka ka hor.

Qalabka iyo qalabka tijaabada

◆ Unugyada dhaqanka.

◆ 1.5 ml ama 2 ml, RNase-/DNase-tuubada centrifuge-ka bilaashka ah, caarada RNase-/DNase-free, 0.2 ml tuubo qPCR oo nadiif ah.

◆ Mashiinka qPCR, pipette, centrifuge miiska sare (≥13,400×g) (waxay kuxirantahay baahida tijaabada ah), iwm.

Badbaadada

◆ Alaabtan waxaa loogu talagalay ujeeddooyin cilmi-baaris cilmiyeed oo keliya, fadlan ha u isticmaalin dawooyinka, bukaan-socodka, cuntada iyo ujeedooyinka qurxinta.

◆ Markaad isticmaalayso kiimikooyin, xidho dharka shaybaadhka, galoofyada, muraayadaha ilaalinta, iwm.

Hawlgalkahagayaal

Nidaamyada Cell Lysis, nidaamyada RT, iyo xirmooyinka kabka falcelinta qPCR ayaa si gooni ah loo iibsan karaa, faahfaahinta ku jirta lifaaqa 1 (BOGGA 13).

Hagaha hawlgalka

A: Tusaalaha sii deynta RNA

1.Cells ayaa horay loo daweeyay: Ku dhaq saxanka dhaqanka unugga qabow PBS, ka dibna lisay unugyada (10-10⁶), 10⁶ Marka loo eego tirada unugyada, waxaa lagula talinayaa Foregene Unugga Qalabka Go'doominta RNA Wadarta (DE-03111) ama Qalabka Go'doominta Xayawaanka ee RNA (DE-03011) ee soo saarista iyo sifaynta RNA.

1.1.Unugyada ku dheggan (24- saxan ceel ah tusaale ahaan)

1.1.1.Go'aami tirada unugyada ceel kasta, go'aami in tirada unugyadu ay tahay 1 × 10⁵, oo isticmaal pipette si aad uga saarto dhexdhexaadiyaha dhaqanka saxanka dhaqanka.

1.1.2.Ku dar 200 μl oo hore loo qaboojiyey 1 × PBS ceel kasta.Ha ku celcelin tuubooyinka oo ha ka saarin PBS ceelasha.Jeedi saxanka oo ka saar inta ugu badan ee PBS ee suurtogalka ah.U gudub talaabada 2.

1.1.3.Saxan dhaqameedka unugga ee kala duwan ama miiska nambarka tixraaca 1-1 ee saxanka dhaqanka unugga ayaa lagu daray precooled 1 × PBS oo loogu talagalay dhaqidda unugyada.

Shaxda1-1: Qiyaasta PBS ee tirada unugyada kala duwan

Ogow：Si loo xaqiijiyo unugyo si adag u dheggan,tiro badan oo ka mid ah luminta unugga ayaa laga fogaadaa marka la dhaqayo.

1.2.Unugyada laalista ama unugyo ku dheggan oo lagu beeray saxanno aan dalool lahayn

1.2.1.Unugyada ku dheggan ee lagu dhaqo saxannada ceelka ee aan badnayn (unugyada laalidu waxay ka bilaabmayaan tallaabada xigta 1.2.2), ururiyaan oo kala saaraan unugyada si waafaqsan habka caadiga ah ee ururinta unugyada, oo ku rid saxan dhaqameed ama tuubo centrifuge;Haddii trypsinization la isticmaalo, waxay u baahan tahay centrifugation si ay u ururiso unugyada iyo in meesha laga saaro trypsin hadhaaga ah, PBS ayaa ku daray unugyo dib loo celiyay unugyo gaar ah si ay u kala firdhiyaan unugyada.

1.2.2.Ka dib markii tirada unugyada la tiriyey, unugyo la jarjaray 1×10⁵ hal ilaa tuubooyinka centrifuge, ku soo ururi unugyo by centrifugation at 1000 × g 10 min.

1.2.3.Ku dar 200 μl PBS tuubada centrifuge, ha ku celcelin tuubooyinka, oo si toos ah u hami PBS.U gudub tillaabada 2. (Haddii ay adagtahay in la soo da'o oo unugyadu dib loo hakiyo, waxa la samayn karaa 1000×g centrifuged 10 min ka dib marka la tuuro sarreenka, pellet-ka unuggu waxa uu sii socdaa talaabada 2)

2.Cell lysis: Ka saar Buffer CL, heerkulkeeda u dhigma heerkulka qolka, DNA Eraser iyo Foregene Protease Plus II, sida ku cad shaxda soo socota 1-2 nidaamka lysis diyaariyey: (Xalka Lysis waa diyaar in la isticmaalo).

Shaxda1-2: jeexid diyaarinta nidaamka (Fiiro gaar ah: diyaarinta barafka)

3. (24 - saxan si fiican tusaale ahaan) Pipette 200 μl of cell lysis sayid sayid isku daraan ceel kasta, si joogto ah u afuufo 5-10 jeer, Incubate at heerkulka qolka (20-25 ℃ ) 5 min.

Ogow：Si looga fogaado samaynta goobooyin, fadlan marka cabirka pipette-ga lagu hagaajiyay 200μl ama ka yar.Unugyadu waxay u muuqan karaan daruuro ka dib lysis, taas oo ah caadi.

4. (24 - saxan ceel tusaale ahaan) waxaa lagu daraa dareeraha 20 μl Buffer ST (nidaamyada kala duwan ee lysis Buffer ST oo lagu daray qadarka lagu muujiyay shaxda 1-3), tuubooyinka soo noqnoqda 5-10 jeer, heerkulka qolka (20-25 ℃) ayaa lagu dhajiyay 2 min.

Ogow：Caarada pipette ayaa lagu tuuray meel ka hooseysa dusha sare, iyadoo la hubinayo in lysate lagu daray,si looga fogaado samaynta goobooyin, fadlan marka cabirka pipette-ga lagu hagaajiyay 200μl ama ka yar.

Shaxda 1-3：Kudar Buffer ST

5. lysate waxaa loo isticmaalaa tijaabooyinka RT-qPCR ee xiga.Haddii tijaabooyinka xiga aan la samayn karin wakhtiga, fadlan ku hay baraf wax ka badan 2hr, oo ku kaydi -20 ℃ ama -80 ℃ (aan ka badnayn saddex bilood).

B: diyaarinta nidaamka RT

1. Ka soo saar 5 × Direct RT Isku qas oo ku rid baraf baraf ah, u daa inuu si dabiici ah u dhalaaliyo, oo si tartiib ah ugu qas isticmaalka dambe;soo saar RNase-free ddH2O oo dhalaali oo dhig baraf baraf si aad hadhow u isticmaasho.U diyaari habka falcelinta barafka sida ku cad Shaxda 2-1 ee hoose.

Shaxda 2-1: Diyaarinta nidaamka falcelinta RT

2.Ka dib markii la dhamaystiro habaynta nidaamka, si tartiib ah isku qasan oo centrifuged kooban shaxda soo socota 2 -2 xaaladaha falcelinta RT.

Shaxda 2-2: RT Reaction dejinta xaaladda

3.Ka dib markii la dhammeeyo falcelinta, badeecada falcelinta ayaa si toos ah loogu dhejiyay barafka loogu talagalay qPCR, fadlan ku rid ilaalinta muddada dheer -20 ℃ ama -80 ℃.

Fiiro gaar ah: Isticmaalka qaab-dhismeedka aan la nadiifin awgeed, da'ayado cad ayaa laga yaabaa inay ka soo baxaan badeecada qoraalka kale.Tani waa dhacdo caadi ah.Centrifuge supernatant isla markiiba tijaabooyinka xiga.

Xalka falcelinta RT ee soo baxday ayaa lagu daraa talaabada xigta ee nidaamka falcelinta PCR-ga ee wakhtiga dhabta ah, waxa lagu talinayaa in lagu daro xaddi u dhexeeya 10-30% ee habka falcelinta.

C: diyaarinta habka falcelinta qPCR

1.Qaddarka ku habboon ee B ee lagu diyaariyey qaabka cDNA tallaabo waafaqsan shaxda soo socota ee 3-1 si loo diyaariyo habka falcelinta.

Fiiro gaar ah: Qadarka template cDNA wuxuu xisaabiyaa 10-30% nidaamka qPCR.Tusaale ahaan, nidaamka 20μl qPCR, ku dar 2-6 μl oo ah lysis buffer, laakiin aan ka badnayn 6 μl.

2.Wanaajinta xaaladaha wanaagsan ee qPCR (heerkulka Annealing, iwm) ee falcelinta qPCR (Xaaladaha falcelinta ee lagu bixiyay Shaxda 3-2).

Xusuusin: Isku day inaad u isticmaasho xaaladaha la hagaajiyay ee falcelinta qPCR si aad u hesho natiijooyin wanaagsan.

Shaxda 3-1: Diyaarinta nidaamka falcelinta PCR

1*: Diiradda koowaad waxaa lagu hagaajin karaa inta u dhaxaysa 50-900 nM marka waxqabadka falcelinta asaasiga ahi liidato.

Fiiro gaar ah: Nidaamka qPCR waxaa lagu hagaajin karaa iyadoo loo eegayo baahida tijaabada ah iyo moodeelka wareegtada fluorescence.Loogu talagalay qPCR gudaha 50μl nidaamka, u hagaaji qiyaasta reagent si siman iyadoo loo eegayo 20μl nidaamka.

2*: Dooro fiirsashada kama dambaysta ah ee ROX Reference Dye iyadoo loo eegayo wareegga kulaylka ee fluorescence.Heerarka dheeha tixraaca ROX ee ugu habboon ee wareegga tireedyada fluorescence ee caadiga ah ayaa lagu muujiyey shaxda hoose:

Shaxda 3-2: xaaladaha falcelinta qPCR ayaa la bixiyay

Fiiro gaar ah: Si loo helo saamaynta qPCR ugu fiican, PCR gradient waxaa loo isticmaali karaa in lagu wanaajiyo xaaladaha falcelinta ee qaabab kala duwan iyo aasaasayaal kala duwan.Xaaladaha falcelinta PCR way kala duwan yihiin iyadoo ku xiran falanqeeyaha fluorescence, template, primer, iwm. Hawlgalka gaarka ah, xaaladaha falcelinta ugu fiican waxay u baahan yihiin in loo qaabeeyo iyadoo loo eegayo shuruudaha gaarka ah ee wareegga kulaylka ee fluorescence, nooca template, cabbirka jajabka xiisaha, isku xigxiga salka ee jajabka la xoojiyay iyo nuxurka GC iyo dhererka heerkulka, oo ay ku jiraan heerkulka waqtiga.

Mabaadi'da naqshadaynta aasaasiga ah ee waqtiga dhabta ah PCR

Horudhac Horudhac iyo Horudhac Hordhac ah

PCR-da-waqtiga-dhabta ah, naqshadaynta asaasiga ahi aad bay muhiim u tahay.Furayaasha waxay la xiriiraan gaarnimada iyo hufnaanta kordhinta PCR, waxaana loo qaabayn karaa iyadoo la tixraacayo mabaadi'da soo socota:

◆ Dhererka hore: 18-30bp.

◆ Waxyaabaha ku jira GC: 40-60%.

◆ Qiimaha Tm: Sooftiweerka naqshadaynta aasaasiga ah, sida Primer 5, ayaa ku siin kara qiimaha Tm ee furaha.Qiimayaasha Tm ee hore iyo kuwa hoose waa inay ahaadaan kuwa ugu dhow ee suurtogalka ah.Qaaciddada xisaabinta Tm waxa kale oo la isticmaali karaa: Tm = 4 °C (G + C) + 2 °C (A + T).Marka la samaynayo PCR, heerkulka ka hooseeya qiimaha Tm ee aasaasiga ah ee 5 °C ayaa guud ahaan loo doortaa heer kulka soo jiidashada (kordhinta u dhiganta heerkulka xididada ayaa kordhin kara qeexida falcelinta PCR).

◆ Qalabka aasaasiga ah iyo PCR:

◆ Naqshadeynta asaasiga ah ee PCR dhererka alaabada kor u qaadida ayaa la door bidaa 100-150bp.

◆ Naqshadaynta aasaasaha aagga qaab-dhismeedka sare ee qaab-dhismeedka waa in laga fogaadaa sida ugu macquulsan.

◆ Ka fogow samaynta 2 ama in ka badan oo saldhigyo is dhammaystiraya oo u dhexeeya 3′ daraf ee aasaasayaasha sare iyo kuwa hoose.

◆ Primer 3′ terminal base kuma jiri karo 3 G ama C oo dheeri ah oo xiriir ah.

◆ Aaladaha laftoodu ma yeelan karaan dhismayaal is kaaba ah, haddii kale qaab-dhismeedka timaha ayaa la samayn doonaa, taasoo saamaynaysa kor-u-qaadista PCR.

◆ ATCG waa in loo qaybiyaa sida ugu siman ee suurtogalka ah ee isku xigxiga asaasiga ah, 3′ terminal base waa in laga fogaadaa sida T.

Lifaaqa1：Ciyo DirectRT-qPCR Kit componenbaakad dheeri ah

1.Cell Lysis Solution