Qalabka yar ee DNA-ga
Sharaxaada Qalabka:
Si dhakhso leh uga nadiifi DNA-ga genomic ee tayada sare leh dhiiga xinjirowga lidka ku ah (<1ml).
◮Wax wasakhaysan RNase ma jiro:Tiirka-DNA-Kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo ka hortagaysa shaybaarka in uu wasakheeyo RNase dibadda ah.
◮Xawaaraha dheereeya:Foregene Protease wuxuu leeyahay dhaqdhaqaaq ka sarreeya borotiinka la midka ah, wuxuuna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 20-80 daqiiqo gudahood.
◮Ku habboon:Centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, mana jirto baahi heerkul hoose oo 4°C ah ama roobka ethanol ee DNA.
◮Badbaadada:Looma baahna soo saarista reagent organic.
◮Tayada sareDNA-da genomic ee la soo saaray ayaa leh jajabyo waaweyn, ma jiro RNA, ma jiro RNase, iyo maadada ion oo aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
◮Nidaamka Micro-elution:Waxay kordhin kartaa diiradda DNA-da genomic, taas oo ku habboon ogaanshaha hoose ama tijaabinta.
Sharaxaada Kit
Qalabkani waxa uu qaadanayaa Foregene Protease Plus cusub iyo nidaamyada BL1 iyo BL2 gaar ah, kuwaas oo si buuxda u dheefshiidi kara shaybaarada dhiigga ka-hortagga xinjirowga lidka ku ah muddo gaaban gudaheed, si looga fogaado hoos u dhaca DNA ilaa heerka ugu sarreeya iyo helitaanka qaddarka ugu badan ee DNA-da genomic.Habka hab-socodka dhiigga ee degdegga ah iyo hawlgalka tiirarka fudud ee DNA-Kaliya ayaa si weyn u fududeynaya soo saarista genome-ka dhiigga, samaynta DNA-da waxaa lagu heli karaa tayo sare iyo nadiif sare 40 daqiiqo gudahood.
Xuubabka silica ee DNA-ka kaliya ee loo isticmaalo tiirarka centrifuge ee xirmadan waa nooc cusub oo walxaha u gaar ah shirkadeena, kaas oo si hufan u xidhi kara DNA-da si gaar ah, oo ka saari kara RNA, wasakhda, borotiinka, ions iyo xeryahooda kale ee organic ee unugyada ilaa xadka ugu badan.Qaybaha DNA-da ee la helay waa kuwo waaweyn, nadiif ah oo sarreeya, tayo deggan oo la isku halleyn karo.Awoodda qaadida ugu badan ee tiirka DNA-kaliya waa 80 μg DNA.DNA-da la helay waxaa loo isticmaali karaa tijaabooyinka bayoloji molecular sida dheefshiidka enzimka, PCR, isku-dhafka koonfurta iyo dhismaha maktabadda.
Qalabku wuxuu farsamayn karaa ilaa 1ml oo dhiig ah markiiba si uu u helo cadad ku filan oo ah DNA-da daahirsanaanta sare leh.
Tilmaamaha
| Qalabka yar ee DNA-ga | |||
| Qalabka ku jira | DE-05111 | DE-05112 | DE-05113 |
| 50 T | 100 T | 250 T | |
| Buffer BL1 | 15 ml | 30 ml | 75 ml |
| Buffer BL2 * | 15 ml | 30 ml | 75 ml |
| Buffer DC | 100 ml | 200 ml | 500 ml |
| Buffer PW * | 25 ml | 50 ml | 125 ml |
| Buffer WB1 | 15 ml | 30 ml | 75 ml |
| Buffer EB | 10 ml | 20 ml | 50 ml |
| Foregene Protease Plus | 1.25 ml | 2.5 ml | 6.5 ml |
| Tiirka-Kaliya DNA | 50 | 100 | 250 |
| Buugga | 1 | 1 | 1 |
*: Buffer BL2 iyo Buffer PW waxa ku jira milix dareere ah oo xanaaqa.Fadlan xidho galoofyada oo qaado talaabooyinka ilaalineed ee khuseeya markaad shaqaynayso.
Tilmaamaha & faa'iidooyinka
-Ma jirto faddarayn RNase: Tiirka DNA-kaliya ee ay bixiso qalabku waxa uu suurtogal ka dhigayaa in RNA laga saaro DNA-da genomic iyada oo aan lagu darin RNase inta lagu jiro tijaabada, iyada oo laga fogaanayo shaybaarka in uu wasakheeyo RNase dibadda ah.
Xawaaraha degdega ah: Foregene Protease Plus waxa uu leeyahay hawl ka sarraysa borotiijiyeyaasha la midka ah, waxayna si dhakhso ah u dheefshiidaa shaybaarada unugyada;Qalliinku waa sahlan yahay, waxaana hawlgalka soo saarista DNA-ga genomic lagu dhamayn karaa 40 daqiiqo gudahood.
-Ku habboonaanta: centrifugation-ka waxaa lagu sameeyaa heerkulka qolka, 4°C centrifugation ama roobka ethanol ee DNA looma baahna.
-Badbaadada: Soo saarista reagents organic lama isticmaalo.
-Tayada sare: Qaybaha DNA-da ee la soo saaray waa waaweyn yihiin, ma jiraan RNA, ma jiro RNase, iyo maadada ion aad u hooseeya, taas oo buuxin karta shuruudaha tijaabooyin kala duwan.
Codsiga qalabka
Waxay ku habboon tahay soo saarista iyo nadiifinta DNA-da genomic ee dhiigga cusub ee xinjirowga lidka ku ah ee barafaysan.
Socodka shaqada
Kaydinta iyo nolosha shelf
Qalabkan waxaa lagu kaydin karaa 12 bilood xaaladaha qalalan ee heerkulka qolka (15-25 ° C);Haddii loo baahdo in lagu kaydiyo wakhti dheer, waxa lagu kaydin karaa 2-8 ° C.
Fiiro gaar ah: Haddii lagu kaydiyo heerkul hooseeya, xalku wuxuu u nugul yahay roobab.Kahor intaan la isticmaalin, hubso inaad geliso xalka qalabka heerkulka qolka muddo wakhti ah.Haddii loo baahdo, ku sii kululee biyaha qubeyska 37°C 10 daqiiqo si aad u milanto daadka, oo ku qas kahor isticmaalka.
Xalka Foregene Protease Plus wuxuu leeyahay qaacido gaar ah, kaas oo firfircoon marka lagu kaydiyo heerkulka qolka muddo dheer (3 bilood);Waxay noqon doontaa mid firfircoon oo xasilloon marka lagu kaydiyo 4 ° C, markaa waxaa lagu talinayaa in lagu kaydiyo 4 ° C, xasuuso inaadan ku haynin -20 ° C.
