Liisaska Qiimaha Shiinaha ee jaban ee Shiinaha Nucleic Acid Soo saarista Reagent Magnetic Bead Habka Qalabka Nucleic Acid Soosaarida Qalabka Rna Soosaarayaasha iyo Soo-saareha Nadiifinta DNA |Foregene

Liiska Qiimaha jaban ee Shiinaha Nucleic Acid Soo saarista Reagent Magnetic Bead Habka Xirmooyinka Soo saarista Ashitada Nukliyeerka ah ee Xirmooyinka Shaybaarka Nadiifinta DNA ee Go'doominta

Liiska Qiimaha jaban ee Shiinaha Nucleic Acid Soo saarista Reagent Magnetic Bead Habka Xirmooyinka Soo saarista Ashitada Nukliyeerka ee Rna Qalabka Sifaynta DNA ee Shaybaarka Sawirka

Sharaxaada Qalabka:

Qalabkani wuxuu isticmaalaa Real-time RT PCR technology (rRT-PCR) si loo ogaado tayada SARS-CoV-2 (ORF1ab hiddo iyo N gene) acids nucleic in nasopharyngeal bini'aadamka ama oropharyngeal swab muunado, by multiplex PCR fluorescent probe method. Tani xirmo ka kooban kontoroolka gudaha si loo qiimeeyo shaybaarka baahida shaybaarka ee tayada Nuclearka, sidaas darteed ma dhamaystiri karo baahida saafiga ah ee aashitada. ogaanshaha degdega ah.

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Waxaan ku sii jirnaa mabda'a aasaasiga ah ee "tayada marka hore, adeegyada marka hore, horumar joogto ah iyo hal-abuurnimo si loo buuxiyo macaamiisha" maamulkaaga iyo "cilad eber, cabashooyin eber" oo ah ujeeddada tayada.Si aan u dhamaystirno shirkadeena, waxaanu siinaa alaabta iyadoo la isticmaalayo tayada sare ee wanaagsan qiimaha iibka macquulka ah ee Liistada Qiimaha jaban ee Shiinaha Nucleic Acid Extraction Reagent Magnetic Bead Method Nucleic Acid Extraction Kits Rna Go'doomin DNA Nadiifinta Qalabka Shaybaarka , Waxaan ku xaqiijinay in aan soo bandhigi karno xalalka tayada sare leh ee heerka macquulka ah, si aad u fiican ka dib iibka.Oo waxaan abuuri doonaa awood cajiib ah.
Waxaan ku sii jirnaa mabda'a aasaasiga ah ee "tayada marka hore, adeegyada marka hore, horumar joogto ah iyo hal-abuurnimo si loo buuxiyo macaamiisha" maamulkaaga iyo "cilad eber, cabashooyin eber" oo ah ujeeddada tayada.Si aan u dhamaystirno shirkadeena, waxaanu siinaa alaabta anagoo adeegsanayna tayada sare ee wanaagsan qiimaha iibka ee macquulka ahShiinaha Nucleic Acid Reagent, Qalabka Soo saarista DNA/RnaWaxaan aaminsanahay adeegeena joogtada ah ee ugu fiican inaad naga heli karto waxqabadka ugu fiican iyo alaabada ugu jaban ee aad naga heli karto muddo dheer .Waxaa naga go'an inaan bixinno adeegyo ka wanaagsan oo aan u abuurno qiimo badan dhammaan macaamiisheena.Waxaan rajeyneynaa inaan wada abuuri karno mustaqbal wanaagsan.

Tilmaamaha

48 Rxns, 96 Rxns

Maya	Qayb	Qadarka				Qaybaha ugu muhiimsan
Maya	Qayb	48 Rxns		96 Rxns		Qaybaha ugu muhiimsan
1	Wakiilka sii daaya acid nucleic	1.4ml/tubo	2 tuubbo	5.3 ml/ dhalo	1 dhalo	Surfactant
2	difaaca RNA	27 μL/tubo	1 tube	53 μL/tubo	1 tube	RNase inhibitor
3	Xalka falcelinta SARS-CoV-2	800 μL/tubo	1 tube	1600 μL/tubo	1 tube	Fure, baadhitaan, falcelin celin, dNTP
4	Isku dhafka enzyme SARS-CoV-2	80 μL/tubo	1 tube	160 μL/tubo	1 tube	Bilawga kulul ee Taq enzyme, M-MLV enzyme
5	SARS-CoV-2 xakamaynta togan	100 μL/tubo	1 tube	100 μL/tubo	1 tube	Plasmid dib-u-habayn oo ka kooban jajab bartilmaameed, RNA
6	SARS-CoV-2 xakamaynta taban	1200 μL/tubo	1 tube	1200 μL/tubo	1 tube	TE baffer

Tilmaamaha & faa'iidooyinka

Degdeg ah oo fudud.
Iska ilaali luminta aashitada nucleic.
Xadka ogaanshaha hooseeya iyo dareenka sare.
Qalabka loo baahan yahay oo hooseeya iyo isticmaalka aadka u dabacsan.

Dareenka ogaanshaha wuxuu gaari karaa heerka ugu hooseeya sida 500 koobi/ml).

GCE/ml	Qiimaha Ct
GCE/ml	N hiddo-wadaha	ORF1ab hidde
2.00E+08	16.43	15.16
2.00E+07	19.48	18.58
2.00E+06	22.71	21.86
2.00E+05	25.95	25.33
2.00E+04	29.30	28.49
2.00E+03	32.37	31.37
2.00E+02	35.32	34.47
2.00E+01	38.29	38.27
2.00E+00	N/A	N/A
2.00E-01	N/A	N/A
NC	N/A	N/A

qalooca dhawaaqa toosan

qalooca xoojinta-RT-qPCR

Sida loo isticmaalo

1. Qalab lagu dabaqi karo

xirmada ogaanshaha covid-19 nucleic acid

2. Habka ogaanshaha

xirmada ogaanshaha covid-19 nucleic acid

Samaynta Tusaalaha: Muunada suufka naafeeya ama oropharyngeal (kaliya 1 tillaabo hawlgal) (Sug 10 daqiiqo)

PCR amplificaton (<= 55min)

Socodka shaqada:

xirmada ogaanshaha covid-19 nucleic acid 4

Kaydinta

laga xidhay iftiinka oo lagu kaydiyay -20 ± 5 ℃;

kaydinta
kaydinta2

Nolosha shelf:1 sano

Waxaan ku sii jirnaa mabda'a aasaasiga ah ee "tayada marka hore, adeegyada marka hore, horumar joogto ah iyo hal-abuurnimo si loo buuxiyo macaamiisha" maamulkaaga iyo "cilad eber, cabashooyin eber" oo ah ujeeddada tayada.Si aan u dhamaystirno shirkadeena, waxaanu siinaa alaabta iyadoo la isticmaalayo tayada sare ee wanaagsan qiimaha iibka macquulka ah ee Liistada Qiimaha jaban ee Shiinaha Nucleic Acid Extraction Reagent Magnetic Bead Method Nucleic Acid Extraction Kits Rna Go'doomin DNA Nadiifinta Qalabka Shaybaarka , Waxaan ku xaqiijinay in aan soo bandhigi karno xalalka tayada sare leh ee heerka macquulka ah, si aad u fiican ka dib iibka.Oo waxaan abuuri doonaa awood cajiib ah.
Liis qiimo jaban oo loogu talagalay Shiinaha Nucleic Acid Reagent, Qalabka Soo saarista DNA/Rna, Waxaan aaminsanahay adeegeena joogtada ah ee ugu fiican inaad naga heli karto waxqabadka ugu fiican oo aad naga heli karto qiimaha ugu yar muddo dheer.Waxaa naga go'an inaan bixinno adeegyo ka wanaagsan oo aan u abuurno qiimo badan dhammaan macaamiisheena.Waxaan rajeyneynaa inaan wada abuuri karno mustaqbal wanaagsan.

Hore: Qiimaha ugu jaban ee Shiinaha ee kaydka Vtm Virus Transport Tubbada Tubada Swab leh

Xiga: Waqtiga Gaaban ee Hogaaminta Shiinaha Nanbei Nidaamka PCR-Waqtiga-dhabta ah ee Shaybaarka PCR

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