Ururku wuxuu hayaa fikradda nidaamka "maamulka sayniska, tayada sare iyo mudnaanta waxtarka leh, iibsadaha ugu sarreeya ee soo saaraha Shiinaha ee 2 × Premix Premix ee aan la nadiifin ee muunad Real-Time Quantitative PCR Direct Multiplex Probe Qpcr Mix Plus U , Our Business is dedicated to giving customers with significant and secure top quality products with our agressive price , fadlan samaynta alaab kasta oo tayo sare leh oo qiimo jaban leh.
Ururku wuxuu sii wadaa fikradda habraaca "maamulka sayniska, tayada sare iyo mudnaanta hufnaanta, wax iibsiga ugu sarreeyaShiinaha Taq DNA Polymerase iyo Qpcr, Shirkaddayadu waxay leedahay xoog badan waxayna leedahay nidaam shabakad iib ah oo joogto ah oo qumman.Waxaan rajeyneynaa inaan xiriir ganacsi oo wanaagsan la sameyn karno dhammaan macaamiisha ka yimid gudaha iyo dibadda iyadoo ku saleysan faa'iidooyinka labada dhinac.
Mabaadi'da naqshadaynta aasaasiga ah ee waqtiga dhabta ah PCR

Horudhac Horudhac iyo Horudhac Hordhac ah

PCR-da-waqtiga-dhabta ah, naqshadaynta asaasiga ahi aad bay muhiim u tahay.Furayaasha waxay la xiriiraan gaarnimada iyo hufnaanta kordhinta PCR, waxaana loo qaabayn karaa iyadoo la tixraacayo mabaadi'da soo socota:

• Dhererka hore: 18-30bp.
• Waxa ku jira GC: 40-60%.
• Qiimaha Tm: Software-ka naqshadaynta aasaasiga ah, sida Primer 5, wuxuu ku siin karaa qiimaha Tm ee asaasiga ah.Qiimayaasha Tm ee hore iyo kuwa hoose waa inay ahaadaan kuwa ugu dhow ee suurtogalka ah.Qaaciddada xisaabinta Tm waxa kale oo la isticmaali karaa: Tm = 4 °C (G + C) + 2 °C (A + T).Marka la samaynayo PCR, heerkulka ka hooseeya qiimaha Tm ee aasaasiga ah ee 5 °C ayaa guud ahaan loo doortaa heer kulka soo jiidashada (kordhinta u dhiganta heerkulka xididada ayaa kordhin kara qeexida falcelinta PCR).
• Qalabka aasaasiga ah iyo PCR:

1. Naqshadeynta asaasiga ah ee PCR dhererka alaabada ayaa la door bidaa 100-150bp.
2. Naqshadeynta aasaasiga ah ee aagga qaab dhismeedka sare ee template waa in laga fogaadaa sida ugu macquulsan.
3. Ka fogow samaynta 2 ama in ka badan oo saldhigyo dhammaystiran oo u dhexeeya 3′ daraf ee hore-u-socodyada sare iyo kuwa hoose.
4. Primer 3′ terminal saldhiga kuma jiri karo 3 dheeraad ah oo isku xigta G ama C.
5. Furayaasha laftoodu ma yeelan karaan dhismayaal is kaaba ah, haddii kale qaab-dhismeedka timaha ayaa la samayn doonaa, oo saamaynaya kor u qaadida PCR.
6. ATCG waa in loo qaybiyaa sida ugu siman ee suurtogalka ah ee isku xigxiga asaasiga ah, iyo 3′ saldhigga terminal waa in laga fogaadaa sida T.

Lifaaqa1:Ciyo DirectRT-qPCR Kit componenbaakad dheeri ah

1.Cell Lysis Solution

 Ururku wuxuu hayaa fikradda nidaamka "maamulka sayniska, tayada sare iyo mudnaanta waxtarka leh, iibsadaha ugu sarreeya ee soo saaraha Shiinaha ee 2 × Premix Premix ee aan la nadiifin ee muunad Real-Time Quantitative PCR Direct Multiplex Probe Qpcr Mix Plus U , Our Business is dedicated to giving customers with significant and secure top quality products with our agressive price , fadlan samaynta alaab kasta oo tayo sare leh oo qiimo jaban leh.
Soo saaraha Shiinaha eeShiinaha Taq DNA Polymerase iyo Qpcr, Shirkaddayadu waxay leedahay xoog badan waxayna leedahay nidaam shabakad iib ah oo joogto ah oo qumman.Waxaan rajeyneynaa inaan xiriir ganacsi oo wanaagsan la sameyn karno dhammaan macaamiisha ka yimid gudaha iyo dibadda iyadoo ku saleysan faa'iidooyinka labada dhinac.

Mabaadi'da naqshadaynta aasaasiga ah ee waqtiga dhabta ah PCR

Horudhac Horudhac iyo Horudhac Hordhac ah

PCR-da-waqtiga-dhabta ah, naqshadaynta asaasiga ahi aad bay muhiim u tahay.Furayaasha waxay la xiriiraan gaarnimada iyo hufnaanta kordhinta PCR, waxaana loo qaabayn karaa iyadoo la tixraacayo mabaadi'da soo socota:

• Dhererka hore: 18-30bp.
• Waxa ku jira GC: 40-60%.
• Qiimaha Tm: Software-ka naqshadaynta aasaasiga ah, sida Primer 5, wuxuu ku siin karaa qiimaha Tm ee asaasiga ah.Qiimayaasha Tm ee hore iyo kuwa hoose waa inay ahaadaan kuwa ugu dhow ee suurtogalka ah.Qaaciddada xisaabinta Tm waxa kale oo la isticmaali karaa: Tm = 4 °C (G + C) + 2 °C (A + T).Marka la samaynayo PCR, heerkulka ka hooseeya qiimaha Tm ee aasaasiga ah ee 5 °C ayaa guud ahaan loo doortaa heer kulka soo jiidashada (kordhinta u dhiganta heerkulka xididada ayaa kordhin kara qeexida falcelinta PCR).
• Qalabka aasaasiga ah iyo PCR:

1. Naqshadeynta asaasiga ah ee PCR dhererka alaabada ayaa la door bidaa 100-150bp.
2. Naqshadeynta aasaasiga ah ee aagga qaab dhismeedka sare ee template waa in laga fogaadaa sida ugu macquulsan.
3. Ka fogow samaynta 2 ama in ka badan oo saldhigyo dhammaystiran oo u dhexeeya 3′ daraf ee hore-u-socodyada sare iyo kuwa hoose.
4. Primer 3′ terminal saldhiga kuma jiri karo 3 dheeraad ah oo isku xigta G ama C.
5. Furayaasha laftoodu ma yeelan karaan dhismayaal is kaaba ah, haddii kale qaab-dhismeedka timaha ayaa la samayn doonaa, oo saamaynaya kor u qaadida PCR.
6. ATCG waa in loo qaybiyaa sida ugu siman ee suurtogalka ah ee isku xigxiga asaasiga ah, iyo 3′ saldhigga terminal waa in laga fogaadaa sida T.

Lifaaqa1:Ciyo DirectRT-qPCR Kit componenbaakad dheeri ah

1.Cell Lysis Solution

Buug-tilmaameedka:

 Degdegga fudud ee unugga tooska ah ee RT-qPCR Kit-Taqman