Soo saaraha iyo alaab-qeybiyaha PCR ee Shiinaha tooska ah |Foregene

xirmo PCR toos ah

Sharaxaada Qalabka:

Xirmadani waxay isticmaashaa hab-dhis u gaar ah si ay si dhakhsa ah u sii deyso DNA-da genomic ee zebrafish iyo unugyada kale ee kalluunka biyaha saafiga ah, dabada dabada ama shaybaarka ukunta kalluunka ee falcelinta PCR, marka waxay si gaar ah ugu habboon tahay baaritaanka hidde-sidaha baaxadda leh.Habka looga sii daayo DNA-da genomic ee kaydiyaha lysis waxa lagu dhammeeyaa 10-30 daqiiqo gudahood 65°C.Looma baahna habab kale sida borotiinka iyo ka saarista RNA, DNA-da raadraaca ee la sii daayayna waxaa loo isticmaali karaa qaab-dhismeedka falcelinta PCR.

2×PCR EasyTM Mix waxay leedahay dulqaad adag oo loogu talagalay horjoogayaasha falcelinta PCR, waxayna isticmaali kartaa lysate ee muunada si loo tijaabiyo qaab-dhismeedka si hufan oo gaar ah.Reagent-kani waxa uu ka kooban yahay ForegeneD-Taq DNAPolymerase, dNTPs, MgCl2, falcelinta falcelinta, hagaajinta PCR iyo xasiliye.Loo isticmaalo iyada oo lala kaashanayo kaydiyaha lysis si dhakhso ah oo fudud u ogaan kara shaybaarada, waxayna leedahay sifooyin dareen sare, gaar gaar ah, iyo xasillooni wanaagsan.

D-Taq DNA polymerase waa DNA polymerase si gaar ah u soo saaray Foregene ee falcelinta PCR tooska ah.D-Taq DNA polymerase waxay leedahay dulqaad xoog leh oo loogu talagalay xakameynta falcelinta PCR ee kala duwan, waxayna si wax ku ool ah u kordhin kartaa tirada DNA-da ee hababka falcelinta kakan ee kala duwan, iyo xawaaruhu wuxuu gaari karaa 2Kb/min, kaas oo si gaar ah ugu habboon falcelinta PCR tooska ah.

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Faahfaahinta Alaabta

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Waxaan si joogto ah fulinta our spirit of ”Innovation keenaya horumarinta, Highly-quality making some subsistence, Management advertising and marketing profit, credit score jiidashada iibsada for PCR toos ah kit, We have been on the Lookout ahead to cooperating with all prospects from in the home and foreign .Waxaa intaa dheer, buuxinta macaamiishu waa raadinta weligeed ah.
Waxaan si joogto ah u fulinaa ruuxeena “Innovation keenaya horumar, tayo sare leh samaynta nolol maalmeedka, Xayeysiinta maamulka iyo faa'iidada suuqgeynta, dhibcaha credit soo jiidashada iibsadayaashaQalabka PC-ga tooska ah, Waxa aanu sugaynay in aanu si dhaw idiinkula shaqayno faa'iidooyinkayada iyo horumarka sare.Waxaan dammaanad qaadnay tayada, haddii macaamiishu aysan ku qanacsanayn tayada alaabada, waxaad ku soo laaban kartaa 7 maalmood gudahood goboladooda asalka ah.

Tilmaamaha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Qaybaha kitka

Qaybta I	Buffer FP
	Kahortagga Protease
	6× DNA Loading Buffer
Qaybta II	2× PCR Fudud^TMIsku qas
Tilmaamaha

Tilmaamaha & faa'iidooyinka

■ Looma baahna nadiifin waqti badan iyo qaali ah.

■ Baahida muunada waa mid yar, in yar oo 1mg kalluun caudal fin ah ama 10 ukun oo kalluun ah ayaa la tijaabin karaa.

■ Looma baahna daawayn gaar ah sida shiidi iyo burburin, qaliinkuna waa sahlan yahay.

■ Nidaamka PCR ee la hagaajiyay wuxuu ka dhigayaa PCR inay yeeshaan gaar gaar ah oo adag iyo dulqaad xooggan ee ka hortagga falcelinta PCR.

Xuduudaha Kit

Codsiga: Kalluunka Zebra iyo kalluunka kale ee biyaha macaan.

DNA-da laga sii daayay muunada lysis: kaliya waxaa loo adeegsaday qaab PCR.

Qalabka waxaa loo isticmaali karaa ujeedooyinka soo socda: aqoonsiga transgenosis, genotyping xayawaanka, iwm.

Socodka shaqada

wk-flw-zebra-kalluunka-tooska-PCR

Kaydinta iyo nolosha shelf

Qaybta I ee xirmadan waa in lagu kaydiyaa 2-8℃.

Reagent Buffer FP waxa lagu kaydin karaa 12 bilood xaaladaha qalalan;waxa lagu kaydin karaa 2-8℃ kaydinta dheer

v Reagent Foregene Protease wuxuu leeyahay qaacido gaar ah.Si loo hubiyo dhaqdhaqaaqeeda iyo xasiloonideeda, fadlan ku kaydi at 4℃ ilaa 12 bilood.

vReagent 6×DNA Loading Buffer waxa lagu kaydin karaa 4℃ ama -20℃ wakhti dheer.

Qaybta II ee xirmadan waa in lagu kaydiyaa -20℃.

vReagent 2×PCR EasyTM Mix, haddii si joogto ah loo isticmaalo, waxa kale oo lagu kaydin karaa 4℃ kaydinta muddada gaaban (isticmaal 10 maalmood gudahood).

We si joogto ah fulinta our spirit of ”Innovation bringing development, Highly-quality making some subsistence, Management advertising and marketing profit, credit score jiidashada iibsada for Big Discount China Cdv AG The Canine Distemper Virus Antigen Degdeg ah Kit , We have been on the Lookout ahead to cooperating with all prospects from in the home and foreign.Waxaa intaa dheer, buuxinta macaamiishu waa raadinta weligeed ah.
Kaarka Tijaabada Shiinaha ee Qiima dhimista Weyn, Tijaabada Tijaabada, Waxaan rajeynaynay inaan si dhow ula shaqeyno faa'iidooyinkayada wadaagga ah iyo horumarka sare.Waxaan dammaanad qaadnay tayada, haddii macaamiishu aysan ku qanacsanayn tayada alaabada, waxaad ku soo laaban kartaa 7 maalmood gudahood goboladooda asalka ah.

Hore: Tayada sare/wadarta guud ee xirmada go'doominta RNA

Xiga: Warshada waxay si toos ah u siisaa Shiinaha Taayirada Rakaabka ah ee ugu Wanaagsan PCR Trailer Tire 155r12 185r14c 195r14c 205/55r16 215/45r17

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