Xirmada Tijaabada Ashitada Nucleic-ka ah ee Shiinaha 2019 Qalabka Tijaabada Degdega ah ee Aashitada Nukliyeerka ahForegene

Qalabka Tijaabada Ashitada Nukliyeerka ah ee Shiinaha 2019 Qalabka Tijaabada Degdega ah

Sharaxaada Qalabka:

Qalabkani wuxuu isticmaalaa Real-time RT PCR technology (rRT-PCR) si loo ogaado tayada SARS-CoV-2 (ORF1ab hiddo iyo N gene) acids nucleic in nasopharyngeal bini'aadamka ama oropharyngeal swab muunado, by multiplex PCR fluorescent probe method. Tani xirmo ka kooban kontoroolka gudaha si loo qiimeeyo shaybaarka baahida shaybaarka ee tayada Nuclearka, sidaas darteed ma dhamaystiri karo baahida saafiga ah ee aashitada. ogaanshaha degdega ah.

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Xalalkayaga si weyn ayaa loo aqoonsaday oo dadku ku kalsoon yihiin waxaana laga yaabaa inay buuxiyaan isbeddel joogto ah oo dhaqaale iyo bulsho ah oo loogu talagalay Qalabka Tijaabada Aashitada Nucleic Acid ee Shiinaha 2019 Qalabka Tijaabada Degdegga ah ee Nukliyeerka Acid (PCR Fluorescence probing), Ku soo dhawow inaad nala soo xiriirto haddii aad xiiseyneyso alaabtayada, waxaan ku siin doonaa qiimo iyo qiimo qaali ah.
Xalkeenna waxaa si weyn loo aqoonsaday oo dadku ku kalsoon yihiin waxaana laga yaabaa inay buuxiyaan isbeddel joogto ah oo dhaqaale iyo kuwa bulsho ahQalabka Tijaabada Shiinaha, Xirmooyinka Tijaabada Degdegga ah, Naqshadeynta, farsamaynta, iibsashada, kormeerka, kaydinta, habka isu-ururinta ayaa dhammaantood ku jira geeddi-socod cilmi-baaris ah oo wax ku ool ah, kordhinta heerka isticmaalka iyo isku-kalsoonaanta calaamadeena si qoto dheer, taas oo naga dhigaysa alaab-qeybiye sare oo ka mid ah afarta qaybood ee alaabooyinka waaweyn ee qashinka gudaha iyo helitaanka kalsoonida macaamiisha si fiican.

Tilmaamaha

48 Rxns, 96 Rxns

Maya	Qayb	Qadarka				Qaybaha ugu muhiimsan
Maya	Qayb	48 Rxns		96 Rxns		Qaybaha ugu muhiimsan
1	Wakiilka sii daaya acid nucleic	1.4ml/tubo	2 tuubbo	5.3 ml/ dhalo	1 dhalo	Surfactant
2	difaaca RNA	27 μL/tubo	1 tube	53 μL/tubo	1 tube	RNase inhibitor
3	Xalka falcelinta SARS-CoV-2	800 μL/tubo	1 tube	1600 μL/tubo	1 tube	Fure, baadhitaan, falcelin celin, dNTP
4	Isku dhafka enzyme SARS-CoV-2	80 μL/tubo	1 tube	160 μL/tubo	1 tube	Bilawga kulul ee Taq enzyme, M-MLV enzyme
5	SARS-CoV-2 xakamaynta togan	100 μL/tubo	1 tube	100 μL/tubo	1 tube	Plasmid dib-u-habayn oo ka kooban jajab bartilmaameed, RNA
6	SARS-CoV-2 xakamaynta taban	1200 μL/tubo	1 tube	1200 μL/tubo	1 tube	TE baffer

Tilmaamaha & faa'iidooyinka

Degdeg ah oo fudud.
Iska ilaali luminta aashitada nucleic.
Xadka ogaanshaha hooseeya iyo dareenka sare.
Qalabka loo baahan yahay oo hooseeya iyo isticmaalka aadka u dabacsan.

Dareenka ogaanshaha wuxuu gaari karaa heerka ugu hooseeya sida 500 koobi/ml).

GCE/ml	Qiimaha Ct
GCE/ml	N hiddo-wadaha	ORF1ab hidde
2.00E+08	16.43	15.16
2.00E+07	19.48	18.58
2.00E+06	22.71	21.86
2.00E+05	25.95	25.33
2.00E+04	29.30	28.49
2.00E+03	32.37	31.37
2.00E+02	35.32	34.47
2.00E+01	38.29	38.27
2.00E+00	N/A	N/A
2.00E-01	N/A	N/A
NC	N/A	N/A

qalooca dhawaaqa toosan

qalooca xoojinta-RT-qPCR

Sida loo isticmaalo

1. Qalab lagu dabaqi karo

xirmada ogaanshaha covid-19 nucleic acid

2. Habka ogaanshaha

xirmada ogaanshaha covid-19 nucleic acid

Samaynta Tusaalaha: Muunada suufka naafeeya ama oropharyngeal (kaliya 1 tillaabo hawlgal) (Sug 10 daqiiqo)

PCR amplificaton (<= 55min)

Socodka shaqada:

xirmada ogaanshaha covid-19 nucleic acid 4

Kaydinta

laga xidhay iftiinka oo lagu kaydiyay -20 ± 5 ℃;

kaydinta
kaydinta2

Nolosha shelf:1 sano

Xalalkayaga si weyn ayaa loo aqoonsaday oo dadku ku kalsoon yihiin waxaana laga yaabaa inay buuxiyaan isbeddel joogto ah oo dhaqaale iyo bulsho ah oo loogu talagalay Qalabka Tijaabada Aashitada Nucleic Acid ee Shiinaha 2019 Qalabka Tijaabada Degdegga ah ee Nukliyeerka Acid (PCR Fluorescence probing), Ku soo dhawow inaad nala soo xiriirto haddii aad xiiseyneyso alaabtayada, waxaan ku siin doonaa qiimo iyo qiimo qaali ah.
Qalabka Tijaabada ee Factory Customized China, Qalabka Tijaabada Degdega ah, Naqshadeynta, processing, iibsiga, kormeerka, kaydinta, habka isu-ururinta ayaa dhamaantood ku jira geeddi-socod cilmi-baaris ah oo wax ku ool ah, kordhinta heerka isticmaalka iyo isku halaynta calaamadeena si qoto dheer, taas oo naga dhigaysa alaab-qeybiye sare oo ka mid ah afarta qaybood ee alaabooyinka waaweyn ee qashinka gudaha gudaha iyo helitaanka kalsoonida macaamiisha si fiican.

Hore: Waqtiga Gaaban ee Hogaaminta Shiinaha Nanbei Nidaamka PCR-Waqtiga-dhabta ah ee Shaybaarka PCR

Xiga: Qiimo jaban ee Shiinaha Virus Novel Detection Reagent Hal Talaabo Ivd Qalabka Tijaabada Degdega ah

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