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Warshada si toos ah Shiinaha Nucleic Acid Aaladda Hubinta Fayraska Degdegga ah ee PCR

Warshada si toos ah Shiinaha Nucleic Acid Degdeg ah Baaritaanka Fayraska PCR ee Qalabka Hubinta Sawirka Guriga
  • Warshada si toos ah Shiinaha Nucleic Acid Aaladda Hubinta Fayraska Degdegga ah ee PCR
  • Warshada si toos ah Shiinaha Nucleic Acid Aaladda Hubinta Fayraska Degdegga ah ee PCR
  • Warshada si toos ah Shiinaha Nucleic Acid Aaladda Hubinta Fayraska Degdegga ah ee PCR
  • Warshada si toos ah Shiinaha Nucleic Acid Aaladda Hubinta Fayraska Degdegga ah ee PCR

Sharaxaada Qalabka:

Xirmadani waxay isticmaashaa nidaam u gaar ah lysis-ka si ay si dhakhso ah uga sii daayaan DNA-da genomic ee dabada, dhegaha, murqaha iyo muunadaha kale ee unugyada ee falcelinta PCR, sidaas darteed waxay si gaar ah ugu habboon tahay baaritaanka hidde-sidaha ballaaran.

Habka looga sii daayo DNA-da genomic ee kaydiyaha lysis waxa lagu dhammeeyaa 10-30 daqiiqo gudahood 65°C.Looma baahna habab kale sida borotiinka iyo ka saarista RNA, DNA-da raadraaca ee la sii daayayna waxaa loo isticmaali karaa qaab-dhismeedka falcelinta PCR.

2× M1-PCR EasyTM Mix waxay leedahay dulqaad xooggan oo loogu talagalay horjoogayaasha falcelinta PCR, waxayna isticmaali kartaa lysate ee muunada si loo tijaabiyo qaab-dhismeedka si hufan oo gaar ah.Reagent-kani waxa uu ka kooban yahay Foregene D-Taq DNAPolymerase, dNTPs, MgCl2, falcelinta falcelinta, hagaajinta PCR iyo xasiliye.

D-Taq DNA polymerase waa DNA polymerase si gaar ah u soo saaray Foregene ee falcelinta PCR tooska ah.D-Taq DNA polymerase waxay leedahay dulqaad xoog leh oo loogu talagalay xakameynta falcelinta PCR ee kala duwan, waxayna si wax ku ool ah u kordhin kartaa tirada DNA-da ee hababka falcelinta kakan ee kala duwan, iyo xawaaruhu wuxuu gaari karaa 2Kb/min, kaas oo si gaar ah ugu habboon falcelinta PCR tooska ah.


Faahfaahinta Alaabta

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Ku adkaysiga "Tayada Sare, Bixinta Degdegga ah, Qiimaha Gardarada", hadda waxaan aasaasnay ​​iskaashi muddo dheer ah macaamiisha si isku mid ah dibadda iyo gudaha iyo hel faallooyinka macaamiisha cusub iyo kuwii hore ee Factory si toos ah Shiinaha Nucleic Acid Rapid PCR TestHubinta Virus-kaAaladaha Guriga, Ku soo dhawoow wixii su'aal ah shirkadeena.Waanu ku faraxsanaan doonaa inaanu kula samayno is-dhexgal ganacsi oo wacan!
Ku adkaysiga "Tayada Sare, Bixinta Degdega ah, Qiimaha Gardarada ah", hadda waxaan la samaynay wada shaqayn wakhti dheer macaamiisha si isku mid ah dibadda iyo gudaha iyo hel faallooyin cusub oo cusub iyo kuwii hore ee macaamiishaTijaabada PCR ee Shiinaha, Hubinta Virus-ka, "Abuur Qiimaha, U Adeegista Macmiilka!"waa ujeeddada aan daba galno.Waxaan si dhab ah u rajaynaynaa in dhammaan macaamiishu ay nala samayn doonaan iskaashi muddo dheer oo faa'iido leh. Haddii aad rabto inaad hesho faahfaahin dheeraad ah oo ku saabsan shirkadeena, Fadlan nala soo xiriir hadda!

Tilmaamaha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Qaybaha kitka

Qaybta I

Xildhiban Buffer

Foregene Protease Plus

6× DNA Loading Buffer

Qaybta II

2× M1 PCR FududTMIsku qas

Tilmaamaha

Tilmaamaha & faa'iidooyinka

■ Looma baahna nadiifin waqti badan iyo qaali ah.

■ Baahida muunada waa yar tahay, in yar oo ah 5mg mouse tissue ama 2-5mm dabada jiirka ayaa la tijaabin karaa.

■ Looma baahna daawayn gaar ah sida shiididda iyo burburinta, qalliinkuna waa sahlan yahay.

■ Nidaamka PCR ee la hagaajiyay wuxuu ka dhigayaa PCR inay yeeshaan gaar gaar ah oo adag iyo dulqaad adag oo loogu talagalay horjoogayaasha falcelinta PCR.

Xuduudaha Kit

Codsiga: dabada jiirka, dhegaha jiirka, muruqa iyo unugyo kale.

DNA-da laga sii daayay muunada lysis: kaliya waxaa loo adeegsaday qaab PCR.

Qalabka waxaa loo isticmaali karaa ujeedooyinka soo socda: aqoonsiga transgenosis, genotyping xayawaanka, iwm.

Socodka shaqada

wk flw-mouse dabada tooska ah PCR

Kaydinta iyo nolosha shelf

Qaybta I ee xirmadan waa in lagu kaydiyaa 2-8℃.

Reagent Buffer AL waxa lagu kaydin karaa 12 bilood xaaladaha qalalan;waxa lagu kaydin karaa 2-8℃ kaydinta dheer

Reagent Foregene Protease wuxuu leeyahay qaacido gaar ah.Si loo hubiyo dhaqdhaqaaqeeda iyo xasiloonideeda, fadlan ku kaydi at 4℃ ilaa 12 bilood.

Reagent 6×DNA Loading Buffer waxa lagu kaydin karaa 4℃ ama -20℃ wakhti dheer.

Qaybta II ee xirmadan waa in lagu kaydiyaa -20℃.

Reagent 2×M1 PCR EasyTMIsku qas, haddii si joogto ah loo isticmaalo, waxa kale oo lagu kaydin karaa 4℃ kaydinta muddada gaaban (la isticmaalo 10 maalmood gudahood).

Ku adkaysiga "Tayada Sare, Bixinta Degdegga ah, Qiimaha Gardarada", hadda waxaan aasaasnay ​​iskaashi muddo dheer ah macaamiisha si isku mid ah dibadda iyo gudaha iyo hel faallooyinka macaamiisha cusub iyo kuwii hore ee Factory si toos ah Shiinaha Nucleic Acid Rapid PCR TestHubinta Virus-kaAaladaha Guriga, Ku soo dhawoow wixii su'aal ah shirkadeena.Waanu ku faraxsanaan doonaa inaanu kula samayno is-dhexgal ganacsi oo wacan!
Warshada si toos ahTijaabada PCR ee Shiinaha, Hubinta Fayraska, "Abuur qiyamka, u adeegida macmiilka!"waa ujeeddada aan daba galno.Waxaan si dhab ah u rajaynaynaa in dhammaan macaamiishu ay nala samayn doonaan iskaashi muddo dheer oo faa'iido leh. Haddii aad rabto inaad hesho faahfaahin dheeraad ah oo ku saabsan shirkadeena, Fadlan nala soo xiriir hadda!

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