Warshada Shiinaha waxay si toos ah u siisaa Xirmooyinka Virus-ka Shiinaha ee PCR-ga Qalabka Tijaabada Aashitada Nukliyeerka ah ee Soo saaraha iyo Iibiyaha |Foregene

Warshada waxay si toos ah u siisaa Xirmooyinka Virus-ka Shiinaha ee PCR-ka

Warshada ayaa si toos ah u siisa Xirmooyinka Virus-ka Shiinaha ee PCR-ga Qalabka Tijaabada ee Aashitada Nucleic-ka ah ee Fayraska DNA-ga Sawirka

Sharaxaada Qalabka:

Qalabkani wuxuu isticmaalaa Real-time RT PCR technology (rRT-PCR) si loo ogaado tayada SARS-CoV-2 (ORF1ab hiddo iyo N gene) acids nucleic in nasopharyngeal bini'aadamka ama oropharyngeal swab muunado, by multiplex PCR fluorescent probe method. Tani xirmo ka kooban kontoroolka gudaha si loo qiimeeyo shaybaarka baahida shaybaarka ee tayada Nuclearka, sidaas darteed ma dhamaystiri karo baahida saafiga ah ee aashitada. ogaanshaha degdega ah.

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Hadafkayagu waa sida caadiga ah inaan u rogno bixiye hal-abuur leh oo ah aaladaha dhijitaalka ah ee teknoolojiyadda sare iyo isgaarsiinta iyadoo la siinayo naqshad qiimo leh oo lagu daray iyo qaab, wax soo saar heer caalami ah, iyo awoodaha dayactirka ee Warshada si toos ah u siisa Xirmooyinka Virus-ka Shiinaha PCR Qalabka Tijaabada Qalabka Nucleic Acid Virus DNA, Wax kasta oo daruuri ah oo adiga kaa yimaada ayaa laguu magdhabi doonaa tixgelinteena ugu weyn!
Hadafkayagu caadi ahaan waa inaan u rogno bixiye hal-abuur leh oo ah qalabka casriga ah ee dhijitaalka ah iyo isgaadhsiinta anagoo bixinayna naqshad qiimo leh oo lagu daray qaab, wax soo saar heer caalami ah, iyo awoodaha dayactirkaShiinaha Degdeg ah, JumloWaxaan raacnaa mabda'a maamulka ee "Tayadu waa sarreyn, Adeeggu waa kan ugu sarreeya, Sumcada waa marka hore", waxaana si daacad ah u abuuri doonaa oo aan la wadaagi doonnaa guusha dhammaan macaamiisha.Waxaan ku soo dhawaynaynaa inaad nala soo xidhiidho wixii macluumaad dheeraad ah oo aanu rajaynayno inaan kula shaqayno.

Tilmaamaha

48 Rxns, 96 Rxns

Maya	Qayb	Qadarka				Qaybaha ugu muhiimsan
Maya	Qayb	48 Rxns		96 Rxns		Qaybaha ugu muhiimsan
1	Wakiilka sii daaya acid nucleic	1.4ml/tubo	2 tuubbo	5.3 ml/ dhalo	1 dhalo	Surfactant
2	difaaca RNA	27 μL/tubo	1 tube	53 μL/tubo	1 tube	RNase inhibitor
3	Xalka falcelinta SARS-CoV-2	800 μL/tubo	1 tube	1600 μL/tubo	1 tube	Fure, baadhitaan, falcelin celin, dNTP
4	Isku dhafka enzyme SARS-CoV-2	80 μL/tubo	1 tube	160 μL/tubo	1 tube	Bilawga kulul ee Taq enzyme, M-MLV enzyme
5	SARS-CoV-2 xakamaynta togan	100 μL/tubo	1 tube	100 μL/tubo	1 tube	Plasmid dib-u-habayn oo ka kooban jajab bartilmaameed, RNA
6	SARS-CoV-2 xakamaynta taban	1200 μL/tubo	1 tube	1200 μL/tubo	1 tube	TE baffer

Tilmaamaha & faa'iidooyinka

Degdeg ah oo fudud.
Iska ilaali luminta aashitada nucleic.
Xadka ogaanshaha hooseeya iyo dareenka sare.
Qalabka loo baahan yahay oo hooseeya iyo isticmaalka aadka u dabacsan.

Dareenka ogaanshaha wuxuu gaari karaa heerka ugu hooseeya sida 500 koobi/ml).

GCE/ml	Qiimaha Ct
GCE/ml	N hiddo-wadaha	ORF1ab hidde
2.00E+08	16.43	15.16
2.00E+07	19.48	18.58
2.00E+06	22.71	21.86
2.00E+05	25.95	25.33
2.00E+04	29.30	28.49
2.00E+03	32.37	31.37
2.00E+02	35.32	34.47
2.00E+01	38.29	38.27
2.00E+00	N/A	N/A
2.00E-01	N/A	N/A
NC	N/A	N/A

qalooca dhawaaqa toosan

qalooca xoojinta-RT-qPCR

Sida loo isticmaalo

1. Qalab lagu dabaqi karo

xirmada ogaanshaha covid-19 nucleic acid

2. Habka ogaanshaha

xirmada ogaanshaha covid-19 nucleic acid

Samaynta Tusaalaha: Muunada suufka naafeeya ama oropharyngeal (kaliya 1 tillaabo hawlgal) (Sug 10 daqiiqo)

PCR amplificaton (<= 55min)

Socodka shaqada:

xirmada ogaanshaha covid-19 nucleic acid 4

Kaydinta

laga xidhay iftiinka oo lagu kaydiyay -20 ± 5 ℃;

kaydinta
kaydinta2

Nolosha shelf:1 sano

Hadafkayagu waa sida caadiga ah inaan u rogno bixiye hal-abuur leh oo ah qalabka casriga ah ee dhijitaalka ah iyo isgaadhsiinta anagoo siinaya naqshad qiimo leh oo lagu daray qaab, wax soo saar heer caalami ah, iyo awoodaha dayactirka ee Warshada si toos ah u siinaysa Xirmooyinka Virus-ka Shiinaha PCR Qalabka Hubinta Nucleic Acid Xirmooyinka Fayraska DNA / Qalabka Nucleic Acid Extraction Kits PCR Kit, Wax kasta oo daruuri ah oo adiga kaa yimaada ayaa laguu xisaabin doonaa magdhowgayaga!
Warshada si toos ah u keenShiinaha Degdeg ah, JumloWaxaan raacnaa mabda'a maamulka ee "Tayadu waa sarreyn, Adeeggu waa kan ugu sarreeya, Sumcada waa marka hore", waxaana si daacad ah u abuuri doonaa oo aan la wadaagi doonnaa guusha dhammaan macaamiisha.Waxaan ku soo dhawaynaynaa inaad nala soo xidhiidho wixii macluumaad dheeraad ah oo aanu rajaynayno inaan kula shaqayno.

Hore: Qalabka Tijaabada Baadhista Antigen-ka ee China ee Qalabka Tijaabada Degdega ah ee Jumlada

Xiga: Gaadhida Degdega ah ee Shiinaha Co-19 Xirmada ogaanshaha Antigen Antibody oo wata Swab iyo Aaladda Ururinta Candhuufta

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