Ilaha Warshada Shiinaha Nidaamka Soo saarista Ashitada Nukliyeerka ah ee tooska ah ee DNA-da iyo Soo-saareha Nadiifinta Rna iyo Alaabta |Foregene

Ilaha warshada Nidaamka Soo saarista Ashitada Nukliyeerka Toos ah ee DNA iyo Sifaynta Rna

Isha warshadda Nidaamka Soo saarista Ashitada Nukliyeerka ah ee Toos ah ee DNA iyo Nadiifinta Rna Sawirka Sifaysan

Sharaxaada Qalabka:

Si toos ah u isticmaal polysaccharide polyphenol iniin lysate qaab template ah kor u qaadida PCR, iyada oo aan la soo saarin DNA gaar ah, degdeg iyo dareen sare.Nidaamka ka hortagga wasakheynta ee UNG ayaa lagu daray si looga hortago waxyaabo been abuur ah.

- Ma jiro waqti-qaadasho iyo nadiifin DNA qaali ah.

- Baahida muunadku way yar tahay, kaliya qaado hal abuur.

- Looma baahna daawayn gaar ah sida shiidi iyo burburin, qalliinkuna waa sahlan yahay.

-Nidaamka PCR ee la wanaajiyey waxa uu awood u siinayaa PCR in uu yeesho gaar gaar ah iyo dulqaad adag oo loogu talagalay hor joogayaasha falcelinta PCR.

- Nidaamka PCR-ka-hortagga wasakheynta 2× Seed PCR EasyTM Mix (UNG), kaas oo si wax ku ool ah u baabi'in kara wasakhowga ay keenaan alaabta PCR iyo hubinta gaarnimada iyo saxnaanta xoojinta.

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Faahfaahinta Alaabta

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Inaga oo ay taageerayaan koox sare oo IT ah oo takhasus leh, waxaan ku siin karnaa taageero farsamo oo ku saabsan iibka ka hor iyo adeegyada iibka ka dib ee ilaha Warshada Nidaamka Soo saarista Nucleic Acid ee tooska ah ee DNA iyo Nadiifinta Rna, Haddii faahfaahin dheeraad ah loo baahdo, fadlan na soo wac wakhti kasta!
Iyadoo ay taageerayaan koox sare oo IT ah oo takhasus leh, waxaan ku siin karnaa taageero farsamo oo ku saabsan iibka ka hor iyo adeegyada iibka ka dib, Waxaan ku dadaaleynaa heer sare, horumar joogto ah iyo hal-abuurnimo, waxaa naga go'an inay naga dhigaan "kalsoonida macaamiisha" iyo "doorashada koowaad ee alaab-qeybiyeyaasha mashiinka qalabka injineernimada.Na door, idinkoo wadaagaya xaalad guul-guul ah!

Tilmaamaha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Qaybaha kitka

Qaybta I	Buffer P1
	Buffer P2
	6×DNA Loading Buffer
Qaybta II	2× Caleen PCR Fudud^TMIsku qas(UNG)

Tilmaamaha & faa'iidooyinka

■ Ma jiro waqti-qaadasho iyo nadiifin DNA-da qaali ah
■ Waxyaabo yar
■ Fudud-muunada waxaa lagu soo rogi karaa falcelinta PCR ama falcelinta lysis iyada oo aan la jarin ama la shiidi
■ 2 × Isku qas, yaraynta khaladaadka muunada rarida iyo wakhtiga diyaarinta nidaamka falcelinta
■ Diyaarinta qaab-dhaqso leh ayaa lagu dhammeyn karaa 10 daqiiqo, falcelinta PCR waxay ku dhameyn kartaa ugu yaraan 50 daqiiqo
■ Dareen-celinta-lysis-ka sare ee fal-celinta waxaa lagu dhammayn karaa saxan PCR 96-wanaagsan

Xuduudaha Kit

Codsiga: Aqoonsiga hidde ahaan la beddelay, genotyping, iwm.
Tusaale: caleen geed
Qiyaasta: Dhexroorka 2-3mm (hab toos ah), dhexroor 5-7mm (qaabka lysis)
Qiyaasta ogaanshaha: jajabka bartilmaameedka ≤1kb

Socodka shaqada

Caleenta Dhirta ee tooska ah ee PCR Kit-UNG4

Electroferogram

Caleenta Geedka Tooska ah ee PCR Kit-UNG5

Laba toddobaad oo natiijooyinka ogaanshaha PCR Mix ee hoos yimaada xaaladda wasakhaynta alaabta kor u qaadida PCR. Iyadoo ay taageerayaan koox sare oo IT ah oo takhasus leh, waxaan ku siin karnaa taageero farsamo oo ku saabsan iibka ka hor iyo adeegyada iibka ka dib ee ilaha Warshada ee Nidaamka Soosaarka Nucleic Acid ee DNA iyo Nadiifinta Rna, Haddii faahfaahin dheeraad ah loo baahdo, fadlan na soo wac wakhti kasta!
Ilaha warshadda , Waxaan ku dadaalnaa si heer sare ah, horumar joogto ah iyo hal-abuurnimo, waxaa ka go'an in ay naga dhigaan "kalsoonida macaamiisha" iyo "doorashada kowaad ee brand accessories mishiinka injineernimada".Na door, idinkoo wadaagaya xaalad guul-guul ah!

Hore: Xirmada Soo saarista DNA-da ee Imaatinka Cusub ee Shiinaha

Xiga: Fayraska Fayraska ugu sarreeya DNA-da iyo Xirmada Go'doominta Rna-da ee Soo Saarista Nucleic Acid Reagent ee PCR-da ogaanshaha waqtiga dhabta ah

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