Waxaan xooga saaraynaa kobcinta iyo soo bandhigista xalal cusub suuqa oo kaliya sanad walba il Warshada High Fidelity Hotstart Taq Mix PCR Kit PCR Master Mix 2 × A8 Fasthifi PCR Mastermix , We're devoted to provide skilled purification technology and options for you personally!
Waxaan xoogga saareynaa kobcinta waxaanan soo bandhignaa xalal cusub oo suuqa ku saabsan sanad walbaQalabka PCR ee Shiinaha iyo PCR, Ilaa hadda, liiska alaabta si joogto ah ayaa loo cusboonaysiiyay oo soo jiidatay macaamiisha adduunka oo dhan.Xaqiiqooyinka qoto dheer waxaa badanaa laga helaa mareegahayaga waxaana ku siin doona adeeg lataliye tayo sare leh kooxdayada iibka kadib.Waxay kaa caawin doonaan inaad si qoto dheer u garato alaabtayada oo aad samayso gorgortan ku qanacsan.Shirkadda aado warshadeena Brazil sidoo kale waa la soo dhawaynayaa wakhti kasta.Rajo inaad hesho su'aalahaaga wada shaqayn kasta oo farxad leh.
Buugga tilmaamaha:

Waxaan xooga saaraynaa kobcinta iyo soo bandhigista xalal cusub suuqa oo kaliya sanad walba il Warshada High Fidelity Hotstart Taq Mix PCR Kit PCR Master Mix 2 × A8 Fasthifi PCR Mastermix , We're devoted to provide skilled purification technology and options for you personally!
Isha warshaddaQalabka PCR ee Shiinaha iyo PCR, Ilaa hadda, liiska alaabta si joogto ah ayaa loo cusboonaysiiyay oo soo jiidatay macaamiisha adduunka oo dhan.Xaqiiqooyinka qoto dheer waxaa badanaa laga helaa mareegahayaga waxaana ku siin doona adeeg lataliye tayo sare leh kooxdayada iibka kadib.Waxay kaa caawin doonaan inaad si qoto dheer u garato alaabtayada oo aad samayso gorgortan ku qanacsan.Shirkadda aado warshadeena Brazil sidoo kale waa la soo dhawaynayaa wakhti kasta.Rajo inaad hesho su'aalahaaga wada shaqayn kasta oo farxad leh.

Tilmaamaha Falanqaynta Dhibaatada

The following is an analysis of the problems that may be encountered in the extraction of plant genomic DNA, hoping to be helpful to your experiments. In addition, for other experimental or technical problems other than operation instructions and problem analysis, we have dedicated technical support to help you. If you have any needs, please contact us: 028-83360257 or E-mali: Tech@foregene.com.

Waxsoosaar hooseeya ama DNA-da oo aan lahayn

Badanaa waxaa jira arrimo badan oo saameeya soo-saarka DNA-da, oo ay ku jiraan isha muunadda, da'da muunada, xaaladaha kaydinta muunadda, iyo qalliinka.

DNA-da genomic lama heli karo inta lagu jiro soo saarista

1. Shaybaarada unugyada ayaa si khaldan loo kaydiyaa ama loo kaydiyaa wakhti aad u dheer, taasoo keentay hoos u dhaca DNA-da genomic.

Talo-soo-jeedin: Ku kaydi shaybaarada unugga ee dareeraha nitrogen ama -20°C;isku day inaad u isticmaasho muunado dhowaan la ururiyey si loo soo saaro DNA-da genomic.

2. Qadar muunad aad u yar ayaa laga yaabaa in ay keento in DNA-da genomiga ah ee u dhigma aan la soo saarin.

Talo-soo-jeedin: Shaybaarrada unugyada ee kaydsan muddo dheer ama leh burbur DNA-ga genomic oo daran, qaddarka shaybaarrada unugyada si habboon ayaa loo kordhin karaa si loo soo saaro DNA-ga genomic oo badan.Qadarka muunada waxaa lagu go'aamin karaa iyadoo loo eegayo baahida DNA-da, laakiin muunadda cusub waa inaysan dhaafin 100mg, muunadda qallalanna waa inaysan ka badnaan 30mg.

3. Muunada laguma darin nitrogen dareere ah ama laguma meeleeyo waqti aad u dheer ka dib nitrogen dareeraha ah.

Talo soo jeedin: Inta lagu jiro soo saarista DNA-da, muunadku waxa uu u baahan yahay in si buuxda loo geliyo nitrogen dareere ah si uu u jebiyo gidaarka unugga;Shiidi ka dib, fadlan ku wareeji budada muunada PL1 oo lagu sii kululeeyay 65°C sida ugu dhakhsaha badan (marka budada dhulka la dhalaaliyo, DNA-da genomic waxay bilaabi doontaa inay si degdeg ah u hoos u dhacdo).

4. Kaydinta khaldan ee Foregene Protease waxay keenaysaa hoos u dhac ama dhaqdhaqaaq la'aan.

Talo soo jeedin: Xaqiiji xaaladaha kaydinta Foregene Protease ama ku beddel Foregene Protease cusub oo loogu talagalay hydrolysis enzymatic.

5. Xirmada si aan habboonayn ayaa loo kaydiyaa ama loo kaydiyaa muddo aad u dheer, taasoo keenaysa in qaybo ka mid ah qalabkani ay fashilmaan.

Talo-soo-jeedin: Iibso xirmo soo saarista DNA-ga geedka cusub ee hawlaha la xiriira.

6. Isticmaalka aan habboonayn ee qalabka.

Soo-jeedin: Iibso Qalabka Go'doominta DNA-ga Dhirta oo u heellan shaybaarrada soo saarista iyo nadiifinta DNA-da hidde-sideyaasha.

7. Buffer WB adigoon ku darin anafaqo leh ethanol.

Talo soo jeedin: Hubi inaad ku darto mugga saxda ah ee ethanol buuxa ee Buffer WB.

8. Eluent si sax ah looguma daadin xuubka silica.

Soo jeedin: Ku dar eluent horay loo sii kululeeyay 65℃hoos u dhig ilaa dhexe ee xuubka silica jel, oo ku dhaaf heerkulka qolka 5 daqiiqo si loo kordhiyo waxtarka sare.

Soo saarista si loo helo DNA genomic-soo-saarka hooseeya

1. Tijaabada si aan habboonayn ayaa loo kaydiyaa ama loo kaydiyaa wakhti aad u dheer, taasoo keentay hoos u dhaca DNA-da genomic.

Talo soo jeedin: Ku kaydi shaybaarada unugyada at -20℃;isku day in aad isticmaasho muunado unug dhawaan la ururiyey si loo soo saaro DNA-da genomic.

2. Haddii cadadka shaybaarka unuggu aad u yar yahay, DNA-ga la soo saaray wuu yaraan doonaa.

Talo soo jeedin: Qaar ka mid ah muunadaha dhirta ayaa qani ku ah biyaha, sida dhirta biyaha sida algae, iwm., qiyaasta si habboon ayaa loo kordhin karaa ama biyaha ayaa fuuq-baxa ka hor qaliinka.

3. Tijaabooyinku si fiican looguma dhadhamin nitrogen dareere ah ama waxa lagu daayay heerkulka qolka muddo dheer ka dib marka la shiido.

Soo jeedin: Shiididda nitrogen dareeraha ah waa in ay ahaataa mid ku filan, iyo gidaarka muunada waa in la jebiyaa intii suurtagal ah;Isla markiiba ka dib shiididda, budada saamiga waa in loo wareejiyaa 65℃Preheated Buffer PL1 ee talaabada xigta.

4. Aan la isticmaalin qalabka saxda ah.

Talo-soo-jeedin: Isticmaal qalab go'doominta DNA-ga dhirta si aad u soo saarto oo aad u nadiifiso DNA-da hidde-sideyaasha.

5. Kaydinta khaldan ee Foregene Protease waxay keenaysaa hoos u dhac ama dhaqdhaqaaq la'aan.

Talo soo jeedin: Xaqiiji xaaladaha kaydinta Foregene Protease ama ku beddel Foregene Protease cusub oo loogu talagalay hydrolysis enzymatic.

6. Dhibaato ilbax ah

Talo soo jeedin: Fadlan u isticmaal Buffer EB si aad u qeexdo;haddii aad isticmaalayso ddH₂O ama kuwa kale, hubi in pH-ga eluent-ku uu u dhexeeyo 7.0-8.5.

7. Ilmaha oo aan si sax ah loo dareerin

Talo soo jeedin: Fadlan ku dar dhibicda elutionka badhtamaha xuubka silica oo ku dhaaf heerkulka qolka 5 daqiiqo si loo kordhiyo waxtarka sare.

8. Mugga eluent aad buu u yar yahay

Soo jeedin: Fadlan u isticmaal eluent si aad u hesho DNA genomic sida waafaqsan tilmaamaha, ugu yaraan aan ka yarayn 100μl.

DNA-ga genomic ee la soo saaray oo leh daahir hoose

Nadiifinta hoose ee DNA-da genomic waxay u horseedi doontaa guuldarro ama saameyn liidata ee tijaabooyinka hoose, sida: enzyme lama gooyn karo, iyo jajabka hidda-wadaha bartilmaameedka ah ma heli karo PCR.

1. Wasakhaynta borotiinka kala duwan, wasakhaynta RNA.

Falanqaynta: Buffer PW looma isticmaalin in lagu dhaqo tiirka;Buffer PW looma isticmaalin in lagu dhaqo tiirka xawaaraha dhexe ee saxda ah.

Talo soo jeedin: isku day inaad hubiso inaysan jirin wax roobaad ah oo ka da'aya korka sare marka sareynta la dhex maro tiirka;Hubi inaad ku dhaqdo tiirka nadiifinta Buffer PW si waafaqsan tilmaamaha, tallaabadan lama dhaafi karo.

2. wasakhowga wasakhda wasakhaysan.

Falanqaynta: Tiirka dhaqidda ee Buffer WB waa laga saaray ama hal mar kaliya ayaa la dhaqay, taasoo keentay faddarayn ionic ah.

Talo soo jeedin: Hubi inaad laba jeer ku maydho Buffer WB sida ku cad tilmaamaha si aad meesha uga saarto ion haraaga intii suurtogal ah.

3. Wasakhda RNase.

Falanqaynta: Exogenous RNase waxaa lagu daraa kaydka;Hawlgalka dhaqidda khaldan ee Buffer PW wuxuu dhalin doonaa haraaga RNase wuxuuna saameeyaa hawlaha tijaabada ah ee RNA-da hoose, sida qorista vitro.

Soo jeedin: Xirmooyinka soo saarista aashitada nukliyeerka ee taxanaha ah ayaa ka saari kara RNA iyada oo aan la helin RNase dheeraad ah, iyo dhammaan reagen-yaasha ku jira Qalabka Go'doominta DNA uma baahna RNase;Hubi inaad ku dhaqdo tiirka nadiifinta Buffer PW si waafaqsan tilmaamaha, tallaabadan lama dhaafi karo.

4. Haraaga ethanol.

Falanqaynta: Ka dib markii lagu dhaqay tiirka sifaynta ee Buffer WB, ma jirto xarun dhexe oo tuubo oo madhan ah.

Talo-soo-jeedin: Raac tilmaan-bixinta ku-meel-gaadhka ah ee tuubbada madhan.

Buugga tilmaamaha:

Buugga Tilmaamaha Qalabka Go'doominta Dhirta ee DNA