Xirmada Soosaarida Gel-ga ee DNA-ga
Sharaxaada Qalabka:
Si dhakhso leh oo hufan uga soo kabso jajabyada DNA-da 20bp-10kb ee jelka agarose.
◮Soo kabashada DNA-da ballaaran:Qaybaha DNA-da oo gaaban sida 30bp iyo inta u weyn sida 10kb waa la soo celin karaa.
◮Waxtarka soo kabashada sare:Waxtarka ugu sarreeya ee soo kabashada wuxuu gaari karaa in ka badan 80%.
◮Nadaamka yar-yar:ugu yaraan 30μl xal elution waxaa loo isticmaali karaa elution, kaas oo si wax ku ool ah u kordhin kara fiirsashada ee jajabkii DNA soo kabsaday.
◮Xawaaraha dheereeya:Si fudud in loo shaqeeyo, soo kabashada jajabka DNA-da waxaa lagu dhameyn karaa 15 daqiiqo gudahood.
◮Badbaadada:Looma baahna soo saarista reagent organic.
◮Tayada sareQaybaha DNA-da ee la helay waa nadiif sare, kuwaas oo la kulmi kara tijaabooyin kala duwan oo xiga.
Sharaxaada
Xirmadani waxay isticmaashaa tiirka lafdhabarta iyo caanaha ay samaysay shirkadeena, kaas oo si hufan uga soo kaban kara jajabyada DNA-da ee nadiifka ah ee jelka agarose.Qalabku wuxuu soo ceshan karaa jajabyada DNA-da oo ballaaran.Xaaladaha caadiga ah, jajabyada DNA ee 30bp-10kb waa la soo celin karaa.Ugu yaraan 30 μl oo eluent ah ayaa loo isticmaali karaa si loo kordhiyo uruurinta DNA-da la soo kabsaday.
Qalabka waa sahlan tahay in lagu shaqeeyo wuxuuna leeyahay talaabooyin yar.Waxay u baahan tahay oo kaliya dhowr sentifugation si ay u baaraan shaybaaro badan isku mar, iyo daahir sare oo jajabka DNA soo kabsaday waxaa lagu heli karaa 15 daqiiqo gudahood.
Tilmaamaha
50 diyaarin
Buffer GE: Ku milmi xannibaadda jel 60°C si aad u sii deyso jajabyada DNA-da ee jelka.
Buffer WB1: Ka saar ion milixda hadhaaga ah ee DNA-da.
Buffer EB: ka saar DNA-da xuubka tiirka sifaynta.
Tiirka-DNA-Kaliya: Xayeysiiska gaarka ah ee jajabyada DNA-da ee jelka.
Qaybaha kitka
| Buffer GE* |
| Buffer WB1 |
| Buffer EB |
| Tiirka-Kaliya DNA |
| Tilmaamaha |
Tilmaamaha & faa'iidooyinka
Soo-kabashada DNA-da oo ballaadhan: jajabyada DNA-da oo gaaban sida 30bp iyo inta u badan 10kb waa la soo ceshan karaa.
Waxtarka soo kabashada sare: Waxtarka soo kabashada ugu sareysa waxay gaari kartaa in ka badan 80%.
Nidaamka yaryar ee nidaamka: ugu yaraan 30μl xal elution ayaa loo isticmaali karaa elution, taas oo si wax ku ool ah u kordhin karta xoojinta jajabyada DNA-da ee la soo kabsaday.
Xawaaraha degdega ah: fududahay in la shaqeeyo, soo kabashada jajabka DNA-ga waxaa lagu dhameyn karaa 15 daqiiqo gudahood.
-Badbaadada: Soo saarista reagents organic looma baahna.
-Tayada sare: Qaybaha DNA-da ee la helay waa nadiif sare, kuwaas oo la kulmi kara tijaabooyin kala duwan oo xiga.
Codsiga qalabka
Qalabkani wuxuu ku habboon yahay in lagu soo celiyo jajabkii DNA-da (30bp-10kb) ee jel-ka agarose.
Socodka shaqada
Kaydinta iyo nolosha shelf
Qalabkan waxa lagu kaydin karaa 12 bilood marka ay jiraan xaalado qallalan oo heerkul ah (15-25°C), haddii loo baahdo in lagu kaydiyo wakhti dheer, waxa lagu kaydin karaa 2-8°C.
Fiiro gaar ah: Haddii lagu kaydiyo heerkul hooseeya, xalku wuxuu u nugul yahay roobab.Kahor intaan la isticmaalin, hubso inaad geliso xalka qalabka heerkulka qolka muddo wakhti ah.Haddii loo baahdo, ku sii kululee biyaha qubeyska 37°C 10 daqiiqo si aad u milanto daadka, oo ku qas kahor isticmaalka.
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