Shiinaha Qeexid Sare Oo Soo Saarista Ashitada Nukliyeerka Shiinaha Yc-701 Soo Saare iyo Soo-saariyahaForegene

Qeexida sare ee Shiinaha Nucleic Acid Sifaynta Soo Saaridda Yc-701 Cov Nucleic Acid

Qeexida sare ee Sifaynta Asiidhka Nucleic-ka Shiinaha Yc-701 Cov Nukleic Acid Sawirka

Sharaxaada Qalabka:

Xirmadani waxay isticmaashaa nidaam u gaar ah si ay si dhakhso ah u sii daayaan DNA-ga genomic ee shaybaarada unugyada xoolaha ee falcelinta PCR, sidaa darteed waxay si gaar ah ugu habboon tahay baaritaanka hidde-sideyaasha baaxadda leh.

Habka looga sii daayo DNA-da genomic ee kaydiyaha lysis waxa lagu dhammeeyaa 10-30 daqiiqo gudahood 65°C. Looma baahna habab kale sida borotiinka iyo ka saarista RNA, DNA-da raadraaca la sii daayayna waxa loo isticmaali karaa qaab fal-celin PCR ah.

2×PCR Fudud^TMMix (UNG) waxay isticmaashaa dUTP halkii ay ka isticmaali lahayd dTTP iyadoo ku saleysan 2×PCR Fudud^TMIsku qas, oo ku dara UNG enzyme (Uracil-N-glycosylase) kaas oo hoos u dhigi kara template ka kooban dUTP isku mar.Kahor falcelinta PCR-ga, ensaymka UNG waxaa loo isticmaalaa hoos u dhigista alaabta PCR ee ay ku jirto uracil.Enzyme UNG wax saameyn ah kuma yeelan doono qaab-dhismeedka aan ku jirin uracil, si loo hubiyo gaar ahaanta iyo saxnaanta xoojinta iyo ka hortagga suurtagalnimada wasakheynta alaabta PCR inta lagu jiro baaritaanka hidda-socodka ballaaran.

D-Taq DNA polymerase waa DNA polymerase si gaar ah u soo saaray Foregene ee falcelinta PCR tooska ah.D-Taq DNA polymerase waxay leedahay dulqaad xoog leh oo loogu talagalay xakameynta falcelinta PCR ee kala duwan, waxayna si wax ku ool ah u kordhin kartaa tirada DNA-da ee hababka falcelinta kakan ee kala duwan, iyo xawaaruhu wuxuu gaari karaa 2Kb/min, kaas oo si gaar ah ugu habboon falcelinta PCR tooska ah.

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Faahfaahinta Alaabta

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Waxaan ku dheggannahay mabda'a ah "tayada marka hore, adeegga ugu horreeya, hagaajin joogto ah iyo hal-abuurnimo si aan ula kulanno macaamiisha" maamulka iyo "cilladda eber, cabashooyinka eber" sida ujeedada tayada.Si aan u dhamaystirno adeegeena, waxaanu ku siinaynaa alaabta tayada wanaagsan ee qiimaha macquulka ah ee Qeexida Sare ee Shiinaha Nucleic Acid Sifeynta Soo saarista Yc-701 Cov Nucleic Acid, Xoog saarista khaaska ah ee ku saabsan baakadaha badeecada si looga fogaado waxyeelo kasta inta lagu jiro gaadiidka.
Waxaan ku dheggannahay mabda'a ah "tayada marka hore, adeegga ugu horreeya, hagaajin joogto ah iyo hal-abuurnimo si aan ula kulanno macaamiisha" maamulka iyo "cilladda eber, cabashooyinka eber" sida ujeedada tayada.Si aan u dhamaystirno adeegeena, waxaanu ku siinaa alaabta tayada wanaagsan qiimo macquul ahNidaamka Soo saarista Ashitada Nukliyeerka ee Shiinaha, Qalabka Soosaarka Asiidhka Nucleic, Waxaan si sii kordheysa u ballaarineynaa saamigayaga suuqa caalamiga ah iyadoo ku saleysan alaab tayo leh, adeeg heer sare ah, qiimo macquul ah iyo gaarsiinta waqtiga.Fadlan nala soo xidhiidh wakhti kasta wixii macluumaad dheeraad ah.

Tilmaamaha

50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns

Qaybaha kitka

Qaybta I	Buffer AL
	Kahortagga Protease
	6× DNA Loading Buffer
Qaybta II	2× PCR Fudud^TMIsku qas(UNG)

Tilmaamaha & faa'iidooyinka

■ Ma jiro waqti-qaadasho iyo nadiifin DNA-da qaali ah

■ Baahida muunada waa yar tahay, in yar oo 5mg unug xayawaan ah ayaa la tijaabin karaa.

■ Looma baahna daaweyn gaar ah sida shiidi iyo burburin, qalliinkuna waa sahlan yahay.

■ Nidaamka PCR ee la hagaajiyay wuxuu ka dhigayaa PCR inay yeeshaan gaar gaar ah oo adag iyo dulqaad xooggan ee ka hortagga falcelinta PCR.

■ Nidaamka PCR-ka-hortagga wasakheynta 2×PCR Easy^TMIsku-darka (UNG) waxay si wax ku ool ah u baabi'isaa wasakhowga ay keenaan alaabada PCR waxayna hubisaa gaar ahaan iyo saxnaanta kordhinta.

Xuduudaha Kit

Codsiga: noocyo kala duwan oo unugyo xayawaan ah.

DNA-da laga sii daayay muunada lysis: kaliya waxaa loo adeegsaday qaab PCR.

Qalabka waxaa loo isticmaali karaa ujeedooyinka soo socda: aqoonsiga transgenosis, genotyping xayawaanka, iwm.

Socodka shaqada

wk flw-noolaha unugyada tooska ah PCR

Kaydinta iyo nolosha shelf

Qaybta I ee xirmadan waa in lagu kaydiyaa 2-8℃.

Reagent Buffer AL waxa lagu kaydin karaa 12 bilood xaaladaha qalalan;waxa lagu kaydin karaa 2-8℃ kaydinta dheer

Reagent Foregene Protease wuxuu leeyahay qaacido gaar ah.Si loo hubiyo dhaqdhaqaaqeeda iyo xasiloonideeda, fadlan ku kaydi at 4℃ ilaa 12 bilood.

Reagent 6×DNA Loading Buffer waxa lagu kaydin karaa 4℃ ama -20℃ wakhti dheer.

Qaybta II ee xirmadan waa in lagu kaydiyaa -20℃.

Reagent 2× PCR Fudud^TMMix (UNG), haddii si joogto ah loo isticmaalo, waxa kale oo lagu kaydin karaa at 4 ℃ kaydinta muddada-gaaban (isticmaal ilaa 10 maalmood gudahood) .Waxaan ku dheggannahay mabda'a ah "tayada marka hore, adeegga ugu horreeya, horumarinta joogtada ah iyo hal-abuurka si ay ula kulmaan macaamiisha" ee maamulka iyo" eber ciladaha, eber cabashooyinka" sida ujeedada tayada.Si aan u dhamaystirno adeegeena, waxaanu ku siinaynaa alaabta tayada wanaagsan ee qiimaha macquulka ah ee Qeexida Sare ee Shiinaha Nucleic Acid Sifeynta Soo saarista Yc-701 Cov Nucleic Acid, Xoog saarista khaaska ah ee ku saabsan baakadaha badeecada si looga fogaado waxyeelo kasta inta lagu jiro gaadiidka.
Qeexitaan sareNidaamka Soo saarista Ashitada Nukliyeerka ee Shiinaha, Qalabka Soosaarka Asiidhka Nucleic, Waxaan si sii kordheysa u ballaarineynaa saamigayaga suuqa caalamiga ah iyadoo ku saleysan alaab tayo leh, adeeg heer sare ah, qiimo macquul ah iyo gaarsiinta waqtiga.Fadlan nala soo xidhiidh wakhti kasta wixii macluumaad dheeraad ah.

Hore: Xirmada Soo saarista Ashitada Nucleic ee Fayraska Sare ee Shiinaha, Sanduuqa Ashitada Nukliyeerka

Xiga: Qiimaha macquulka ah ee Shiinaha-waqtiga dhabta ah ee Fluorescence Virus Nucleic Acid PCR Test

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