Shiinaha Tayada Sare ee Shiinaha PCR Tusaalaha Diyaarinta Ashitada Nukliyeerka Si Toos ah Soo Saare iyo Soo-saare |Foregene

Tayada Sare ee Shiinaha PCR Tusaalaha Diyaarinta Si Toos ah Soo Saarista Ashitada Nukliyeerka

Tayada Sare ee Shiinaha PCR Tusaalaha Diyaarinta Toomaatigga ah ee Ashitada Nadiifinta Nukliyeerka Sawirka

Sharaxaada Qalabka:

Qalabkani wuxuu isticmaalaa Real-time RT PCR technology (rRT-PCR) si loo ogaado tayada SARS-CoV-2 (ORF1ab hiddo iyo N gene) acids nucleic in nasopharyngeal bini'aadamka ama oropharyngeal swab muunado, by multiplex PCR fluorescent probe method. Tani xirmo ka kooban kontoroolka gudaha si loo qiimeeyo shaybaarka baahida shaybaarka ee tayada Nuclearka, sidaas darteed ma dhamaystiri karo baahida saafiga ah ee aashitada. ogaanshaha degdega ah.

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Faahfaahinta Alaabta

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Helitaanka buuxinta iibsadaha waa ujeedada shirkadeena aan dhamaadka lahayn.Waxaan samayn doonaa dadaallo aad u fiican si aan u helno xalal cusub oo tayo sare leh, la kulanno tafaasiishaada gaarka ah oo aan ku siino iibka hore, iibinta iyo bixiyeyaasha iibka ka dib ee Tayada Sare ee Shiinaha PCR Tusaalaha Diyaarinta Qalabka Nucleic Acid Nadiifinta, Tayada sare, adeegyada waqtiga iyo qiimaha iibinta gardarrada ah, dhamaantood waxay nagu helayaan caan ka sarreeya garoonka xxx ee caalamiga ah.
Helitaanka buuxinta iibsadaha waa ujeedada shirkadeena aan dhamaadka lahayn.Waxaan samayn doonaa dadaallo aad u fiican si aan u helno xalal cusub oo tayo sare leh, oo aan la kulano tafaasiishaada gaarka ah oo aan ku siin doono iibka kahor, iibka iyo bixiyeyaasha iibka kadibHeerkulbeegga Infrared ee Shiinaha, Qalabka-Sayniska Nolosha, Waxaan hadda leenahay 8 sano oo waayo-aragnimo ah wax soo saarka iyo 5 sano oo waayo-aragnimo ah ganacsiga macaamiisha adduunka oo dhan.macaamiisheena inta badan waxaa lagu qaybiyaa Waqooyiga Ameerika, Afrika iyo Bariga Yurub.Waxaan ku siin karnaa alaab tayo sare leh oo leh qiimo aad u tartan badan.

Tilmaamaha

48 Rxns, 96 Rxns

Maya	Qayb	Qadarka				Qaybaha ugu muhiimsan
Maya	Qayb	48 Rxns		96 Rxns		Qaybaha ugu muhiimsan
1	Wakiilka sii daaya acid nucleic	1.4ml/tubo	2 tuubbo	5.3 ml/ dhalo	1 dhalo	Surfactant
2	difaaca RNA	27 μL/tubo	1 tube	53 μL/tubo	1 tube	RNase inhibitor
3	Xalka falcelinta SARS-CoV-2	800 μL/tubo	1 tube	1600 μL/tubo	1 tube	Fure, baadhitaan, falcelin celin, dNTP
4	Isku dhafka enzyme SARS-CoV-2	80 μL/tubo	1 tube	160 μL/tubo	1 tube	Bilawga kulul ee Taq enzyme, M-MLV enzyme
5	SARS-CoV-2 xakamaynta togan	100 μL/tubo	1 tube	100 μL/tubo	1 tube	Plasmid dib-u-habayn oo ka kooban jajab bartilmaameed, RNA
6	SARS-CoV-2 xakamaynta taban	1200 μL/tubo	1 tube	1200 μL/tubo	1 tube	TE baffer

Tilmaamaha & faa'iidooyinka

Degdeg ah oo fudud.
Iska ilaali luminta aashitada nucleic.
Xadka ogaanshaha hooseeya iyo dareenka sare.
Qalabka loo baahan yahay oo hooseeya iyo isticmaalka aadka u dabacsan.

Dareenka ogaanshaha wuxuu gaari karaa heerka ugu hooseeya sida 500 koobi/ml).

GCE/ml	Qiimaha Ct
GCE/ml	N hiddo-wadaha	ORF1ab hidde
2.00E+08	16.43	15.16
2.00E+07	19.48	18.58
2.00E+06	22.71	21.86
2.00E+05	25.95	25.33
2.00E+04	29.30	28.49
2.00E+03	32.37	31.37
2.00E+02	35.32	34.47
2.00E+01	38.29	38.27
2.00E+00	N/A	N/A
2.00E-01	N/A	N/A
NC	N/A	N/A

qalooca dhawaaqa toosan

qalooca xoojinta-RT-qPCR

Sida loo isticmaalo

1. Qalab lagu dabaqi karo

xirmada ogaanshaha covid-19 nucleic acid

2. Habka ogaanshaha

xirmada ogaanshaha covid-19 nucleic acid

Samaynta Tusaalaha: Muunada suufka naafeeya ama oropharyngeal (kaliya 1 tillaabo hawlgal) (Sug 10 daqiiqo)

PCR amplificaton (<= 55min)

Socodka shaqada:

xirmada ogaanshaha covid-19 nucleic acid 4

Kaydinta

laga xidhay iftiinka oo lagu kaydiyay -20 ± 5 ℃;

kaydinta
kaydinta2

Nolosha shelf:1 sano

Helitaanka buuxinta iibsadaha waa ujeedada shirkadeena aan dhamaadka lahayn.Waxaan samayn doonaa dadaallo aad u fiican si aan u helno xalal cusub oo tayo sare leh, la kulanno tafaasiishaada gaarka ah oo aan ku siino iibka hore, iibinta iyo bixiyeyaasha iibka ka dib ee Tayada Sare ee Shiinaha PCR Tusaalaha Diyaarinta Qalabka Nucleic Acid Nadiifinta, Tayada sare, adeegyada waqtiga iyo qiimaha iibinta gardarrada ah, dhamaantood waxay nagu helayaan caan ka sarreeya garoonka xxx ee caalamiga ah.
Tayo sare oo loogu talagalayHeerkulbeegga Infrared ee Shiinaha, Qalabka-Sayniska Nolosha, Waxaan hadda leenahay 8 sano oo waayo-aragnimo ah wax soo saarka iyo 5 sano oo waayo-aragnimo ah ganacsiga macaamiisha adduunka oo dhan.macaamiisheena inta badan waxaa lagu qaybiyaa Waqooyiga Ameerika, Afrika iyo Bariga Yurub.Waxaan ku siin karnaa alaab tayo sare leh oo leh qiimo aad u tartan badan.

Hore: Qalabka Tijaabada Asiidhka Nukliyeerka ee Shiinaha ee Rna

Xiga: Xirmooyinka Ogaanshaha Tuujin ee Warshada Shiinaha Techstar Tusaalaha Siideynta Reagent Baahnayn Rna/Qalabka Soosaarida Asiidhka Nukliyeerka ah ee Xirmooyinka Wakhtiga Dhabta ah

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