RT Easyᵀᴹ I Master Premix-ka ugu horreeya ee cDNA-yada
Sharaxaada Qalabka:
-Nidaamka dib-u-dejinta oo hufan, kaliya waxay qaadataa 25 daqiiqo in la dhammaystiro isku-darka cDNA-ga ugu horreeya.
- Nidaamka qoraalka dib u celinta dareenka sare, jaantusyada heerka pg sidoo kale waxay heli karaan cDNA tayo sare leh.
- Nidaamka qoraalka dib-u-celinta wuxuu leeyahay xasillooni heerkul sare ah, heerkulka falcelinta nidaamku wuxuu noqon karaa mid sarreeya 50 ℃,waxayna leedahay wax qabad wanaagsan oo qoraal ah.
- Xataa muunadaha RNA ee aan nadiifka ahayn (khamriga ilaa 45%, cusbada guanidine ilaa 750mM) ayaa lagu soo rogi karaa falcelin qoraal ah.
Sharaxaada Kit
FOREGENE's 2× RT EasyTMIsku-darku waxa uu isku-dubbari karaa cDNA-da-koowaad heerkulkiisu gaadhayo 50°C, kaas oo ku habboon falcelinta qoraalka qoraalka ee qaab-dhismeedka sare ee RNA.
RNA-da lagu sifeeyay shaybaadhka inta badan waxa ku jira aalkolo iyo hadhaaga cusbada guanidine, kuwaas oo leh sifooyin adag oo ka hortagaya inta badan qoraalada gadaasha, taas oo keenta saameyn qoraal ah oo aan fiicnayn ama waxtarka qoraalka gadaasha oo hooseeya.Foregene 2× RT FududTMIsku darka ayaa muujinaya dulqaad aad u sarreeya oo khamriga iyo milixda guanidine, dulqaadka ugu sarreeya ee khamriga ee muunadaha RNA waa 45%, iyo dulqaadka ugu sarreeya ee cusbada guanidine waa 750mM.Xataa haddii RNA aanu saafi ahayn, falcelinta qoraalka dib u noqoshada waxa lagu samayn karaa FORREGENE 2×RT Mix.Habka falcelinta gaarka ah ayaa ka dhigaysa falcelinta RT mid sahlan, dhakhso badan oo hufan, iyo isku dhafka cDNA-ga ugu horreeya waxa lagu dhamayn karaa 25 daqiiqo.
Alaabtan waxa loo isticmaali karaa iyada oo lala kaashanayo Foregene's fluorescent shayga qiyaaseed ee Real Time PCR EasyTMsi loo helo natiijooyin tijaabo ah oo tayo sare leh.
Tilmaamaha
| RT FududTMI Master Premix oo loogu talagalay isku dhafka cDNA-xarun-koowaad | ||
| Qalabka ku jira | RT-01011 | RT-01012 |
| 25 jeer (Nidaamka 20 μl) | 100 jeer (Nidaamka 20μl) | |
| 2× RT EasyTM Mix | 0.25ml | 1ml |
| Random Primer (50μM) | 50ml | 200ml |
| Oligo(dT)18 Primer (50μM) | 50ml | 200ml |
| RNase-free ddH2O | 1.7ml | 1.7ml |
| Tilmaamaha | 1 gabal | 1 gabal |
Tilmaamaha & faa'iidooyinka
-Nidaamka dib-u-dejinta oo hufan, kaliya waxay qaadataa 25 daqiiqo in la dhammaystiro isku-darka cDNA-ga ugu horreeya.
- Nidaamka qoraalka dib u celinta dareenka sare, jaantusyada heerka pg sidoo kale waxay heli karaan cDNA tayo sare leh.
- Nidaamka qoraalka dib u celinta wuxuu leeyahay xasillooni kuleyl sare leh, heerkulka falcelinta nidaamku wuxuu noqon karaa mid aad u sarreeya 50 ℃, wuxuuna leeyahay waxqabad qoraal ah oo wanaagsan.
- Xataa muunadaha RNA ee aan nadiifka ahayn (khamriga ilaa 45%, cusbada guanidine ilaa 750mM) ayaa lagu soo rogi karaa falcelin qoraal ah.
Codsiga qalabka
- RT-PCR-ga caadiga ah
- Synthesis of cDNA for cloning and expression research
- Falanqaynta habka fidinta hore ee goobta qoraalka qoraalka
- RACE (kordhinta degdega ah ee cDNA way dhammaanaysaa)
- Kordhinta RNA toosan
- Calaamadda Chip
Socodka shaqada
Kaydinta iyo nolosha shelf
1.Xaaladaha gaadiidka
Geedi socodka oo dhan ee sanduuqa barafka heerkulka hooseeya, si loo hubiyo in qalabku ku jiro xaalad <4 ℃ ah.
2. Xaaladaha kaydinta
RT FududTMWaxa la igu kaydiyaa -20°C.Ku kaydi alaabta qaboojiyaha heerkulka joogtada ah -20 ° C isla markiiba ka dib markii la helo.Haddii shuruudaha kaydinta ay ku habboon yihiin, alaabtu ma dhimi doonto wax-qabad kasta inta lagu jiro muddada ansaxinta 1-sano.
