Baadhitaanka Tayada Shiinaha ee Shiinaha ee Tayada Sare ee Hiddaha ee Tijaabada Qalabka Soo-ururinta Candhuufta, DNA Rna Fayraska Tijaabada Shaybaarka Hidde-sidayaasha La Tuuri Karo Soo Saaraha iyo Iibiyaha |Foregene

Baadhitaanka Tayada Shiinaha ee Tayada Sare ee Hidde-sidayaasha ee Tijaabada Qalabka Soo-ururinta Candhuufta, DNA Rna Fayraska Tijaabada Shaybaarka Hiddaha la Tuuri karo

Baadhitaanka Tayada Shiinaha ee Tayada Sare ee Hidde ee Tijaabada Qalabka Soo-ururinta Candhuufta, DNA Rna Fayraska Baadhista shaybaadhka Hiddaha la Tuuri karo

Sharaxaada Qalabka:

Qalabkani wuxuu isticmaalaa Real-time RT PCR technology (rRT-PCR) si loo ogaado tayada SARS-CoV-2 (ORF1ab hiddo iyo N gene) acids nucleic in nasopharyngeal bini'aadamka ama oropharyngeal swab muunado, by multiplex PCR fluorescent probe method. Tani xirmo ka kooban kontoroolka gudaha si loo qiimeeyo shaybaarka baahida shaybaarka ee tayada Nuclearka, sidaas darteed ma dhamaystiri karo baahida saafiga ah ee aashitada. ogaanshaha degdega ah.

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Faahfaahinta Alaabta

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Shaqaalaheenna waxaan u marnay tababar khibrad leh.Aqoonta khabiirka ah ee xirfadda leh, dareenka xoogga leh ee caawinta, si loo qanciyo baahida adeegga macaamiisha ee Kormeerka Tayada ee Shiinaha Tayada Sare ee Hiddaha Tijaabada Qalabka Soo-ururinta Candhuufta, Baaritaanka DNA Rna Fayraska Shaybaarka Hiddaha la Tuuri karo, Abuur xalal leh qiimaha sumadda.Waxaan si dhab ah uga qayb-galnaa si aan u soo saarno oo aan ugu dhaqanno si daacad ah, iyo sababta oo ah raallinimada macaamiisha gurigaaga iyo dibedda ee warshadaha xxx.
Shaqaalaheenna waxaan u marnay tababar khibrad leh.Aqoon khabiir xirfad leh, dareen xoog leh oo gargaar, si loo qanciyo baahida adeegga macaamiishaShiinaha Candhuufta la tuuri karo, Qaadiyaha Imtixaanka Candhuufta, Shirkaddayadu waxay ku adkaysanaysaa ujeedada "waxay qaadataa mudnaanta adeegga heerka, dammaanadda tayada ee astaanta, ganacsi u samee si daacad ah, si aad u bixiso adeeg tayo leh, degdeg ah, sax ah iyo waqti kugu habboon".Waxaan soo dhawaynaynaa macaamiishii hore iyo kuwa cusub inay nala xaajoodaan.Waxaan kuugu adeegi doonaa si daacad ah!

Tilmaamaha

48 Rxns, 96 Rxns

Maya	Qayb	Qadarka				Qaybaha ugu muhiimsan
Maya	Qayb	48 Rxns		96 Rxns		Qaybaha ugu muhiimsan
1	Wakiilka sii daaya acid nucleic	1.4ml/tubo	2 tuubbo	5.3 ml/ dhalo	1 dhalo	Surfactant
2	difaaca RNA	27 μL/tubo	1 tube	53 μL/tubo	1 tube	RNase inhibitor
3	Xalka falcelinta SARS-CoV-2	800 μL/tubo	1 tube	1600 μL/tubo	1 tube	Fure, baadhitaan, falcelin celin, dNTP
4	Isku dhafka enzyme SARS-CoV-2	80 μL/tubo	1 tube	160 μL/tubo	1 tube	Bilawga kulul ee Taq enzyme, M-MLV enzyme
5	SARS-CoV-2 xakamaynta togan	100 μL/tubo	1 tube	100 μL/tubo	1 tube	Plasmid dib-u-habayn oo ka kooban jajab bartilmaameed, RNA
6	SARS-CoV-2 xakamaynta taban	1200 μL/tubo	1 tube	1200 μL/tubo	1 tube	TE baffer

Tilmaamaha & faa'iidooyinka

Degdeg ah oo fudud.
Iska ilaali luminta aashitada nucleic.
Xadka ogaanshaha hooseeya iyo dareenka sare.
Qalabka loo baahan yahay oo hooseeya iyo isticmaalka aadka u dabacsan.

Dareenka ogaanshaha wuxuu gaari karaa heerka ugu hooseeya sida 500 koobi/ml).

GCE/ml	Qiimaha Ct
GCE/ml	N hiddo-wadaha	ORF1ab hidde
2.00E+08	16.43	15.16
2.00E+07	19.48	18.58
2.00E+06	22.71	21.86
2.00E+05	25.95	25.33
2.00E+04	29.30	28.49
2.00E+03	32.37	31.37
2.00E+02	35.32	34.47
2.00E+01	38.29	38.27
2.00E+00	N/A	N/A
2.00E-01	N/A	N/A
NC	N/A	N/A

qalooca dhawaaqa toosan

qalooca xoojinta-RT-qPCR

Sida loo isticmaalo

1. Qalab lagu dabaqi karo

xirmada ogaanshaha covid-19 nucleic acid

2. Habka ogaanshaha

xirmada ogaanshaha covid-19 nucleic acid

Samaynta Tusaalaha: Muunada suufka naafeeya ama oropharyngeal (kaliya 1 tillaabo hawlgal) (Sug 10 daqiiqo)

PCR amplificaton (<= 55min)

Socodka shaqada:

xirmada ogaanshaha covid-19 nucleic acid 4

Kaydinta

laga xidhay iftiinka oo lagu kaydiyay -20 ± 5 ℃;

kaydinta
kaydinta2

Nolosha shelf:1 sano

Shaqaalaheenna waxaan u marnay tababar khibrad leh.Aqoonta khabiirka ah ee xirfadda leh, dareenka xoogga leh ee caawinta, si loo qanciyo baahida adeegga macaamiisha ee Kormeerka Tayada ee Shiinaha Tayada Sare ee Hiddaha Tijaabada Qalabka Soo-ururinta Candhuufta, Baaritaanka DNA Rna Fayraska Shaybaarka Hiddaha la Tuuri karo, Abuur xalal leh qiimaha sumadda.Waxaan si dhab ah uga qayb-galnaa si aan u soo saarno oo aan ugu dhaqanno si daacad ah, iyo sababta oo ah raallinimada macaamiisha gurigaaga iyo dibedda ee warshadaha xxx.
Kormeerka Tayada ee Shiinaha Soo-qaadiyaha Candhuufta la tuuri karo, Qaadiyaha Imtixaanka Candhuufta, Shirkaddayadu waxay ku adkaysanaysaa ujeedada "waxay qaadataa mudnaanta adeegga heerka, dammaanadda tayada ee astaanta, ganacsi u samee si daacad ah, si aad u bixiso adeeg tayo leh, degdeg ah, sax ah iyo waqti kugu habboon".Waxaan soo dhawaynaynaa macaamiishii hore iyo kuwa cusub inay nala xaajoodaan.Waxaan kuugu adeegi doonaa si daacad ah!

Hore: Soo saaraha ODM Shiinaha Scs-Su Tukadaha Ururinta Swab Swab Kit Vtm Kit

Xiga: Iibka Sare ee Shiinaha Rna Rn112 Qalabka Tijaabada Degdegga ah ee Baadhista Kitextraction, Xirmada Soo Saaridda Asiidhka Nukliyeerka ee Rna & Qalabka Sifaynta DNA

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