Shaybaadhka Shiinaha ee ODM ee Jumladda Isticmaalka Baaskiil-kulka Ku-salaysan PCR
Ururku wuxuu ilaaliyaa fikradda nidaamka "Maamulka sayniska, tayada sare leh iyo mudnaanta koowaad ee wax ku oolnimada, wax iibsiga ugu sarreeya ee Shaybaarka Shiinaha ee ODM Isticmaalka Peltier-BasedWareegtada kulaylkaPCR, Haddii loo baahdo, ku soo dhawoow inaad nagula soo xiriirto boggayaga shabakadda ama la talinta taleefanka, waxaan ku farxi doonnaa inaan ku adeegno.
Ururku wuxuu ilaaliyaa habka fikradda "Maamulka sayniska, tayada sare leh iyo mudnaanta waxtarka leh, ugu sarreeya ee wax iibsigaShiinaha PCR, Wareegtada kulaylka, Isbeddellada isbeddelka ee goobtan awgeed, waxaan nafteena ku lug yeelaneynaa ka ganacsiga badeecadaha iyadoo la adeegsanayo dadaallo heegan ah iyo heer sare maamul.Waxaan ilaalinaa jadwalada gaarsiinta waqtiga, naqshadaha cusub, tayada iyo daahfurnaanta macaamiisheena.Hal-ku-dheggeenu waa in aanu ku soo bandhigno xalal tayo leh wakhtiga loo cayimay.
Tilmaamaha
48 Rxns, 96 Rxns
Maya | Qayb | Qadarka | Qaybaha ugu muhiimsan | |||
48 Rxns | 96 Rxns | |||||
1 | Wakiilka sii daaya acid nucleic | 1.4ml/tubo | 2 tuubbo | 5.3 ml/ dhalo | 1 dhalo | Surfactant |
2 | difaaca RNA | 27 μL/tubo | 1 tube | 53 μL/tubo | 1 tube | RNase inhibitor |
3 | Xalka falcelinta SARS-CoV-2 | 800 μL/tubo | 1 tube | 1600 μL/tubo | 1 tube | Fure, baadhitaan, falcelin celin, dNTP |
4 | Isku dhafka enzyme SARS-CoV-2 | 80 μL/tubo | 1 tube | 160 μL/tubo | 1 tube | Bilawga kulul ee Taq enzyme, M-MLV enzyme |
5 | SARS-CoV-2 xakamaynta togan | 100 μL/tubo | 1 tube | 100 μL/tubo | 1 tube | Plasmid dib-u-habayn oo ka kooban jajab bartilmaameed, RNA |
6 | SARS-CoV-2 xakamaynta taban | 1200 μL/tubo | 1 tube | 1200 μL/tubo | 1 tube | TE baffer |
Tilmaamaha & faa'iidooyinka
Degdeg ah oo fudud.
Iska ilaali luminta aashitada nucleic.
Xadka ogaanshaha hooseeya iyo dareenka sare.
Qalabka loo baahan yahay oo hooseeya iyo isticmaalka aadka u dabacsan.
Dareenka ogaanshaha wuxuu gaari karaa heerka ugu hooseeya sida 500 koobi/ml).
GCE/ml | Qiimaha Ct | |
N hiddo-wadaha | ORF1ab hidde | |
2.00E+08 | 16.43 | 15.16 |
2.00E+07 | 19.48 | 18.58 |
2.00E+06 | 22.71 | 21.86 |
2.00E+05 | 25.95 | 25.33 |
2.00E+04 | 29.30 | 28.49 |
2.00E+03 | 32.37 | 31.37 |
2.00E+02 | 35.32 | 34.47 |
2.00E+01 | 38.29 | 38.27 |
2.00E+00 | N/A | N/A |
2.00E-01 | N/A | N/A |
NC | N/A | N/A |
qalooca dhawaaqa toosan
Sida loo isticmaalo
1. Qalab lagu dabaqi karo
2. Habka ogaanshaha
Samaynta Tusaalaha: Muunada suufka naafeeya ama oropharyngeal (kaliya 1 tillaabo hawlgal) (Sug 10 daqiiqo)
PCR amplificaton (<= 55min)
Socodka shaqada:
Kaydinta
laga xidhay iftiinka oo lagu kaydiyay -20 ± 5 ℃;
Nolosha shelf:1 sanoUrurku wuxuu hayaa fikradda nidaamka "Maamulka sayniska, tayada sare iyo mudnaanta koowaad ee wax ku oolnimada, dukaanka ugu sarreeya ee Jumlada ODM Shiinaha Shaybaarka Isticmaalka Peltier-Based Thermal Cycler PCR, Haddii loo baahdo, ku soo dhawoow inaad nala soo xiriirto boggayaga internetka ama la-tashiga telefoonka, waxaan ku farxi doonaa inaan kuu adeegno.
Jumlada ODMShiinaha PCR, Baaskiilada kulaylka, Sababo la xiriira isbeddellada isbeddelka ee goobtan, waxaan ku lug yeelanay nafteena ganacsiga badeecada dadaal gaar ah iyo maamul heer sare ah.Waxaan ilaalinaa jadwalada gaarsiinta waqtiga, naqshadaha cusub, tayada iyo daahfurnaanta macaamiisheena.Hal-ku-dheggeenu waa in aanu ku soo bandhigno xalal tayo leh wakhtiga loo cayimay.