Jumlada OEM Shiinaha 2 kanaalka-Waqtiga-dhabta ah ee Tirooyinka PCR Kulayliyaha ee leh Ce
Sharaxaada Qalabka:
- Xidhmada hal-tallaabo ah waxay samaysaa qoraal-celin iyo qPCR laba falcelin oo isku mid ah tubbada, kaliya waxay u baahan yihiin in lagu daro template RNA, aasaasayaasha PCR gaar ah iyo RNase-free ddH2O.
- Xirmada ayaa si degdeg ah oo hufan u baari karta RNA fayraska ama raadraaca RNA.
- Xidhmadani waxay isticmaashaa reagent transcription u gaar ah Foregene iyo Foregene HotStar Taq DNA Polymerase oo ay weheliso nidaam falcelin gaar ah si ay si wax ku ool ah u wanaajiso waxtarka xoojinta iyo gaar ahaaneed ee falcelinta.
-Nidaamka falcelinta ee la wanaajiyey ayaa ka dhigaysa fal-celintu inay yeelato dareen ogaansho sare leh, degganaansho kulayleed oo xoog badan, iyo dulqaad wanaagsan.
- RT-qPCR FududTM(Hal Tallaabo) -SYBR Cagaaran I xirmada waxay la socotaa ROX dheeha tixraaca gudaha, kaas oo loo isticmaali karo in lagu baabi'iyo asalka calaamadaha iyo khaladaadka u dhexeeya ceelasha, taas oo ku habboon macaamiisha inay u isticmaalaan noocyo kala duwan oo qalab PCR ah.
Waxaan ballan qaadnay inaan bixino qiimaha tartanka, alaabada ugu fiican iyo xalalka tayada sare leh, isla markaana si dhakhso leh loogu keeno Jumlo OEM China 2 Channels Real-Time Quantitative PCR Thermal Cycler oo leh Ce, heer aad u fiican, qiimaha iibinta tartanka, gaarsiinta degdega ah iyo bixiye la isku hallayn karo ayaa dammaanad qaadaya si naxariis leh noo ogeysii baahidaada tirada si waafaqsan qayb kasta.
Waxaa naga go'an inaan bixino qiimaha tartanka, alaabada ugu fiican iyo xalalka tayada sare leh, isla markaana si dhakhso leh loo gaarsiinayoShiinaha PCR, Mashiinka PCr, Waxaan raadineynaa fursado aan kula kulanno dhammaan asxaabta gudaha iyo dibaddaba si loo helo iskaashi guul-guuleysi leh.Waxaan si dhab ah u rajaynaynaa in aan dhammaantiin la yeelano iskaashi fog oo ku salaysan faa'iido wadaag iyo horumar guud.
Tilmaamaha
50×20µl rxns, 200×20µl rxns, 500×20µl rxns, 2000×20µl rxns
Qaybaha kitka
| Qaybta I | Buffer P1 |
| Buffer P2 | |
| 6×DNA Loading Buffer | |
| Qaybta II | 2× Caleen PCR FududTMIsku qas(UNG) |
Tilmaamaha & faa'iidooyinka
■ Ma jiro waqti-qaadasho iyo nadiifin DNA-da qaali ah
■ Waxyaabo yar
■ Fudud-muunada waxaa lagu soo rogi karaa falcelinta PCR ama falcelinta lysis iyada oo aan la jarin ama la shiidi
■ 2 × Isku qas, yaraynta khaladaadka muunada rarida iyo wakhtiga diyaarinta nidaamka falcelinta
■ Diyaarinta qaab-dhaqso leh ayaa lagu dhammeyn karaa 10 daqiiqo, falcelinta PCR waxay ku dhameyn kartaa ugu yaraan 50 daqiiqo
■ Dareen-celinta-lysis-ka sare ee fal-celinta waxaa lagu dhammayn karaa saxan PCR 96-wanaagsan
Xuduudaha Kit
Codsiga: Aqoonsiga hidde ahaan la beddelay, genotyping, iwm.
Tusaale: caleen geed
Qiyaasta: Dhexroorka 2-3mm (hab toos ah), dhexroor 5-7mm (qaabka lysis)
Qiyaasta ogaanshaha: jajabka bartilmaameedka ≤1kb
Socodka shaqada
Electroferogram
Laba toddobaad oo natiijooyinka ogaanshaha PCR Mix ee hoos yimaada xaaladda PCR amplification product contamination.We have been go'aan si ay u bixiyaan qiimaha tartanka, alaabta heer sare ah iyo xal tayo sare leh, isla mar ahaantaana sida degdega ah u gaarsiinta OEM China 2 Channels Real-Time Tire PCR kulaylka Cycler la Ce, heer sare ah, heer sare ah iibka bixiyaha si deg deg ah u baahan tahay in la ogaado qiimaha iibka ee nooc kasta, waanu ku soo wargelin doonaa.
OEM JumladdaShiinaha PCR, PCR Machine, Waxaan raadineynaa fursad aan kula kulanno dhammaan asxaabta gudaha iyo dibaddaba si loo helo wadashaqeyn guul-guul ah.Waxaan si dhab ah u rajaynaynaa in aan dhammaantiin la yeelano iskaashi fog oo ku salaysan faa'iido wadaag iyo horumar guud.
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