Xirmada Ashitada Nukliyeerka ah ee SARS-CoV-2 (Habka Baaritaanka Fluorescent PCR Multiplex)
Sharaxaada Qalabka:
PCR-ga tooska ah:Looma baahna xirmada soo saarista aashitada nucleic-ka iyo Nidaamka, oo la dhameeyay 96 muunado 1 saac ama wax ka badan.
◮ Qalabka loo baahan yahay oo hooseeya iyo isticmaalka aadka u dabacsan:Kaliya waxaad u baahan tahay nidaamka PCR-waqtiga dhabta ah.
◮ LoD iyo dareen sare:Dareenka ogaanshaha wuxuu gaari karaa heerka ugu hooseeya sida 500 nuqul/ml.
Isticmaalka la damacsan yahay
Mabda'a imtixaanka
Qalabkan asaasiga ah iyo qalabka baaritaanka waxaa loogu talagalay in lagu ogaado taxanaha la keydiyay ee SARS-CoV-2 (ORF1ab hiddo-wadaha, hidda-wadaha N iyo hiddaha E).Qalabka waxaa ku jira Kontorool Gudaha ah oo loo isticmaalo in lagu qiimeeyo tayada muunada.Tiknoolajiyada PCR-da-waqtiga-dhabta ah waxay u isticmaashaa falcelinta silsiladda polymerase (PCR) si ay u xoojiso taxanaha bartilmaameedka gaarka ah iyo bartilmaameedka baaritaanno gaar ah oo lagu ogaanayo RNA la xoojiyay.Baarayaasha waxaa lagu calaamadeeyay weriyaha fluorescent iyo midabada demiya.
Faahfaahin Deg Deg ah
◮Tilmaamaha:48 Rxns/kit, 96 Rxns/kit
Gaar ahaan:96.72%
Dareenka97.92%
Qaybaha
| Maya | Qayb | Qadarka | Walxaha ugu muhiimsan | |||
| 48 Rxns | 96 Rxns | |||||
| 1 | Nucleic acid sii daayowakiil | 1.5 ml/tubo | 1 tube | 1.5 ml/tubo | 1 tube | Surfactant |
| 2 | difaaca RNA | 27 μL/tubo | 1 tube | 53 μL/tubo | 1 tube | RNase inhibitor |
| 3 | SARS-CoV-2xal falcelin | 800 μL/tubo | 1 tube | 1600 μL/tubo | 1 tube | Fure, baadhitaan, falcelin celin, dNTP |
| 4 | SARS-CoV-2isku dhafka enzyme | 80 μL/tubo | 1 tube | 160 μL/tubo | 1 tube | Bilawga kulul ee Taq enzyme,M-MLV enzyme |
| 5 | SARS-CoV-2xakamaynta togan | 100 μL/tubo | 1 tube | 100 μL/tubo | 1 tube | Dib-u-habaynta plasmid ka kooban bartilmaameedjajab, RNA |
| 6 | SARS-CoV-2xakamaynta xun | 1200 μL/tubo | 1 tube | 1200 μL/tubo | 1 tube | TE baffer |
Tilmaamaha & faa'iidooyinka
Sida loo isticmaalo
Habka 1: Habka tooska ah ee PCR
Habka 2: Go'doominta Viral RNA + PCR
Habka 1: Habka tooska ah ee PCR adoo isticmaalayaTusaalaha Wakiilka Siideynta
Habka ogaanshaha
Gaadiidka Silsiladda Qabow iyo Kaydinta
Laga xidhay iftiinka oo lagu kaydiyay -20 ± 5 ℃;
Nolosha shelf
1 sano
Dalbashada Macluumaadka
