Xirmada Go'doominta MiRNA ee Xayawaanka Soo saarista iyo Xirmooyinka Sifaynta ee Xoolaha
Sharaxaada Qalabka:
Si dhakhso leh oo hufan uga soo saar jajabyada RNA yaryar ee 20-200nt miRNA, siRNA, snRNA ee unugyada iyo unugyada kala duwan ee xayawaanka.
◆Habka oo dhan waxaa lagu maamulaa heerkulka qolka (15-25°C), iyada oo aan lahayn qubeys baraf ah iyo heerkul hooseeya.
◆Xidhmada dhamaystiran ee RNase-Free, looma baahna in laga walwalo xaalufka RNA.
◆Tiirka Nadiifinta DNA-da ayaa si gaar ah u xidha DNA-da, si uu xidhmoku uga saaro wasakhaynta DNA-da iyada oo aan lagu darin DNA-da dheeraad ah.
◆Wax-soosaarka RNA sare: Tiirka-RNA-kaliya iyo qaacido gaar ah ayaa si hufan u nadiifin kara RNA.
◆Xawaaraha dheereeya: Qalliinku waa sahlan yahay waxaana lagu dhamayn karaa 30 daqiiqo gudahood.
◆Badbaadada: soo saarista reagents organic looma baahna.
◆Tayo sare leh: Qaybaha RNA ee la soo saaray waa nadiif sare, ka madax banaan borotiin iyo wasakh kale, waxayna buuxin karaan shuruudaha hoose.
◆Codsiyada tijaabada ah ee kala duwan
Sharaxaada
Xirmadani waxay isticmaashaa tiirka lafdhabarta iyo caanaha ay soo saartay shirkadeena, kaas oo si hufan u soo saari kara jajabyo yaryar oo RNA ah sida 20-200nt miRNA, siRNA, snRNA ee unugyada iyo unugyada kala duwan ee xayawaanka.Qalabku wuxuu bixiyaa DNA-Cleaning Column, kaas oo si fudud u kala sooci kara supernatant-ka unugyada lysate oo ka saari kara DNA-da iyo RNA dheer ee muunada, taas oo fudud oo waqti-badbaadinaysa;Tiirka RNA-keliya wuxuu si hufan ugu xidhi karaa RNA oo leh qaacido gaar ah, Wuxuu farsamayn karaa tiro badan oo muunado isku mar ah.Nidaamka oo dhan waa RNase-free, si aan miRNA la safeeyey aan hoos loo dhigin;Buffer miRW1, Buffer miRW2 nidaamka dhaqidda kaydka, si RNA-ga la helay uu xor uga ahaado borotiinka, DNA-ga, ions, iyo wasakheynta isku dhafka dabiiciga ah.
Qaybaha kitka
| Go'doominta miRNA xayawaanka Kit | ||||
| Qalabka ku jira | RE-0101T | RE-01011 | RE-01012 | RE-01014 |
| 5 T | 50 T | 100T | 200T | |
| Kaydka miRL* | 3ml | 25ml | 50ml | 100ml |
| Buffer miRW1 | 4ml | 35ml | 70ml | 140ml |
| Buffer miRW2 | 2.4ml | 24ml | 48ml | 96ml |
| RNase-free ddH2O | 1.7ml | 10ml | 20ml | 40ml |
| Tiirka RNA-kaliya | 5 dhigaysa | 50dhigaysa | 100dhigaysa | 200dhigaysa |
| Tiirka-Nadiifinta DNA | 5 dhigaysa | 50dhigaysa | 100dhigaysa | 200dhigaysa |
| IFU | 1 gabal | 1 gabal | 1 gabal | 1 gabal |
Tilmaamaha & faa'iidooyinka
◆Habka oo dhan waxaa lagu maamulaa heerkulka qolka (15-25°C), iyada oo aan lahayn qubeys baraf ah iyo heerkul hooseeya.
◆Xidhmada dhamaystiran ee RNase-Free, looma baahna in laga walwalo xaalufka RNA.
◆Tiirka Nadiifinta DNA-da ayaa si gaar ah u xidha DNA-da, si uu xidhmoku uga saaro wasakhaynta DNA-da iyada oo aan lagu darin DNA-da dheeraad ah.
◆Wax-soosaarka RNA sare: Tiirka-RNA-kaliya iyo qaacido gaar ah ayaa si hufan u nadiifin kara RNA.
◆Xawaaraha dheereeya: Qalliinku waa sahlan yahay waxaana lagu dhamayn karaa 30 daqiiqo gudahood.
◆Badbaadada: soo saarista reagents organic looma baahna.
◆Tayo sare leh: Qaybaha RNA ee la soo saaray waa nadiif sare, ka madax banaan borotiin iyo wasakh kale, waxayna buuxin karaan shuruudaha hoose.
◆Codsiyada tijaabada ah ee kala duwan
Codsiga qalabka
Waxay ku habboon tahay soo saarista iyo nadiifinta miRNA laga bilaabo unugyo ama unugyo xayawaan oo cusub ama barafaysan.
Kaydinta iyo nolosha shelf
Qalabkan waxa lagu kaydin karaa 24 bilood xaaladaha qalalan ee heerkulka qolka15-25°C), haddii loo baahdo in lagu kaydiyo wakhti dheer, waxa lagu kaydin karaa2-8°C.Buffer miRL waxa lagu meelayn karaa4°C1 bil ka dib marka lagu daroβ-mercaptoethanol(waxaa lagu talinayaa in lagu daro hadda).
