Dhiiga SuperDirectᵀᴹ PCR Kit(UNG)-Heparin Blood Toos ah PCR Master Mix for Genotyping of Blood
Sharaxaada Qalabka:
Si toos ah u isticmaal heparin xinjirowga lidka ku ah dhiigga oo dhan tusaale ahaan u-qaadista PCR, oo leh dareen sare iyo hufnaanta sare-u-qaadista.Nidaamka ka hortagga wasakheynta ee UNG ayaa lagu daray si looga hortago waxyaabo been abuur ah.
◮Nidaamku wuxuu leeyahay awood xoog-weyneyn wuxuuna si xasaasi ah u ogaan karaa genome-ka iyo jajabyada DNA-da ee ajnabiga ah ee dhiigga ku jira.
◮2× Aad u FududTMMix(UNG)-U adkaysiga Heparin Dhiiga sare: ilaa 45% ee dhiiga bini'aadamka iyo 20% ee dhiiga jiirka.
◮Muunadda si buuxda ayaa loo xidhay, markaa looma baahna in laga welwelo wasakhowga muunadda iyo natiijooyinka PCR ee beenta ah.Nidaamka PCR-ka-hortagga wasakheynta
◮2×SuperEasyTMIsku qas (UNG), si wax ku ool ah u baabi'iso wasakhowga ay keenaan alaabada PCR, oo hubi gaar ahaan iyo saxnaanta kordhinta.
Tilmaamaha
200 × 20μl rxns, 2000 × 20μl rxns
2× Aad u FududTMMix(UNG)-Heparin: Iyada oo ku saleysan 2× SuperEasyTMMix-Heparin, dUTP waxaa loo isticmaalaa halkii dTTP, iyo UNG ensaymes oo hoos u dhigi kara template dUTP ayaa lagu daray isku mar, kaas oo si wax ku ool ah uga hortagi kara wasakheynta alaabta PCR.Ka kooban yahay D-Taq DNA Polymerase, dNTPs, MgCl2, falcelinta falcelinta, xoojisay falcelinta PCR, hagaajinta iyo xasiliyaha, iwm. Falcelinta PCR, kaliya ku dar dhiiga oo dhan ee xinjirowga lidka ku ah, alwaaxyada, iyo ddH2O ilaa 2 × SuperEasyTM Mix (UNG) u dhigma -Heparin loo isticmaalo falcelinta PCR.
6× Kaydinta Soodejinta DNA-da: Kaydka Soodejinaya kuma jiraan SDS.Waxaa lagu talinayaa in la isticmaalo 6 × DNA Loading Buffer oo ay ku jiraan xirmada marka la samaynayo agarose gel electrophoresis si loo helo natiijooyin elektrophoresis wanaagsan.Ha isticmaalin Loading Buffer ka kooban SDS, haddii kale waxaa jiri doona dabo weyn oo iftiin dhalaalaya haadka inta lagu jiro electrophoresis, taas oo saameyn doonta natiijooyinka tijaabada.
Qaybaha kitka
| 2× Aad u FududTMMix(UNG)-Heparin |
| 6× DNA Loading Buffer |
| Tilmaamaha |
Tilmaamaha & faa'iidooyinka
-Nidaamku wuxuu leeyahay awood xoog-weyneyn wuxuuna si xasaasi ah u ogaan karaa genome-ka iyo jajabyada DNA-da ee ajnabiga ah ee dhiigga ku jira.
-2× SuperEasyTMMix(UNG)-U adkaysiga Heparin Dhiiga sare: ilaa 45% ee dhiiga bini'aadamka iyo 20% ee dhiiga jiirka.
-Muunada si buuxda ayaa loo xidhay, markaa looma baahna in laga welwelo wasakhaynta muunadda iyo natiijooyinka PCR beenta ah.Nidaamka PCR-ka-hortagga wasakheynta 2×SuperEasyTMIsku qas (UNG), si wax ku ool ah u baabi'iso wasakhowga ay keenaan alaabada PCR, oo hubi gaar ahaan iyo saxnaanta kordhinta.
Codsiga qalabka
U heellan in si toos ah loo aqoonsado PCR ee dhiigga oo dhan ee Heparin xinjirowga lidka ku ah.( oo ay ku jiraan: dhiigga dadka, dhiigga jiirka, dhiigga digaagga, dhiigga shimbiraha, dhiigga lo'da, dhiigga eyga, iwm.)
Socodka shaqada
Kaydinta iyo nolosha shelf
Geedi socodka oo dhan ee sanduuqa barafka heerkulka hooseeya, si loo hubiyo in qalabku ku jiro xaalad <4 ℃ ah.
2× Aad u FududTMMix(UNG)-Heparin, haddii si joogto ah loo isticmaalo, waxa kale oo lagu kaydin karaa 4°C kaydinta muddada gaaban (isticmaal 10 maalmood gudahood).
6× DNA Loading Buffer, waxa lagu kaydin karaa 4°C ama -20°C muddo dheer.
